On the response of Escherichia coli to high rates of deformation

Fitzmaurice, Brianna; Painter, Jonathan; Appleby-Thomas, Gareth; Wood, David; Hazael, Rachael; McMillan, Paul F.

doi:10.1063/1.4971722

Cited by 4 publications

(2 citation statements)

References 11 publications

(18 reference statements)

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“…The research presented in this paper, following on from initial 1D dynamic loading experiments (Fitzmaurice et al, 2017), focuses on Escherichia coli NCTC 10538, a bacterium typically less well adapted to extreme environments, unlike other more robust organisms such as S. oneidensis and B. subtilis. However, certain strains of E. coli have been found to reproduce even at temperatures up to 49 °C (Fotadar et al, 2005), likely due to mutations of proteins occurring during heating allowing greater survival.…”

Section: Introductionmentioning

confidence: 99%

The effects of quasi-one-dimensional shock on Escherichia coli while controlling pressure and temperature

Fitzmaurice

Appleby-Thomas

Painter

et al. 2021

Icarus

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Section: Introductionmentioning

confidence: 99%

The effects of quasi-one-dimensional shock on Escherichia coli while controlling pressure and temperature

Fitzmaurice

Appleby-Thomas

Painter

et al. 2021

Icarus

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“…When using E. coli (NCTC 9001), Enterococcus faecalis (ATCC 19433) and Zygosaccharomyces bailii (DSM 70492), it was found that with cavitation fully supressed these microorganisms were not affected by shock compression, suggesting that water cavitation might have the greater potential to disrupt the cells. Subsequently, similar experimental techniques [15] were used to study the shock response of different biological systems [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

A new apparatus to induce lysis of planktonic microbial cells by shock compression, cavitation and spray

et al. 2017

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Experiments were conducted on an aqueous growth medium containing cultures of Escherichia coli (E. coli) XL1-Blue, to investigate, in a single experiment, the effect of two types of dynamic mechanical loading on cellular integrity. A bespoke shock tube was used to subject separate portions of a planktonic bacterial culture to two different loading sequences: (i) shock compression followed by cavitation, and (ii) shock compression followed by spray. The apparatus allows the generation of an adjustable loading shock wave of magnitude up to 300 MPa in a sterile laboratory environment. Cultures of E. coli were tested with this apparatus and the spread-plate technique was used to measure the survivability after mechanical loading. The loading sequence (ii) gave higher mortality than (i), suggesting that the bacteria are more vulnerable to shear deformation and cavitation than to hydrostatic compression. We present the results of preliminary experiments and suggestions for further experimental work; we discuss the potential applications of this technique to sterilize large volumes of fluid samples.

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Remediation of pharmacophoric laboratory waste by using biodegradable carbon nanoparticles of bacterial biofilm origin

Savadiya,

Pandey,

Misra

2024

Environmental Research

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On the response of Escherichia coli to high rates of deformation

Cited by 4 publications

References 11 publications

The effects of quasi-one-dimensional shock on Escherichia coli while controlling pressure and temperature

The effects of quasi-one-dimensional shock on Escherichia coli while controlling pressure and temperature

A new apparatus to induce lysis of planktonic microbial cells by shock compression, cavitation and spray

Remediation of pharmacophoric laboratory waste by using biodegradable carbon nanoparticles of bacterial biofilm origin

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