On the role of a Lipid-Transfer Protein. Arabidopsis <i>ltp3</i> mutant is compromised in germination and seedling growth.

Pagnussat, Luciana A.; Oyarburo, Natalia Soledad; Cimmino, Carlos; Pinedo, Marcela; Canal, Laura de la

doi:10.1080/15592324.2015.1105417

Cited by 15 publications

(11 citation statements)

References 44 publications

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“…Consistent with this hypothesis, GhADH1 was significantly up-regulated in GhWRKY6 -VIGS lines, which ultimately resulted in enhanced salt tolerance. The transcript level of lipid transfer protein 3 ( LTP3 , Gh_A10G1332), which responds to salt, drought, cold, and ABA, increased from 26 to 1,000 FPKM (Guo et al, 2013; Pagnussat et al, 2015; Gao et al, 2016). However, the promoter region of LTP3 contained no W-box, indicating that GhWRKY6 cannot directly bind to its promoter.…”

Section: Discussionmentioning

confidence: 99%

GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

Zhou

et al. 2019

Front. Genet.

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Drought and high salinity are key limiting factors for cotton production. Therefore, research is increasingly focused on the underlying stress response mechanisms of cotton. We first identified and cloned a novel gene encoding the 525 amino acids in cotton, namely GhWRKY6 . qRT-PCR analysis indicated that GhWRKY6 was induced by NaCl, PEG 6000 and ABA. Analyses of germination rate and root length indicated that overexpression of GhWRKY6 in Arabidopsis resulted in hypersensitivity to ABA, NaCl, and PEG 6000. In contrast, the loss-of-function mutant wrky6 was insensitive and had slightly longer roots than the wild-type did under these treatment conditions. Furthermore, GhWRKY6 overexpression in Arabidopsis modulated salt- and drought-sensitive phenotypes and stomatal aperture by regulating ABA signaling pathways, and reduced plant tolerance to abiotic stress through reactive oxygen species (ROS) enrichment, reduced proline content, and increased electrolytes and malondialdehyde (MDA). The expression levels of a series of ABA-, salt- and drought-related marker genes were altered in overexpression seedlings. Virus-induced gene silencing (VIGS) technology revealed that down-regulation of GhWRKY6 increased salt tolerance in cotton. These results demonstrate that GhWRKY6 is a negative regulator of plant responses to abiotic stress via the ABA signaling pathway.

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Section: Discussionmentioning

confidence: 99%

GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

Zhou

et al. 2019

Front. Genet.

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“…Log 2 Ratios Functions References WRI1 (AT3G54320) 0.69 Promoting oil accumulation Focks and Benning, 1998;Cernac and Benning, 2004;Masaki et al, 2005;Baud et al, 2007;Maeo et al, 2009;To et al, 2012 BCCP1 (AT2G38040) 0.63 Promoting oil accumulation Ohlrogge et al, 1995;Sasaki et al, 1995;Mu et al, 2008;Li et al, 2011 CAC3 (AT2G38040) 0.66 Promoting oil accumulation Ohlrogge et al, 1995;Sasaki et al, 1995;Mu et al, 2008;Li et al, 2011 MCAMT (AT2G30200) 0.60 Promoting oil accumulation Jung et al, 2019;Mu et al, 2008 PLIP1 (AT3G61680) 0.65 Promoting oil accumulation Wang et al, 2017a GPAT2 (AT1G02390) 0.96 Exhibiting sn-1 and sn-2 acyltransferase activities and utilizing dicarboxylic acyl-CoA as the substrate for the biosynthesis of the extracellular lipids Beisson et al, 2007;Yang et al, 2012;Jayawardhane et al, 2018 LTP3 (AT5G59320) 2.42 Promoting soluble sugar accumulation and enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains with no obvious effect on oil accumulation in the single mutant Kader, 1996;Arondel et al, 2000;Pagnussat et al, 2015;Wong et al, 2017 LTP4 (AT5G59310) 1.19 Enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains Kader, 1996;Arondel et al, 2000;Wong et al, 2017 LTP5 (AT3G51600) 1.06 Enhancing the in vitro transfer of phospholipids between membranes and binding acyl chains anthocyanins, and proanthocyanidins (PAs; Lepiniec et al, 2006). Considering that biosynthesized flavonols are converted into both anthocyanins and PAs in the flavonoid biosynthetic pathway (Lepiniec et al, 2006), we speculated that anthocyanins and PAs compete against each other for flavonols during flavonoid biosynthesis.…”

Section: Degsmentioning

confidence: 99%

“…PLIP1, as a chloroplast thylakoid-associated protein, functions in the export of FAs from the chloroplast and the incorporation of FAs derived from the thylakoid membrane lipid pool into TAG, and positively regulates FA accumulation in Arabidopsis seeds (Wang et al, 2017a). The LTP3 lossof-function mutant seeds contain oil content similar to wild-type plants (Pagnussat et al, 2015). However, LTP3, LTP4, and LTP5 can enhance the in vitro transfer of phospholipids between membranes and can bind acyl chains (Kader, 1996;Arondel et al, 2000;Wong et al, 2017); thus, they probably have a redundant function in seed oil accumulation.…”

Section: Flavonoids Including Anthocyanins and Pas (Figs 4 And 8; Smentioning

confidence: 99%

Melatonin Represses Oil and Anthocyanin Accumulation in Seeds

Liu

Guo

et al. 2020

Plant Physiol.

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“…During germination, seed proteins are broken down by proteases to produce nitrogen for the biosynthesis of other substances (Martinez et al 2019). It has been shown that protease inhibitor activities such as protease inhibitor/seed storage/LTP family decreases during the germination process (Pagnussat et al 2015). Other associated gene was a nodulin with possible function in germination in Arabidopsis (Denancé et al 2014).…”

Section: Associated Genes With Rdwmentioning

confidence: 99%

Genome-wide association study (GWAS) of seed germination-related traits in rice and identification of candidate genes

Panhabadi¹,

Ahmadikhah²,

Farokhi³

et al. 2021

Preprint

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Background Genome-wide association study (GWAS) has become an accepted and powerful method for understanding the associations between phenotypes and genotypes. In agricultural production, uniform and rapid germination is an important prerequisite in crop production. Here, a rice (Oryza sativa L.) GWAS with 33,934 SNPs (MAF > 0.05) for eight germination traits including germination percentage (GP), shoot (SL) and root length (RL), root (RFW) and shoot fresh weight (SFW), root (RDW) and shoot (SDW) dry weight, and number of days to germinate (NDG) was performed to define genomic regions influencing seed germination. Results Loci (43) with 70 significant germination-associated markers were detected across all rice chromosomes. Some of novel candidate associated genes were: LOC_Os01g26210 (OsWAK6) co-located with qGR-1 that is seed vigor QTL, LOC_Os07g23944 (GH31) with an α-glucosidases /starch lyase activity; id7000519 marker that corresponds to a gene cluster containing glutathione S-transferase and glucan endo-1,3-β-glucosidase co-located with qAG3 germination-related marker, LOC_Os06g47640 (calmodulin-related calcium sensor protein 29) involved in the inhibition of ABA during seed germination, and id4006430 marker that corresponds to a gene cluster containing three GH17 hydrolytic enzyme that are co-located with qHD4 and qGI1 markers. Conclusion The germination process is an initial and important step in the production of agricultural products, especially for rice, which is a crop plant that is grown in flooded lands. Here, the genetic diversity of rice genotypes was put under scrutiny for germination. Our GWAS results identified several likely candidate genes for germination traits that will greatly contribute to our understanding of the genetic complexity underlying the corresponding traits. The associated genes with the germination traits can be generally classified as hydrolytic enzymes and regulatory proteins that can directly or indirectly influence germination.

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On the role of a Lipid-Transfer Protein. Arabidopsis ltp3 mutant is compromised in germination and seedling growth.

Cited by 15 publications

References 44 publications

GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

GhWRKY6 Acts as a Negative Regulator in Both Transgenic Arabidopsis and Cotton During Drought and Salt Stress

Melatonin Represses Oil and Anthocyanin Accumulation in Seeds

Genome-wide association study (GWAS) of seed germination-related traits in rice and identification of candidate genes

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