On the relation between surface roughness of metallic substrates and adhesion of human primary bone cells

Abstract

Surface characteristics of materials, whether their topography, chemistry, or surface energy, play an essential part in osteoblast adhesion on biomaterials. Thus, the quality of cell adhesion will influence the cell's capacity to proliferate and differentiate in contact with a biomaterial. We have developed for more than ten years numerous studies on the influence of topography and chemistry of metallic substrates on the response of primary human bone cells. The originality of our approach is that contrary to most of other authors, we quantified the adhesion of primary human bone cells on metallic substrates with perfectly characterized surface topography after some hours but also over 21 days. Moreover, we have developed original statistical approaches for characterizing the relation between surface roughness and cell-adhesion parameters. In this article, we will illustrate different studies we did these last ten years concerning the development of a new adhesion parameter, the adhesion power; the correlation between short-term adhesion, long-term adhesion, and proliferation; the influence of roughness organization on cell adhesion and the development of the order parameter; our modeling approach of cell adhesion on surface topography; the relative influence of surface chemistry and topography on cell adhesion and contact angle; the relation between surface features dimensions and cell adhesion. Further, some considerations will be given on the methods for scanning surface topography for cell-adhesion studies. Finally, perspectives will be given to elucidate these intracellular mechanotransduction mechanisms induced by the deformation of cells on model sinusoidal peaks-or-valleys surfaces.

Low‐pressure plasma surface modification of polyurethane films with chitosan and collagen biomolecules

“…Therefore, this film is not a suitable medium for the growth of fibroblast cells. The difference in the morphology of the cells cultivated at these surfaces can be explained by the fact that in terms of surface geometry, taller hills, and lower valleys on a rough surface, they may have more severe restrictions on the expansion of cells; therefore, the cells tend to extend along the grooves with lateral side extensions …”

Laser Surface Microstructuring of Biocompatible Materials Using a Microlens Array and the Talbot Effect: Evaluation of the Cell Adhesion

“…Several studies demonstrate the influence of the substrate roughness on the cellular adhesion [37,38,39], so the roughness of both materials was reduced by polishing to similar values before the microstructuring to minimize the influence of the initial roughness of the surface. Figure 4 shows SEM images of the tantalum surface before and after the polishing.…”

On the interactions of human bone cells with Ti6Al4V thermally oxidized by means of laser shock processing

“…That the treated alloy promotes cell colonization and proliferation was confirmed by immunostaining using a human FN antibody, which revealed that cells can reach confluence and organize a dense network of extracellular matrix. Polished Ti64 allows the osteogenic differentiation of hMSCs as well as the maturation of hOBs [2,8,20,40,41]. Differentiation of osteoprogenitor cells towards the osteoblastic phenotype was higher in the treated than on the untreated surface, as indicated by the measurements of ALP activity and matrix calcification of hMSCs cultured in media containing osteogenic inducers.…”