On the reliability of fungal materials used in studies on Ophiocordyceps sinensis

Dong, Chao; Yao, Yi‐Jian

doi:10.1007/s10295-010-0877-4

Cited by 35 publications

(20 citation statements)

References 32 publications

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“…HSM CGB999335 produced optimal growth at 16°C (Fig 4), consistent with previous observations that O . sinensis mycelium grows best at 15 and 18°C [15]. HSM biomass gradually decreased at temperatures above 16°C and limited growth was noticed at higher temperatures, consistent with previous work showing that O .…”

Section: Resultssupporting

confidence: 90%

“…HSM biomass gradually decreased at temperatures above 16°C and limited growth was noticed at higher temperatures, consistent with previous work showing that O . sinensis does not grow at temperatures above 25°C [15]. …”

Section: Resultsmentioning

confidence: 99%

“…These results are in agreement with previous observations that O . sinensis -derived mycelium grows best at pH 6 [15]. …”

Section: Resultsmentioning

confidence: 99%

“…Confusion about the material described as O . sinensis is also rampant in the scientific literature, with a recent study estimating that more than three quarter of studies published on this fungus used unreliable, uncertain, or unspecified material [15]. Several fungal species have been proposed to represent the anamorph mycelium of O .…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Culture and Characterization of Hirsutella sinensis Mycelium from Caterpillar Fungus Fruiting Body

Ko¹,

Liau²,

Lee³

et al. 2017

PLoS ONE

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The caterpillar fungus Ophiocordyceps sinensis (previously called Cordyceps sinensis) has been used for centuries in Asia as a tonic to improve health and longevity. Recent studies show that O. sinensis produces a wide range of biological effects on cells, laboratory animals and humans, including anti-fatigue, anti-infection, anti-inflammatory, antioxidant, and anti-tumor activities. In view of the rarity of O. sinensis fruiting bodies in nature, cultivation of its anamorph mycelium represents a useful alternative for large-scale production. However, O. sinensis fruiting bodies harvested in nature harbor several fungal contaminants, a phenomenon that led to the isolation and characterization of a large number of incorrect mycelium strains. We report here the isolation of a mycelium from a fruiting body of O. sinensis and we identify the isolate as O. sinensis’ anamorph (also called Hirsutella sinensis) based on multi-locus sequence typing of several fungal genes (ITS, nrSSU, nrLSU, RPB1, RPB2, MCM7, β-tubulin, TEF-1α, and ATP6). The main characteristics of the isolated mycelium, including its optimal growth at low temperature (16°C) and its biochemical composition, are similar to that of O. sinensis fruiting bodies, indicating that the mycelium strain characterized here may be used as a substitute for the rare and expensive O. sinensis fruiting bodies found in nature.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

“…These results are in agreement with previous observations that O . sinensis -derived mycelium grows best at pH 6 [15]. …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Isolation, Culture and Characterization of Hirsutella sinensis Mycelium from Caterpillar Fungus Fruiting Body

Ko¹,

Liau²,

Lee³

et al. 2017

PLoS ONE

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“…Several biological activities are based on extract, fractions and polysaccharides and there are discrepancies in structure determination of polysaccharides (Chen et al 2013). The fruit-body production in cultivation has not been successful yet and mycelia culture is the major source of studies (Dong & Yao 2011). In comparison to cultured mycelium, the natural O. sinensis undergoes cascade of events from the selection of host up to the formation of fruiting enduring extreme environmental conditions together with interaction with Cordyceps-associated species.…”

Section: Introductionmentioning

confidence: 99%

Review on Pharmacologically Active Metabolites from Yarsagumba (Ophiocordyceps sinensis), an Epitome of Himalayan Elixir

Shrestha¹,

Shrestha²,

Lee

et al. 2014

Nepal Journal of Science and Technology

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Yarsagumba (Ophiocordyceps sinensis (Berk.) Sung et al.) is a well-known entomogenous fungus native to alpine nival terrain of trans-Himalaya and Tibetan Plateau. The traditional use of O. sinensis in Ayurvedic medicine as well as in traditional Chinese medicine for range of human health requirements with well documented evidences are themselves testimony of its value. It is regarded as winter worm, summer grass due to its association with Thitarodes (Hepialus) larvae. The extremities of habitat condition and endurance of fungus to sustain adversaries by production of metabolites has led to unique profile of metabolites including nucleosides, proteins and nitrogenous compounds, polysaccharides, sterols, fatty acids and their derivatives, as well as some vitamins and inorganics. There are wide ranges of biological activities that have been reported from O. sinensis including anti-inflammatory, antioxidant, anti-tumor, anti-metastatic, immunomodulatory, antimicrobial, insecticidal, hypolipidaemic, hypoglycemic, antiageing, lipolytic, neuroprotective, renoprotective effects, etc. Although several components can be responsible for activity of compounds, understanding ultimate compounds which fit with biomolecular target is crucial to combat diseases and development of new class of effective drugs. In this review a concise evaluation of pharmacological activities of metabolites reported for O. sinensis are done to provide insight into its biologically active components.

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Heat and light stresses affect metabolite production in the fruit body of the medicinal mushroom Cordyceps militaris

Zhang

Wang

Liu

et al. 2018

Appl Microbiol Biotechnol

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Cordyceps militaris is a highly valued edible and medicinal fungus due to its production of various metabolites, including adenosine, cordycepin, N-(2-hydroxyethyl)-adenosine, and carotenoids. The contents of these metabolites are indicative of the quality of commercially available fruit body of this fungus. In this work, the effects of environmental abiotic factors, including heat and light stresses, on the fruit body growth and metabolite production in C. militaris were evaluated during the late growth stage. The optimal growth temperature of C. militaris was 20 °C. It was found that a heat stress of 25 °C for 5-20 days during the late growth stage significantly promoted cordycepin and carotenoid production without affecting the biological efficiency. Light stress at 6000 lx for 5-20 days during the late growth stage significantly promoted cordycepin production but decreased the carotenoid content. Both heat and light stresses promoted N-(2-hydroxyethyl)-adenosine production. In addition, gene expression analysis showed that there were simultaneous increases in the expression of genes encoding a metal-dependent phosphohydrolase (CCM_04437) and ATP phosphoribosyltransferase (CCM_04438) that are involved in the cordycepin biosynthesis pathway, which was consistent with the accumulation of cordycepin during heat stress for 5-20 days. A positive weak correlation between the cordycepin and adenosine contents was observed with a Pearson correlation coefficient of 0.338 (P < 0.05). The results presented herein provide a new strategy for the production of a superior quality fruit body of C. militaris and contribute to further elucidation of the effects of abiotic stress on metabolite accumulation in fungi.

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On the reliability of fungal materials used in studies on Ophiocordyceps sinensis

Cited by 35 publications

References 32 publications

Isolation, Culture and Characterization of Hirsutella sinensis Mycelium from Caterpillar Fungus Fruiting Body

Isolation, Culture and Characterization of Hirsutella sinensis Mycelium from Caterpillar Fungus Fruiting Body

Review on Pharmacologically Active Metabolites from Yarsagumba (Ophiocordyceps sinensis), an Epitome of Himalayan Elixir

Heat and light stresses affect metabolite production in the fruit body of the medicinal mushroom Cordyceps militaris

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