On the separation mechanism of capillary sodium dodecyl sulfate‐gel electrophoresis of proteins

Guttman, András

doi:10.1002/elps.1150160198

Cited by 41 publications

(45 citation statements)

References 34 publications

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“…1a). 12 The pore size x strongly depends on total monomer (%T) and crosslinker concentration (%C, weight percentage of crosslinker in total acrylamide content of a gel). Once polymerized, the pore structure of a crosslinked gel is static ("chemical gel", Fig.…”

Section: Microanalytical Separation Mechanisms In Crosslinked Gels Anmentioning

confidence: 99%

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Polymer sieving matrices in microanalytical electrophoresis

Chung

Kim

Herr

2014

Analyst

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Microfluidic design has advanced existing protein separation capabilities and supported novel assays. Key metrics for successful protein separations include fast, robust, and sensitive analysis of complex mixtures of bio-macromolecules. Attaining high separation resolution is a chief concern. Here we review recent advances in polymer-based electrophoresis sieving materials that are impacting microfluidic bioanalytical applications. Looking forward, we comment on unmet needs for advanced separation media in microto-nanoscale devices.

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Section: Microanalytical Separation Mechanisms In Crosslinked Gels Anmentioning

confidence: 99%

“…7,12 Three different separation mechanisms describe electrophoretic migration through a polymer network, depending on shape and size of a protein relative to the pore size and applied electric eld (Fig. 2).…”

mentioning

confidence: 99%

Polymer sieving matrices in microanalytical electrophoresis

Chung

Kim

Herr

2014

Analyst

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“…We used L-PA with the MW range (600 -1000 kDa); other ranges were not tested. According to Guttman's report, 25 the larger MW of PEG used as an entangled polymer for SBPS in CE would make the separation mechanism of SDS-PC fit to the reptation model rather than the Ogston model. Thus, in the case of larger MW of L-PA used in separation of SDS-PC, the separation mechanism may be different from the Ogston model, which needs to be further investigated.…”

Section: Separation Property Of Entangled L-pa Solutionmentioning

confidence: 99%

Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix

et al. 2008

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Poly(methylmethacrylate) (PMMA) microchip electrophoresis of sodium dodecyl sulfate-protein complexes (SDS-PC) using linear-poly(acrylamide) (L-PA) as a separation matrix was investigated. Prior to electrophoresis, channel walls of PMMA were modified with methylcellulose (MC) to prevent adsorption between channel walls and SDS-PC. Size-based protein separation (SBPS) was successfully performed using the MC-coated microchips with Ferguson plot-fittings. The entangled L-PA solution provided high resolution of peaks of SDS-PC when the concentration of L-PA was increased. Some investigations into the separation mechanism, such as the plot of the logarithm of mobility of each SDS-PC versus the logarithm of the molecular weight of the complex exhibiting linear behavior, indicated that the separation mechanism was dependent on mass discrimination, in accordance with Ogston model.

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“…Moreover, only a few studies focused on simultaneously measuring the mobility and dispersion to understand the electrophoretic migration mechanism of proteins. Guttman [38] performed SDS-CGE experiments using polyethylene oxides as the separation matrix. The study aimed to clarify whether the separation can be described by either the Ogston sieving theory, or the reptation or reptation with stretching theory.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic migration of proteins in semidilute polymer solutions

et al. 2008

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We present a systematic study of the electrophoretic migration of 10-200 kDa protein fragments in dilute-polymer solutions using microfluidic chips. The electrophoretic mobility and dispersion of protein samples were measured in a series of monodisperse polydimethylacrylamide (PDMA) polymers of different molecular masses (243, 443, and 764 kDa, polydispersivity index <2) of varying concentration. The polymer solutions were characterized using rheometry. Prior to loading onto the microchip, the polymer solution was mixed with known concentrations of SDS (SDS) surfactant and a staining dye. SDS-denatured protein samples were electrokinetically injected, separated, and detected in the microchip using electric fields ranging from 100 to 300 V/cm. Our results show that the electrophoretic mobility of protein fragments decreases exponentially with the concentration c of the polymer solution. The mobility was found to decrease logarithmically with the molecular weight of the protein fragment. In addition, the mobility was found to be independent of the electric field in the separation channel. The dispersion is relatively independent of polymer concentration and it first increases with protein size and then decreases with a maximum at about 45 kDa. The resolution power of the device decreases with concentration of the PDMA solution but it is always better than 10% of the protein size. The protein migration does not seem to correspond to the Ogston or the reptation models. A semiempirical expression for mobility given by van Winkle fits the data very well.

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On the separation mechanism of capillary sodium dodecyl sulfate‐gel electrophoresis of proteins

Cited by 41 publications

References 34 publications

Polymer sieving matrices in microanalytical electrophoresis

Polymer sieving matrices in microanalytical electrophoresis

Poly(methylmethacrylate) Microchip Electrophoresis of Proteins Using Linear-poly(acrylamide) Solutions as Separation Matrix

Electrophoretic migration of proteins in semidilute polymer solutions

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