On the self-Affinity of Heparan Sulfates from Quiescent or Proliferating Normal 3T3 Cells and from SV40-Transformed Cells.

Fransson, Lars‐Âke; Rosso, Mario Del; Thell, Lars; Yang, Qian; Datema, Roelf; Chattopadhyaya, J.

doi:10.3891/acta.chem.scand.39b-0163

Cited by 6 publications

(4 citation statements)

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“…It should be noted that heparan sulfate from growing hepatoma cells can stimulate growth of serumstarved cells, but is without effect on cells in active growth phase (Fedarko et al, 1989). The structure and interactive properties of endogeneous cell surface heparan sulfates are also altered when cells enter the proliferative state or when they become transformed (Fransson et al, 1981(Fransson et al, , 1982Fransson and Del ROSSO, 1985; see also Gallagher and Lyon, 1989). In general, heparan sulfates from postconfluent, nongrowing cells appear to inhibit growth (Fritze et al, 1985;Fedarko et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Proliferation of cultured fibroblasts is inhibited byL‐Iduronate—containing glycosaminoglycans

Westergren‐Thorsson

Onnervik

Fransson

et al. 1991

Journal Cellular Physiology

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We have modified a method (Gilles et al: Anal. Biochem., 159:109-113, 1986) for measuring cell number, that is based on the binding of crystal violet to cell nuclei and used it to assay effects of various glycosaminoglycans on growth of human lung fibroblasts. The procedure was modified by growing cells in microcultures (96 well microplates) and by measuring the amount of adsorbed dye using a microplate photometer after solubilisation of the cells with detergent. There was a linear relationship between absorbance and cell number measured by a Coulter Counter. The method is rapid and sensitive and can be used for screening many samples as well as measuring growth rates at low initial cell densities. Even the low growth rates obtained in the absence of serum can be detected. Human lung fibroblasts were growth arrested by serum deprivation and then grown for periods of up to 4 days in the presence of serum and exogenously added glycosaminoglycans (range, 0.1-100 micrograms/ml). Hyaluronan, chondroitin sulfate, and dextran sulfate were without effects, whereas dermatan sulfate, certain heparan sulfates, and heparin suppressed growth (20%-50% inhibition). The antiproliferative activity of dermatan sulfate increased with increasing iduronate content. Certain heparan sulfates, with moderately high sulfate and L-iduronate contents were better inhibitors than heparin despite the fact that the latter glycan has even higher sulfate and L-iduronate contents. The antiproliferative effect of exogenous glycans appeared after a lag period of 3-4 days, suggesting that they interfered with factors produced by the cells. Furthermore, the degree of inhibition was greater when the initial cell density was low. Above a certain level of seeded cells (approx. 10,000 cells/well), there was no inhibition by any of the glycosaminoglycans. It is possible that exogenous glycans cannot overcome endogenous growth-promoting effects in densely seeded cultures.

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Section: Discussionmentioning

confidence: 99%

Proliferation of cultured fibroblasts is inhibited byL‐Iduronate—containing glycosaminoglycans

Westergren‐Thorsson

Onnervik

Fransson

et al. 1991

Journal Cellular Physiology

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“…Given differences in HS composition and structure from one cell type to another [49], this self-association may play a major role in cell-cell recognitions [49] and interactions of cell surface PGs with those of HS-PGs in basement membranes. In quiescent cells HS-PG is retained on the cell surface, but during growth it is lost and that which remains has a reduced self-affinity [60]. This would fit with a loss of cell-cell interaction which is required for cells to divide and multiply.…”

Section: Heparan Sulphate Proteoglycansmentioning

confidence: 99%

“…This would fit with a loss of cell-cell interaction which is required for cells to divide and multiply. In tumour cells, which are usually rapidly growing, this PG is also deficient at the cell surface [60] and the HS shows either no self-association [64] or lacks affinity for one particular subtype, as is observed in normal cells [60]. Together these observations point to the importance of these PGs in cell-cell and cell-environmental interactions, and that their organization is changed and fundamentally disturbed in tumour cells resulting in changes in cellular behaviour.…”

Section: Heparan Sulphate Proteoglycansmentioning

confidence: 99%

Proteoglycans in health and disease: structures and functions

Poole¹

1986

Biochemical Journal

435

197

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“…Growing cells, which should be less adhesive, contain cell-surface HSPG with heparan sulfate chains that show little tendency to self-associate. 123 Proteoglycans also appear to induce the formation of gap junctions in primary liver cultures by stimulating the synthesis of a specific gap junction protein. 124 It remains to be seen whether a similar situation exists for other types of cells such as vascular endothelial and smooth muscle cells.…”

Section: Cell Adhesionmentioning

confidence: 99%