On the specificity of tuna-directed primers in PCR-SSCP analysis of fish and meat

Weder, Jürgen K. P.; Rehbein, Hartmut; Kaiser, Klaus-Peter

doi:10.1007/s002170100339

Cited by 30 publications

(31 citation statements)

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“…Differences in mobility through the gel network reflect variations in nucleotide sequences between samples, thereby allowing for species detection. PCR-SSCP assays have been developed to identify numerous fish and seafood species, including salmonids [27], scombroids [4], fish from multiple species groups [28], and clams [29].…”

Section: Dna-based Methodsmentioning

confidence: 99%

DNA‐Based Detection of Commercial Fish Species

Rasmussen

Morrissey

2010

Handbook of Seafood Quality, Safety and Health Applications

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Section: Dna-based Methodsmentioning

confidence: 99%

DNA‐Based Detection of Commercial Fish Species

Rasmussen

Morrissey

2010

Handbook of Seafood Quality, Safety and Health Applications

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“…Differentiation between T. collina and T. pagdeni can also be carried out based on nest architecture and external morphology (Sakagami, 1978;Sakagami et al, 1985). SSCP analysis, which is favored for identifying species origins of various taxa, due to its convenience and cost-effectiveness (Orita et al, 1989;Weder et al, 2001;Klinbunga et al, 2007), was then applied to determine whether nucleotide sequences of CUTc1 in T. pagdeni and T. collina were different. Non-overlapping SSCP patterns between T. collina and T. pagdeni were observed ( Figure 5A).…”

Section: Development Of a Species-specific Marker (Cutc1) For Authentmentioning

confidence: 99%

Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand

Theeraapisakkun¹,

Klinbunga²,

Sittipraneed³

2010

Genet. Mol. Res.

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ABSTRACT. A molecular maker for authenticating species origin of the stingless bee (Trigona collina) was developed. Initially, amplified fragment length polymorphism analysis was made of 11 stingless bee species using 64 primer combinations. A 316-bp band found only in T. collina was cloned and sequenced. A primer pair (CUTc1-F/R) was designed and tested for species-specificity in 15 stingless bee species (239 nests). The expected 259-bp fragment was consistently amplified in all T. collina individuals (134/134 nests, 100%). Cross-species amplification was observed in T. pagdeni (43/51 nests; 84.3%), but not in other species. SSCP analysis of CUTc1 unambiguously differentiated T. collina from T. pagdeni. CUTc1 generated three genotypes in Thai T. collina (134 nests). An AA (259/259 bp) genotype was found in all stingless bees from the north (21 nests) and northeast (32 nests), and 23/28 nests from the Central region, whereas a BB (253/253 bp) genotype was observed in most samples from peninsular Thailand (42/53 nests). Heterozygotes exhibiting the AB (253/259 bp) genotype were observed in 5 of 28 nests from Prachuap Khiri Khan located slightly above the Kra ecotone and 11 of 53 nests originated further south of the Kra ecotone. Genotype distribution patterns of CUTc1 clearly indicated intraspecific population differentiation of Thai T. collina.

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“…In another study of the identification of animal species, PCR with tuna-directed primers amplifiying a 148-bp sequence of the cytochrome b gene resulted in strong, specific ssDNA bands for a number of warm-blooded animal species (Weder et al, 2001). Figure 5.3 gives an example of the suitability of RFLP-SSCP analysis to distinguish between the meat of warm-blooded animals, again with the exception of pig and wild boar.…”

Section: Food From Warm-blooded Animalsmentioning

confidence: 99%