On the role of methacrylic acid copolymers in the intracellular delivery of antisense oligonucleotides

The endocytic pathway and therapeutic efficiency of doxorubicin conjugated cholesterol-derived polymers

“…The Alexa Flour dye content of 8 mol% CMA-modified copolymer was found to be 0.22 mol%; which is sufficient for effective detection by confocal microscopy analysis. 26 The internalization profile of P(MAA-co-CMA) Confocal laser scanning microscopy was employed to visualize the cell uptake profile of the P(MAA-co-CMA) copolymer series in both human neuroblastoma (SHEP) and human liver hepatocellular carcinoma (HepG2) cell lines. HepG2 cells were selected due to their well-known cholesterol recognition profile.…”

Development of pH–responsive nanocarriers using trimethylchitosans and methacrylic acid copolymer for siRNA delivery

“…It has been shown for MAA that polyelectrolytes bearing carboxylic acid group units undergo coil-to-globule conformational transition upon a decrease in pH (Fig. 3) [58]. The state of ionization determines the ability of the polymer to interact/ destabilize lipid membrane.…”

pH-sensitive immunoliposomes specific to the CD33 cell surface antigen of leukemic cells

“…Most polymers investigated so far for the design of pH-sensitive liposomes are based on poly(alkyl acrylic acid)s (Chen et al, 1999;Jones et al, 2003;Kyriakides et al, 2002), succinylated PEG (Kono et al, 1994;Mizoue et al, 2002), biodegradable polyphosphazenes (CouffinHoarau and and N-isopropylacrylamide (NIPAM) copolymers (Meyer et al, 1998;Roux et al, 2002aRoux et al, , 2003Zignani et al, 2000). For example, poly(alkyl acrylate-co-methacrylic acid)s have been shown to express strong membrane destabilizing properties (Yessine et al, 2003) and have been used to construct pH-responsive lipoplexes (Yessine et al, 2006) and polyion complex micelles (Yessine et al, 2007) that were able to facilitate the transfer of oligonucleotides from the endosomes to the cytoplasm.…”