On the quantitative interpretation of biomembrane structure by Raman spectroscopy

Gaber, Bruce P.; Peticolas, Warner L.

doi:10.1016/0005-2736(77)90078-5

Cited by 621 publications

(540 citation statements)

References 28 publications

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“…In fact, in lipids, the ratio of the symmetric methylene C-H stretching-mode intensities (peak height I2890/I2850) is sensitive to the packing order of the acyl chains and the observed relative increase of intensity of the 2850 cm -1 band with respect to 2890 cm -1 may account for a change of the lipids bilayer structure (Vyumvuhore et al, 2013). This phenomenon, occurring with naked AR, but not with coated TiO2 with similar titania core, is possibly due to physical interaction of nano-TiO2 with the SC lipids and/or to some TiO2-induced chemical oxidative damage (Gaber and Peticolas, 1977;Gniadecka et al, 1998 andWegener et al, 1996).…”

Section: Spectroscopic Investigation Of the Stretching Mode Of Lipidsmentioning

confidence: 97%

The influence of surface charge and photo-reactivity on skin-permeation enhancer property of nano-TiO2 in ex vivo pig skin model under indoor light

Peira

Turci

Corazzari

et al. 2014

International Journal of Pharmaceutics

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Several topical products contain nanometric TiO2 (nano-TiO2), which is an useful and safe component that absorbs UV light and does not cross skin barrier. However nano-TiO2 may impregnate the first layer of the skin (stratum corneum, SC) and generate free radicals, even under low UV irradiation. These properties, largely dependent on TiO2 surface chemistry, may modulate the transdermal drug permeation. To investigate how TiO2 surface properties affect drug permeation, Amphotericin in two different media, in the presence of three differently coated samples, was applied on skin and the flux measured. The naked, but not the coated, nano-TiO2 showed enhancer property, with a fourfold increase of the drug flux. Only the positively-charged naked TiO2 strongly adhered to and altered the SC structure. The oxidative potential towards formate anion and linoleic acid was assessed and a molecular mechanism to elucidate increased skin permeability proposed.To enhance the drug permeation, both a surface charge-driven adhesion and an oxidative disorganization of the SC lipids are required. By modulating TiO2 surface charge (coating) and its oxidative potential (crystalline phase), the enhancer effect of nano-TiO2 may be tuned and turned up or down when transdermal penetration of drug has to be favored or impaired.

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Section: Spectroscopic Investigation Of the Stretching Mode Of Lipidsmentioning

confidence: 97%

The influence of surface charge and photo-reactivity on skin-permeation enhancer property of nano-TiO2 in ex vivo pig skin model under indoor light

Peira

Turci

Corazzari

et al. 2014

International Journal of Pharmaceutics

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“…60 This spectral region also plays a fundamental role to reveal the phase behavior of other biological samples. 28,61,62 Changes of both the peak positions and the intensity ratio of trans to gauche structures affect the conformational state of acyl chains and the trans/gauche conformers population of lipids. 61 In fact, the population ratio of trans/gauche conformers changes considerably during the phase transitions, especially during the main transitions.…”

Section: Discussionmentioning

confidence: 99%

“…28,61,62 Changes of both the peak positions and the intensity ratio of trans to gauche structures affect the conformational state of acyl chains and the trans/gauche conformers population of lipids. 61 In fact, the population ratio of trans/gauche conformers changes considerably during the phase transitions, especially during the main transitions. 63 The relative number of gauche and trans conformers in lipids chains is indicated by the intensity ratios of m(CÀ ÀC) G ($1086 cm 21 ) to m(CÀ ÀC) T ($1063 cm 21 ) i.e., m(C2C) G /m(C2C) T .…”

Section: Discussionmentioning

confidence: 99%

Vibrational spectroscopic studies of newly developed synthetic biopolymers

2010

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Vibrational spectroscopic techniques such as near‐infrared (NIR), Fourier transform infrared (FTIR), and Raman spectroscopy are valuable diagnostic tools that can be used to elucidate comprehensive structural information of numerous biological samples. In this review article, we have highlighted the advantages of nanotechnology and biophotonics in conjunction with vibrational spectroscopic techniques in order to understand the various aspects of new kind of synthetic biopolymers termed as polyethylene glycol (PEG)ylated lipids. In contrast to conventional phospholipids, these novel lipids spontaneously form liposomes or nanovesicles upon hydration, without the supply of external activation energy. The amphiphiles considered in this study differ in their hydrophobic acyl chain length and contain different units of PEG hydrophilic headgroups. We have further explored the thermotropic phase behaviors and associated changes in the conformational order/disorder of such lipids by using variable‐temperature FTIR and Raman spectroscopy. Phase transition temperature profiles and correlation between various spectral indicators have been identified by either monitoring the shifts in the vibrational peak positions or plotting vibrational peak intensity ratios in the CH stretching region as a function of temperature. To supplement our observations of phase transformations, a thermodynamic approach known as differential scanning calorimetry (DSC) has been applied and revealed a good agreement with the infrared and Raman spectroscopic data. Finally, the investigation of thermal properties of lipids is extremely crucial for numerous purposes, thus the results obtained in this work may find application in a wide variety of studies including the development of PEGylated lipid based drug and substances delivery vehicles. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 403–417, 2010.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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“…Raman spectroscopy experiments, coupled to normal coordinate analysis, allow the use of the relative intensity of the band assigned to the totally symmetric C-C stretching mode, as an order-parameter which gives a quantitative measure of the trans to gauche orientations within those systems [1][2][3]. Moreover, in order to study the environment interactions-both lateral packing and top-to-end-special attention should be paid to the changes observed in the C-H stretching region.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, in order to study the environment interactions-both lateral packing and top-to-end-special attention should be paid to the changes observed in the C-H stretching region. In fact, for hydrocarbon chains it has been observed that while the band due to the methylene C-H antisymmetric mode broadens, loses intensity and shifts to higher frequencies, as the intermolecular lateral interactions decrease, the symmetric stretching one remains sharp as well as the most intense feature in the spectrum [2,4]. A quantitative measure of this lateral order was thus developed [2], as a function of the relative observed intensity of the C-H symmetric and antisymmetric stretching bands.…”

Section: Introductionmentioning

confidence: 99%

Intra- versus interchain interactions in α,ω-polyamines: a Raman spectroscopy study

Costa

Marques

Carvalho

2004

Vibrational Spectroscopy

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Raman spectroscopy is used to examine the intra-and interchain interactions in aliphatic diamines H 2 N (CH 2 ) n NH 2 (n ¼ 2-10 and 12) and their N-deuterated derivatives, either as a function of chainlength or as a function of temperature. The intensity of the in-phase C-C stretching mode divided by the integrated area of the N-H or the N-D stretching regions is discussed as a parameter which can be used as a quantitative measure of the average number of trans bonds in the hydrocarbon chain, while the relative intensity of the C-H antisymmetric and symmetric stretching vibrations is used as a measure of the lateral packing effects of the hydrocarbon chains. #

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On the quantitative interpretation of biomembrane structure by Raman spectroscopy

Cited by 621 publications

References 28 publications

The influence of surface charge and photo-reactivity on skin-permeation enhancer property of nano-TiO2 in ex vivo pig skin model under indoor light

The influence of surface charge and photo-reactivity on skin-permeation enhancer property of nano-TiO2 in ex vivo pig skin model under indoor light

Vibrational spectroscopic studies of newly developed synthetic biopolymers

Intra- versus interchain interactions in α,ω-polyamines: a Raman spectroscopy study

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