On the role of the spore cell wall in the activation ofPhycomyces blakesleeanus spores

Verbeke, Marc N.; Laere, André J. Van

doi:10.1016/0147-5975(82)90073-1

Cited by 7 publications

(3 citation statements)

References 12 publications

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“…2) and to decrease in density (Van Laere et al, 1 9 8 0~) during the first 30 min of germination. A loosening of the cell wall might also be involved in this process (Verbeke & Van Laere, 1982, 1986, but it remains difficult to distinguish between cause and effect in this respect.…”

Section: Role Of Trehalose Breakdownmentioning

confidence: 99%

Relation between Germination, Trehalose and the Status of Water in Phycomyces blakesleeanus Spores as Measured by Proton-NMR

et al. 1987

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The distribution and properties of the cellular water in sporangiospores of Phycomyces blakesleeanus were investigated using proton-NMR. In dormant spores different classes of water were characterized by a difference in their transverse relaxation times (T2). The amount of cytoplasmic water was estimated to be as low as about 700 mg (g dry wt)-' and its small T2 (1 8.2 ms) indicated a very limited mobility. About 10 min after induction of germination (by a heat shock or by addition of 0.1 M-acetate), both the content and the mobility of the cytoplasmic water increased sharply. These changes coincided with a rapid breakdown of most of the cellular trehalose and with the production (and leakage from the spores) of large amounts of glycerol. The role of these biochemical changes is discussed in relation to the water status of the spores.

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Section: Role Of Trehalose Breakdownmentioning

confidence: 99%

Relation between Germination, Trehalose and the Status of Water in Phycomyces blakesleeanus Spores as Measured by Proton-NMR

et al. 1987

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“…The increases are inhibited by cycloheximide and 5-fluorouracil, suggesting that protein neosynthesis on mRNA formed during germination is involved. A loosening of the spore cell wall is indicated indirectly by the swelling of the spores, by their increased resistance to artificial hypotonic shocks [55] and their water relations at different environmental water potentials [56]. A loosening of the spore cell wall is indicated indirectly by the swelling of the spores, by their increased resistance to artificial hypotonic shocks [55] and their water relations at different environmental water potentials [56].…”

Section: Cell Wallmentioning

confidence: 99%

“…Ultrastructural changes in the cell wall include an extension of the old wall accompanied by a thickening of the innermost layer, which finally protrudes through the old wall to form the germ tube [7]. A loosening of the spore cell wall is indicated indirectly by the swelling of the spores, by their increased resistance to artificial hypotonic shocks [55] and their water relations at different environmental water potentials [56].…”

Section: Cell Wallmentioning

confidence: 99%

Biochemistry of spore germination in Phycomyces

Laere¹

1986

FEMS Microbiology Letters

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The constitutionally dormant spores of Phycomyces blakesleeanus can be activated by heat shock or treatment with several monocarboxylic acids. Activation is followed first by a general stimulation of metabolism, e.g. respiration, protein‐, RNA‐ and cell‐wall synthesis, and subsequently by nuclear division and germ‐tube emergence. Initial germination is not dependent on RNA synthesis and can even start without protein synthesis. The first common effect of different activating treatments is a transient rise in cyclic AMP (cAMP) content, caused by a change in phosphodiesterase activity after heat activation, and by unknown factors during activation by acids. cAMP transiently activates trehalase and glycerol‐3‐phosphatase in the spores. The activation of these enzymes causes a quick turnover of trehalose into glycerol. During the same period, the water status of the cells is altered so dramatically that perhaps this may explain at least part of the stimulation of metabolism in the germinating spore.

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Osmotic Adjustment and the Role of Turgor in Mycelial Fungi

Money

1994

Growth, Differentiation and Sexuality

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On the role of the spore cell wall in the activation ofPhycomyces blakesleeanus spores

Cited by 7 publications

References 12 publications

Relation between Germination, Trehalose and the Status of Water in Phycomyces blakesleeanus Spores as Measured by Proton-NMR

Relation between Germination, Trehalose and the Status of Water in Phycomyces blakesleeanus Spores as Measured by Proton-NMR

Biochemistry of spore germination in Phycomyces

Osmotic Adjustment and the Role of Turgor in Mycelial Fungi

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