On the role of Nb-related sites of an oxidized β-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells

Jirka, Ivan; Vandrovcová, Marta; Frank, Otakar; Tolde, Zdeněk; Plšek, Jan; Luxbacher, Thomas; Bačáková, Lucie; Starý, V.

doi:10.1016/j.msec.2012.12.073

Cited by 69 publications

(58 citation statements)

References 42 publications

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“…Increased adhesion and spreading of human mesenchymal stromal cells on the Piranha etched surface of a Ti40Nb alloy containing an Nb 2 O 5 phase relative to the adhesion and spreading of the cells on the etched surface of Ti with the same surface topography has recently been observed [22]. In line with our previous results [4], the increased biocompatibility of the alloy surface was attributed to the presence of the surface Nb 2 O 5 phase, which strongly affects the surface energy and the charge of the alloy. To test the role of the surface charge in the samples in our study, a discussion of other effects, including surface morphology, topography and crystal structure should be included.…”

Section: Discussionsupporting

confidence: 91%

“…While the MG-63 cell numbers on the Ti and Nb samples on day 1 after seeding were similar to the values on the polystyrene dishes, the numbers on TiNb were 1.4 (LT series) to 2.8 times higher (HT series, [4]) than on polystyrene. A further increase in the number of MG-63 cells proceeded on day 3 after seeding.…”

Section: Discussionsupporting

confidence: 49%

“…the alloy sample contains a larger number of positively charged (basic) sites, possibly due to the presence of defective tetrahedrally coordinated Nb 5+ species acting as positively-charged Lewis centers [4]. The presence of these surface sites in the alloy sample was used to explain the higher number of osteoblast-like MG-63 cells observed on TiNb600 than on Ti600 .…”

Section: Discussionmentioning

confidence: 99%

“…improved cell adhesion and proliferation, particularly on their oxidized surfaces [4]. The interaction of the cells with the surface of a solid sample is mediated by extracellular matrix (ECM) molecules, which are mainly proteins spontaneously adsorbed to the material surface from biological fluids, such as blood, interstitial fluid or cell culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

et al. 2014

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An investigation was made of the adhesion, growth and differentiation of osteoblast-like MG-63 and Saos-2 cells on titanium (Ti) and niobium (Nb) supports and on TiNb alloy with surfaces oxidized at 165°C under hydrothermal conditions and at 600°C in a stream of air. The oxidation mode and the chemical composition of the samples tuned the morphology, topography and distribution of the charge on their surfaces, which enabled us to evaluate the importance of these material characteristics in the interaction of the cells with the sample surface. Numbers of adhered MG-63 and Saos-2 cells correlated with the number of positively-charged (related with the Nb2O5 phase) and negatively-charged sites (related with the TiO2 phase) on the alloy surface. Proliferation of these cells is correlated with the presence of positively-charged (i.e. basic) sites of the Nb2O5 alloy phase, while cell differentiation is correlated with negatively-charged (acidic) sites of the TiO2 alloy phase. The number of charged sites and adhered cells was substantially higher on the alloy sample oxidized at 600°C than on the hydrothermally treated sample at 165°C. The expression values of osteoblast differentiation markers (collagen type I and osteocalcin) were higher for cells grown on the Ti samples than for those grown on the TiNb samples. This was more particularly apparent in the samples treated at 165°C. No considerable immune activation of murine macrophage-like RAW 264.7 cells on the tested samples was found. The secretion of TNF-α by these cells into the cell culture media was much lower than for either cells grown in the presence of bacterial lipopolysaccharide, or untreated control samples. Thus, oxidized Ti and TiNb are both promising materials for bone implantation; TiNb for applications where bone cell proliferation is desirable, and Ti for induction of osteogenic cell differentiation.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

et al. 2014

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“…The new perspective group of materials is the titanium β alloys -Ti alloys with β structure, usually containing Nb, Zr, Ta and other elements. These show lower modulus of elasticity, higher bio-compatbility but are more expensive on the other hand [2,4,5].…”

Section: Introductionmentioning

confidence: 99%

The Influence of Titanium-Niobium Alloys on the Growth of the Oxide Layer

Krčil

Mára

Sobotová

et al. 2017

APP

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Abstract. This paper discusses issues of growth of a thin oxide layer formed on the surface of titanium alloys. The oxide layer on the surface introduces a corrosion resistance and a bio-compatibility, both of which are required for medical applications. The oxide layer is a result of a spontaneous passivation, but for the practical applications it is necessary to control the growth of oxides. In present work, the oxide layer was formed on the CP Ti grade 2, Ti39Nb alloy and CP Ti grade 2 with Ti39Nb PVD coating of two different thicknesses by a thermal oxidation at 600• C for 8 hours. After the oxidation process the oxide layer was observed by SEM.

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Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

Dai

Xiong

et al. 2023

Advanced Science

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The rapid degradation of magnesium (Mg) alloy implants erodes mechanical performance and interfacial bioactivity, thereby limiting their clinical utility. Surface modification is among the solutions to improve corrosion resistance and bioefficacy of Mg alloys. Novel composite coatings that incorporate nanostructures create new opportunities for their expanded use. Particle size dominance and impermeability may increase corrosion resistance and thereby prolong implant service time. Nanoparticles with specific biological effects may be released into the peri‐implant microenvironment during the degradation of coatings to promote healing. Composite nanocoatings provide nanoscale surfaces to promote cell adhesion and proliferation. Nanoparticles may activate cellular signaling pathways, while those with porous or core–shell structures may carry antibacterial or immunomodulatory drugs. Composite nanocoatings may promote vascular reendothelialization and osteogenesis, attenuate inflammation, and inhibit bacterial growth, thus increasing their applicability in complex clinical microenvironments such as those of atherosclerosis and open fractures. This review combines the physicochemical properties and biological efficiency of Mg‐based alloy biomedical implants to summarize the advantages of composite nanocoatings, analyzes their mechanisms of action, and proposes design and construction strategies, with the purpose of providing a reference for promoting the clinical application of Mg alloy implants and to further the design of nanocoatings.

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On the role of Nb-related sites of an oxidized β-TiNb alloy surface in its interaction with osteoblast-like MG-63 cells

Cited by 69 publications

References 42 publications

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

Interaction of Human Osteoblast-Like Saos-2 and MG-63 Cells with Thermally Oxidized Surfaces of a Titanium-Niobium Alloy

The Influence of Titanium-Niobium Alloys on the Growth of the Oxide Layer

Composite Nanocoatings of Biomedical Magnesium Alloy Implants: Advantages, Mechanisms, and Design Strategies

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