On the Ribonucleic Acid Synthesized in a Cell-Free System of Escherichia Coli

Otaka, Eiko; Mitsui, Hiromi; Osawa, Syozo

doi:10.1073/pnas.48.3.425

Cited by 45 publications

(3 citation statements)

References 12 publications

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“…The results obtained (Sagik et al, 1962) were now in agreement, the same wide diversity from above 3 0 s to 8s being observed. Similar findings were soon reported with other systems (Otaka et al, 1962;Monier et al, 1962;Hiatt, 1962).…”

Section: The Size Distribution Of Rna Messages and Its Significancsupporting

confidence: 91%

“…To distinguish one type of RNA molecule from another, recourse was had to the methylated-albumin-kieselguhr (MAK) columns introduced by Lerman (1955) and subsequently developed and used by Mandell and Hershey (1980) to separate DNA from RNA and size fractionation of DNA. Others (Philipson, 1981 ;Otaka et al, 1962) have recently employed these columns to detect and isolate RNA molecules differing in size.…”

Section: F Random Versus Non-random Transcription Of a Dna Genomementioning

confidence: 99%

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The Present Status of the Transfer of Genetic Information and Its Control

Spiegelman¹

1963

Cold Spring Harbor Symposia on Quantitative Biology

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Section: The Size Distribution Of Rna Messages and Its Significancsupporting

confidence: 91%

Section: F Random Versus Non-random Transcription Of a Dna Genomementioning

confidence: 99%

The Present Status of the Transfer of Genetic Information and Its Control

Spiegelman¹

1963

Cold Spring Harbor Symposia on Quantitative Biology

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“…Others'8' 19 have recently employed these columns to detect and isolate RNA molecules differing in size. The discovery by Sueoka and Cheng20 that these columns can recognize base composition of DNA encouraged us to investigate whether a similar recognition occurs with RNA.…”

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confidence: 99%

Evidence for a Nonrandom Reading of the Genome

Kano-Sueoka

Spiegelman

1962

Proc. Natl. Acad. Sci. U.S.A.

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Recently accumulated data suggest that the transcription of an individual cistron into a ribopolynucleotide and its subsequent translation into a polypeptide are ordered and oriented processes. Thus, the ingenious genetic experiments of Crick et al.1 and Champe and Benzer2 are readily interpretable if the reading of the base sequence starts from a fixed point and continues until the end of the cistron is indicated. Further, the elegant analysis of haemoglobin synthesis by Dintzis3 implies that the growth of a polypeptide chain is the result of a sequential addition of residues starting at the NH2-terminal amino acid and finishing at the free carboxyl end.Since every cistron is presumed to have its own beginning, each can, in principle, be read independently of the others. The experiments cited do not, therefore, provide an answer to the following question: Is the transcription of the genome random or nonrandom? By "random" we mean that the probability of transcription for any particular cistron is invariant with time and independent of its location in the genome.The fact that specific proteins appear sequentially in phage-infected bacteria4-7 does not answer the question since one can have an ordered use of randomly produced genetic messages.An approach toward the solution of the problem may be seen by recognizing that if the reading is random, then any sample of RNA messages is equivalent to any other. If it is not random, a sample taken at one time should be distinguishable from that taken at another. To decide between these alternatives, three experimental conditions are required. One is a situation synchronized with respect to the onset in the transcription of a known genome. A second is the suppression of possible selective degradation of particular messages due to use in protein synthesis.

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�bertragung der genetischen Information

Wittmann¹

1963

Naturwissenschaften

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On the Ribonucleic Acid Synthesized in a Cell-Free System of Escherichia Coli

Cited by 45 publications

References 12 publications

The Present Status of the Transfer of Genetic Information and Its Control

The Present Status of the Transfer of Genetic Information and Its Control

Evidence for a Nonrandom Reading of the Genome

�bertragung der genetischen Information

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