On the regeneration of aortic endothelium at different ages

Kunz, J; Keim, U

doi:10.1016/0047-6374(75)90036-6

Cited by 11 publications

(3 citation statements)

References 1 publication

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“…In general, the labeling index is less than 1% for aortic endothelium, although values up to 2.4% have been reported in areas prone to atherosclerosis (i.e., the aortic arch, bifurcations, and at the entrances of branch vessels) (Wright, 1987). With age, there is a tendency for these labeling indices to decline (Kunz and Keim, 1975;Schwartz and Bend, 1977). Creation of well-defined injuries in vivo has provided additional and valuable information on the modes and extent of endothelial repair.…”

Section: Discussionmentioning

confidence: 99%

Mitosis and cytokinesis in subconfluent endothelial cells exposed to increasing levels of shear stress

Wechezak

Viggers

Coan

et al. 1994

Journal Cellular Physiology

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An in vitro flow apparatus in combination with cultured endothelium was used to determine the effects of fluid-generated shear stress on cells undergoing mitosis and cytokinesis. Cell responses were recorded by time-lapse video microscopy under phase contrast or Hoffman modulation contrast optics. Completion of cell division in mitotic cells was dependent upon both the initial presence of intercellular attachments and the magnitude of fluid wall shear stress. In nonisolated populations, 95.3%, 69.5%, and 57.1% of the cells completed cell division as opposed to 66.6%, 20.4%, and 11.9% in the isolated cell groups at 2.8, 14.1, and 33 dynes/cm2, respectively. Prestressing cells for 14 h prior to monitoring failed to increase retention of isolated mitotic cells. The presence of neighboring cells facilitated replication by providing an anchoring attachment or a luminal surface for completion of division. Cell detachment most commonly occurred at the onset of cytokinesis when substrate contact areas were minimal and focal contacts were absent. A comparison between no flow controls and shear stress specimens indicated no significant differences in transit times for mitosis and cytokinesis. Thus, subconfluent endothelial cells may be more susceptible to detachment during cell division due to increases in shear stress, the absence of intercellular attachments, and reduced cell-substrate contacts.

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Section: Discussionmentioning

confidence: 99%

Mitosis and cytokinesis in subconfluent endothelial cells exposed to increasing levels of shear stress

Wechezak

Viggers

Coan

et al. 1994

Journal Cellular Physiology

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show abstract

“…Similar results have already been found for rabbit aortic endothelial cells. Kunz et al showed a significant decrease in the cell turnover with age, caused by both a lengthening of the cell cycle of the proliferating cells and a reduction of the growth fraction [39]. …”

Section: Discussionmentioning

confidence: 99%

TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties

Haddad

Gumez

Hawse

et al. 2011

Experimental Cell Research

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The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knockout tenocytes adhesion, spreading and proliferation were characterized on different substrates (fibronectin, collagen type I, gelatin and laminin) and the expression levels of various genes known to be involved with tendon development were analyzed by RT-PCR. The experiments revealed age-dependent and substrate-dependent properties for both wildtype and TIEG1 knockout tenocytes. Taken together, our results indicate an important role for TIEG1 in regulating tenocytes adhesion, spreading, and proliferation throughout the aging process. Understanding the basic mechanisms of TIEG1 in tenocytes may provide valuable information for treating multiple tendon disorders.

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“…Regenerating endothelial cells arise from sites adjacent to the injury and exhibit significant proliferative potential [67]. However, the regenerative response is significantly impaired with age in older subjects due to the development of endothelial senescence, which influences cell responses [68,69]. Endothelial progenitor cells (EPCs) mediate repair mechanisms for endothelial regeneration and maintenance, probably by directly differentiating into endothelial cells (ECs) and integrating into injured vessels, or act on cells and blood vessels by releasing paracrine substances [70].…”

Section: Endothelial Senescencementioning

confidence: 99%

Endothelial Senescence: From Macro- to Micro-Vasculature and Its Implications on Cardiovascular Health

Wang,

Konja,

Singh

et al. 2024

IJMS

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Endothelial cells line at the most inner layer of blood vessels. They act to control hemostasis, arterial tone/reactivity, wound healing, tissue oxygen, and nutrient supply. With age, endothelial cells become senescent, characterized by reduced regeneration capacity, inflammation, and abnormal secretory profile. Endothelial senescence represents one of the earliest features of arterial ageing and contributes to many age-related diseases. Compared to those in arteries and veins, endothelial cells of the microcirculation exhibit a greater extent of heterogeneity. Microcirculatory endothelial senescence leads to a declined capillary density, reduced angiogenic potentials, decreased blood flow, impaired barrier properties, and hypoperfusion in a tissue or organ-dependent manner. The heterogeneous phenotypes of microvascular endothelial cells in a particular vascular bed and across different tissues remain largely unknown. Accordingly, the mechanisms underlying macro- and micro-vascular endothelial senescence vary in different pathophysiological conditions, thus offering specific target(s) for therapeutic development of senolytic drugs.

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On the regeneration of aortic endothelium at different ages

Cited by 11 publications

References 1 publication

Mitosis and cytokinesis in subconfluent endothelial cells exposed to increasing levels of shear stress

Mitosis and cytokinesis in subconfluent endothelial cells exposed to increasing levels of shear stress

TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties

Endothelial Senescence: From Macro- to Micro-Vasculature and Its Implications on Cardiovascular Health

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