On the preparation of secretin and pancreozymin

Crick, Joan; Harper, A. A.; Raper, Henry Stanley

doi:10.1113/jphysiol.1949.sp004445

Cited by 117 publications

(61 citation statements)

References 7 publications

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“…Enzyme secretion was stimulated by rapid pulses or prolonged infusions of acetylcholine chloride or, less frequently, of cholecystokinin-pancreozymin (CCK-Pz). Secretin and CCK-Pz were prepared by the method of Crick, Harper & Raper (1949), though in some experiments purer preparations (G.I.H. Laboratory, Karolinska Institutet, Stockholm) were used.…”

Section: Methodsmentioning

confidence: 99%

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Argent¹,

Case²,

Scratcherd³

1973

The Journal of Physiology

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Section: Methodsmentioning

confidence: 99%

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Argent¹,

Case²,

Scratcherd³

1973

The Journal of Physiology

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“…Secretin was prepared by the method of Crick, Harper & Raper (1949) Samples of pancreatic juice were collected in tared tubes, weighed and stored overnight at 4 'C. Enzyme secretion was monitored by measuring amylase activity using the method of N0rby (Lagerlof, 1942).…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin action on pancreatic blood flow and on electrolyte and enzyme secretion by exocrine pancreas in vivo and in vitro

Case¹,

Scratcherd²

1972

The Journal of Physiology

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SUMMARY1. Intra-arterial injection or infusion of prostaglandins E1 and E2 into anaesthetized cats caused a fall in arterial blood pressure, a reduction in pancreatic blood flow and an inhibition of secretin-stimulated pancreatic electrolyte secretion. In some experiments these effects were preceded by a transient increase in blood flow and secretion.2. The fall in blood pressure and reduction in blood flow, but not the inhibition of secretion, were much less marked following administration of the a-adrenergic blocking agent phenoxybenzamine. This stimulatory action was markedly potentiated by theophylline. 5. Enzyme secretion was not stimulated by any of the prostaglandins, even in the presence of theophylline.6. It is concluded that prostaglandins can stimulate electrolyte transport by exocrine pancreas, perhaps through a mechanism involving adenylate cyclase, but that in vivo this action is masked by a secondary inhibition resulting either from vasoconstriction, or from the vibration of an antisecretory agent, or both.

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“…The concentration of Pz is expressed in Crick, Harper & Raper (1950) Electrical recording Intracellular recordings were made from the pancreatic acinar cells on the surface of the pancreas by manually advancing KCl-filled micro-electrodes under direct visual control, and the potentials were displayed on a direct visual oscillograph (Yokogawa, Type 2915, Japan). The Wheatstone bridge circuit was used to pass current of 50 msec in duration through the recording electrode in order to polarize the cell membrane.…”

Section: Isolation and Perfusion Of The Pancreasmentioning

confidence: 99%

The electrogenic sodium pump in the hyperpolarizing and secretory effects of pancreozymin in the pancreatic acinar cell.

Kanno¹

1975

The Journal of Physiology

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SUMMARY1. Transmembrane potential, effective membrane resistance, and amylase release were recorded simultaneously from acinar cells of isolated rat pancreas perfused with Krebs-Henseleit solution.2. The hyperpolarizing effect of pancreozymin (Pz) was confirmed by perfusing with a solution containing 5 m-u. Pz/ml.3. The hyperpolarizing effect of Pz disappeared in the following environments: (a) in low ambient temperature, (b) in K-free medium, (c) in low Na medium, and (d) in the presence of ouabain. In these environments, transient depolarization was frequently observed immediately after stimulation.It is suggested that there are two components in the effect of Pz on the membrane potential of the acinar cells: transient depolarization which coincides with an increase in Na permeability in the initial phase, and continuous hyperpolarization due to an electrogenic Na pump which conceals transient depolarizing phase when the pump is dominant.4. The secretary effect of Pz was inhibited under conditions that suppressed the electrogenic Na pump.It is proposed that the Na pump activated by Pz maintains the passive Na-influx, increases [Ca2+]I, and, in consequence, upholds the amylase output during continuous stimulation. 5. A medium which was used to bathe the sectioned pancreas with Pz was found to contain a substance which depolarized the acinar cells.

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On the preparation of secretin and pancreozymin

Cited by 117 publications

References 7 publications

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Amylase secretion by the perfused cat pancreas in relation to the secretion of calcium and other electrolytes and as influenced by the extrenal ionic environment*

Prostaglandin action on pancreatic blood flow and on electrolyte and enzyme secretion by exocrine pancreas in vivo and in vitro

The electrogenic sodium pump in the hyperpolarizing and secretory effects of pancreozymin in the pancreatic acinar cell.

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