On the significance of the heterogeneity in molecular size of human serum γA-globulins

Vaerman, J.-P.; Fudenberg, H. Hugh; Vaerman, C.; Mandy, William J.

doi:10.1016/0019-2791(65)90006-6

Cited by 53 publications

(9 citation statements)

References 31 publications

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“…Normal, as well as paraproteinemic, serums contain IgA with sedimentation coefficients ranging from 7 to 17S (12, 15). The excellent resolving capacity of the tall agarose columns used in our study permitted a partial separation of high-polymer serum IgA from 7S IgA.…”

mentioning

confidence: 77%

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

Everett

Borcherding

1970

Science

335

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mentioning

confidence: 77%

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

Everett

Borcherding

1970

Science

335

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“…The nonreducing gel contained distinct protein species that migrated more slowly than the major form (Fig. 3B, left) and may represent the known polymeric forms of serum IgA (52).…”

Section: Single-step Affinity Purification Of Serum Iga On Immobilizementioning

confidence: 99%

Isolation and Detection of Human IgA Using a Streptococcal IgA-Binding Peptide

Sandin

Linse

Areschoug

et al. 2002

The Journal of Immunology

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Bacterial proteins that bind to the Fc part of IgG have found widespread use in immunology. A similar protein suitable for the isolation and detection of human IgA has not been described. Here, we show that a 50-residue synthetic peptide, designated streptococcal IgA-binding peptide (Sap) and derived from a streptococcal M protein, can be used for single-step affinity purification of human IgA. High affinity binding of IgA required the presence in Sap of a C-terminal cysteine residue, not present in the intact M protein. Passage of human serum through a Sap column caused depletion of >99% of the IgA, and elution of the column allowed quantitative recovery of highly purified IgA, for which the proportions of the IgA1 and IgA2 subclasses were the same as in whole serum. Moreover, immobilized Sap could be used for single-step purification of secretory IgA of both subclasses from human saliva, with a recovery of ∼45%. The Sap peptide could also be used to specifically detect IgA bound to Ag. Together, these data indicate that Sap is a versatile Fc-binding reagent that may open new possibilities for the characterization of human IgA.

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“…First, serum proteins of the y A class have been shown to be made up of heavy and light chains. The light chains of either normal or myeloma yAimmunoglobulins are similar to those of yM-and yGimmunoglobulins but their heavy chains are different from those present in other classes when examined by electrophoresis or by fingerprinting (Carbonara and Heremans, 1963;Cohen and Porter, 1964b;Vaerman et al, 1965). Second, both human and mouse serum yA-immunoglobulins have been shown to be heterogeneous by sedimentation analysis (Vaerman et al, 1965;Fahey, 1961).…”

mentioning

confidence: 99%

Polypeptide Chain Structure of Rabbit Immunoglobulins. III. Secretory γA-Immunoglobulin from Colostrum^*

Cebra¹,

Small²

1967

Biochemistry

139

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On the significance of the heterogeneity in molecular size of human serum γA-globulins

Cited by 53 publications

References 31 publications

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

Isolation and Detection of Human IgA Using a Streptococcal IgA-Binding Peptide

Polypeptide Chain Structure of Rabbit Immunoglobulins. III. Secretory γA-Immunoglobulin from Colostrum^*

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On the significance of the heterogeneity in molecular size of human serum γA-globulins

Cited by 53 publications

References 31 publications

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

L-Dopa: Effect on Concentrations of Dopamine, Norepinephrine, and Serotonin in Brains of Mice

Isolation and Detection of Human IgA Using a Streptococcal IgA-Binding Peptide

Polypeptide Chain Structure of Rabbit Immunoglobulins. III. Secretory γA-Immunoglobulin from Colostrum*

Contact Info

Product

Resources

About

Polypeptide Chain Structure of Rabbit Immunoglobulins. III. Secretory γA-Immunoglobulin from Colostrum^*