On the semi-quantification of polycyclic aromatic hydrocarbons in contaminated soil by an enzyme-linked immunosorbent assay kit

Nording, Malin L.; Frech, Kristina; Persson, Ylva; Forsman, Mats; Haglund, Peter

doi:10.1016/j.aca.2005.08.051

Cited by 15 publications

(6 citation statements)

References 19 publications

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“…As stated earlier, there are threatening occupational health and safety concerns associated with sample preparation. Preparing samples for TPH and/or PAH analysis with the gravimetric method [42,43], IMA ELISA test kits (68,95), laboratory-based IR spectroscopy On the other hand, the portable IR devices such as the Agilent ® 4100 ExoScan FTIR (96), the vis-NIR devices, fluorescence devices such as the UVF 3100 (73) and Raman devices employ electromagnetic radiation for property elucidation. In Raman spectroscopy, the electromagnetic radiation is capable of penetrating glass and plastic containers enabling in-vessel analysis thereby eliminating or reducing possible exposure to hazardous chemicals (97) but, operators still face possible exposure to fugitive lasers necessitating a Class 3B laser warning on the devices to comply with regulations.…”

Section: Occupational Health and Safetymentioning

confidence: 99%

Determination of Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) in Soils: A Review of Spectroscopic and Nonspectroscopic Techniques

Okparanma

Mouazen

2013

Applied Spectroscopy Reviews

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In the analysis of petroleum hydrocarbon-contaminated soils for total petroleum hydrocarbons (TPH) and polycyclic aromatic hydrocarbons (PAH), the role spectroscopic and non-spectroscopic techniques play are inseparable. Therefore, spectroscopic techniques cannot be discussed in isolation. In this report, spectroscopic techniques including Raman, fluorescence, infrared, visible-near-infrared spectroscopies, as well as mass spectroscopy (coupled to a gas chromatograph), and non-spectroscopic techniques such as gravimetric, immunoassay and gas chromatography with flame ionization detection are reviewed. To bridge the perceived gap in coverage of the quantitative applications of the vis-NIR spectroscopy in the rapid determination of TPHs and PAHs in soils, a detailed review of studies from the period 1999-2012 are presented. This report also highlights the strength and limitations of these techniques and evaluates their performance from the perspectives of their attributes of general applicability, namely: economic, portability, operational time, accuracy, and occupational health and safety considerations. Overall, the fluorescence spectroscopic technique had the best performance (85% total score) in comparison to others, while the gravimetric technique performed the least (60% total score). Method-specific solutions geared towards performance improvement have also been suggested.

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Section: Occupational Health and Safetymentioning

confidence: 99%

Determination of Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) in Soils: A Review of Spectroscopic and Nonspectroscopic Techniques

Okparanma

Mouazen

2013

Applied Spectroscopy Reviews

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“…A few test kits have been commercialized to determine total PAHs content in soil and water. [11][12][13][14][15][16][17][18][19] One paper reported on the use of two test kits for detecting oil-contamination on the plumage of seabirds. 20 Only a few papers focus on the generation of antibodies that are specific for individual PAH compounds.…”

Section: Introductionmentioning

confidence: 99%

Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a]pyrene in potable water

Matschulat

Deng

Nießner

et al. 2005

Analyst

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In Europe, a limit value of 10 ng L(-1) was set by the European Commission for benzo[a]pyrene (B[a]P) in water intended for human consumption (Council Directive 98/83/EC) and, therefore, sensitive and reliable methods are needed to evaluate its presence. We report here on the development of a highly sensitive indirect competitive ELISA for the detection of B[a]P in potable water. Fourteen monoclonal antibodies were generated in mice using novel B[a]P derivatives. The immunoassay with the least interference and the best sensitivity was optimized and characterized. As co-solvent, ten percent methanol (v/v) was determined as the optimum concentration for B[a]P solubilization for use with the developed ELISA. With the purified antibody (clone 22F12) the average IC50 for B[a]P and corresponding detection limit at a signal:noise (S/N) ratio of 3 was 65 ng L(-1) and 24 ng L(-1), respectively. From the 16 EPA-designated PAHs, only chrysene, indeno[1,2,3-cd]pyrene, and benzo[b]fluoranthene showed a cross-reactivity (CR) higher than 20%. No CR was observed for two- and three-ringed aromatics as well as dibenz[ah]anthracene and benzo[ghi]perylene. The effect of pH value (range 6.5-9.5), ionic strength (specific electric conductivity 1 microS cm(-1)-2.5 mS cm(-1)), and inorganic ions (sodium, copper, iron, aluminium, manganese, chloride, sulfate, nitrate, and nitrite at maximum permissible levels according to the Council Directive) on both signal and sensitivity of the ELISA was studied. No significant influence of these parameters on the ELISA competition curve was found. We suggest that the optimized ELISA can be used to monitor potable water samples without previous extraction from the samples. The assay should facilitate the cleanup of B[a]P contaminated sites where B[a]P levels fall close to the limit value of the new drinking water directive.

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“…Technological constraint is also one of the factors that has become a hindrance to agro monitoring, although there are many methods, such as chromatography techniques [4][5][6][7][8], surface plasmon resonance (SPR) [9][10][11][12], enzyme-linked immunosorbent assay (ELISA) [13][14][15][16][17], UV-spectroscopy [18], sensor arrays [19], photo acoustic sensors [20], mass spectrometry [21], biosensors [22], bio-optoelectronics [23] and acoustic plate mode sensors [24], which can be used to estimate the components in soils, including water, but all the methods suffer from various drawbacks. The most common drawback within these detection methods is that they often involve laborious measuring steps causing the detection process to take a long time to complete.…”

Section: 10 Introductionmentioning

confidence: 99%

Water Content Estimation in Soils Using Novel Planar Electromagnetic Sensor Arrays

et al. 2013

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Agriculture is one of the important sectors for food supplies. Therefore, a tool for monitoring the agro-environment is important in order to maintain the permanence of agricultural soils. This paper suggests an alternative method for the detection of water content in soils by developing a sensor array with a combination of planar meander and interdigital electromagnetic sensors. The study involved sensor array fabrication using the printed circuit board (PCB) method. The experimental setup consisted of a frequency waveform generator and a signal oscilloscope to collect and analyse the sensors’ output, with VEE Agilent software used to establish the interface. A set of experiments was conducted to determine the relationship between the sensors’ output and the soils’ parameters. The performance of the system was observed where the sensors were tested with the addition of various kinds of soil samples with different concentrations of water content. The sensitivity of the developed sensors was evaluated where the best sensor was selected. Based on the outcomes of the experiments, the Y sensor array placement has the highest sensitivity and can be used to measure the water content in the soils where the data accuracy is compared

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On the semi-quantification of polycyclic aromatic hydrocarbons in contaminated soil by an enzyme-linked immunosorbent assay kit

Cited by 15 publications

References 19 publications

Determination of Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) in Soils: A Review of Spectroscopic and Nonspectroscopic Techniques

Determination of Total Petroleum Hydrocarbon (TPH) and Polycyclic Aromatic Hydrocarbon (PAH) in Soils: A Review of Spectroscopic and Nonspectroscopic Techniques

Development of a highly sensitive monoclonal antibody based ELISA for detection of benzo[a]pyrene in potable water

Water Content Estimation in Soils Using Novel Planar Electromagnetic Sensor Arrays

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