On the regeneration and repair of superficial wounds in the skin of &lt;i&gt;Rita vita&lt;/i&gt; (Ham.) (&lt;i&gt;Bagridae, Pisces&lt;/i&gt;)

Mittal, Ajay Kumar; Munshi, J S

doi:10.1159/000144250

Cited by 49 publications

(46 citation statements)

References 8 publications

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“…Some researchers have also developed a tissue culture system in which keratocytes rebuild or grow into epidermal sheets (Bereiter-Hahn et al 1979;Nolan et al 2002;Ream et al 2003;Sharma et al 2005). However, none of these are suitable in vitro models for epidermal re-epithelialization, because of the delayed onset or low reproducibility of the epidermal outgrowth compared with in vivo wound healing (Iger and Abraham 1990;Mittal and Munshi 1974;Quilhac and Sire 1999). In our culture system, since the isolated scale tissue has skin only on the posterior half and can be fixed onto a coverslip at the anterior portion without further distortion of the skin tissue, epidermal outgrowth is highly reproducible and is easily directed to expand over the coverslip by the addition of FBS.…”

Section: Discussionmentioning

confidence: 99%

Dermal matrix proteins initiate re-epithelialization but are not sufficient for coordinated epidermal outgrowth in a new fish skin culture model

Matsumoto

Sugimoto

2006

Cell Tissue Res

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We have established a new culture system to study re-epithelialization during fish epidermal wound healing. In this culture system, fetal bovine serum (FBS) stimulates the epidermal outgrowth of multi-cellular layers from scale skin mounted on a coverslip, even when cell proliferation is blocked. The rate of outgrowth is about 0.4 mm/h, and at 3 h after incubation, the area occupied by the epidermal sheet is nine times larger than the area of the original scale skin. Cells at the bottom of the outgrowth show a migratory phenotype with lamellipodia, and "purse string"-like actin bundles have been found over the leading-edge cells with polarized lamellipodia. In the superficial cells, re-development of adherens junctions and microridges has been detected, together with the appearance and translocation of phosphorylated p38 MAPK into nuclear areas. Thus, this culture system provides an excellent model to study the mechanisms of epidermal outgrowth accompanied by migration and re-differentiation. We have also examined the role of extracellular matrix proteins in the outgrowth. Type I collagen or fibronectin stimulates moderate outgrowth in the absence of FBS, but development of microridges and the distribution of phosphorylated p38 MAPK are attenuated in the superficial cells. In addition, the leading-edge cells do not have apparent "purse string"-like actin bundles. The outgrowth stimulated by FBS is inhibited by laminin. These results suggest that dermal substrates such as type I collagen and fibronectin are able to initiate epidermal outgrowth but require other factors to enhance such outgrowth, together with coordinated alterations in cellular phenotype.

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Section: Discussionmentioning

confidence: 99%

Dermal matrix proteins initiate re-epithelialization but are not sufficient for coordinated epidermal outgrowth in a new fish skin culture model

Matsumoto

Sugimoto

2006

Cell Tissue Res

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“…There exists a definite lymphatic plexus containing variable numbers of lymphocytes in the skin (Mittal and Munshi, 1971;Banerjee, 1974 a, b, 1975;Banerjee and Mittal, 1976;Mittal, Agarwal and Banerjee, 1976) which has been correlated with the local defence mechanism of the fish (Mittal and Munshi, 1971;1974). The discovery of lymphocytes lying in the clear areas even in the middle and outermost layer of the epidermis of H. fossilis is significant and may further increase the efficiency of its protective function.…”

Section: Discussionmentioning

confidence: 99%

Protein Histochemistry in the Skin of a Fresh-Water Catfish Heteropneustes Fossilis (Bloch) (Heteropneustidae, Pisces)

Mittal

1976

Acta Histochem. Cytochem

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“…In fact, the epithelialization of the wound gap in fish species involves changes in the surface architecture of the epithelial cells. As a quick response to injury, profuse mucous secretion and accumulation are observed on the surface of the adjacent epithelial cells, which is also associated with the protective function of mucus against pathogenic microorganisms [70][71][72]. Furthermore, a very noticeable property of teleost fishes is that they can fully regenerate largelysevered appendages with different tissues, as can several aquatic urodele amphibians.…”

Section: Teleost Skinmentioning

confidence: 99%

An Overview of the Immunological Defenses in Fish Skin

2012

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The vertebrate immune system is comprised of numerous distinct and interdependent components. Every component has its own inherent protective value, and the final combination of them is likely to be related to an animal's immunological history and evolutionary development. Vertebrate immune system consists of both systemic and mucosal immune compartments, but it is the mucosal immune system which protects the body from the first encounter of pathogens. According to anatomical location, the mucosa-associated lymphoid tissue, in teleost fish is subdivided into gut-, skin-, and gill-associated lymphoid tissue and most available studies focus on gut. The purpose of this paper is to summarise the current knowledge of the immunological defences present in skin mucosa as a very important part of the fish immune system, serving as an anatomical and physiological barrier against external hazards. Interest in defence mechanism of fish arises from a need to develop health management tools to support a growing finfish aquaculture industry, while at the same time addressing questions concerning origins and evolution of immunity in vertebrates. Increased knowledge of fish mucosal immune system will facilitate the development of novel vaccination strategies in fish.

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On the regeneration and repair of superficial wounds in the skin of <i>Rita vita</i> (Ham.) (<i>Bagridae, Pisces</i>)

Cited by 49 publications

References 8 publications

Dermal matrix proteins initiate re-epithelialization but are not sufficient for coordinated epidermal outgrowth in a new fish skin culture model

Dermal matrix proteins initiate re-epithelialization but are not sufficient for coordinated epidermal outgrowth in a new fish skin culture model

Protein Histochemistry in the Skin of a Fresh-Water Catfish Heteropneustes Fossilis (Bloch) (Heteropneustidae, Pisces)

An Overview of the Immunological Defenses in Fish Skin

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