On the oligomeric state of chloroplast chaperonin 10 and chaperonin 20

Sharkia, Rajech; Bonshtien, Anat L.; Mizrahi, Itzhak; Weiss, Celeste; Niv, Adina; Lustig, Ariel; Viitanen, Paul V.; Azem, Abdussalam

doi:10.1016/s1570-9639(03)00237-1

Cited by 27 publications

(29 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chloroplastic elongation factor Tu (EF-Tu) (gi|449529128) and the mitochondrial elongation factor Tu (EF-Tu) (gi|449515049), which function in elongation of the growing peptide chains (Momcilovic and Ristic 2007;Suzuki et al 2007), were down-regulated under salt stress, but were induced by NaCl applied with Spd. Exogenous Spd also induced three proteins that decreased abundances of proteins under salt stress, a 10 kDa chaperonin (gi|449470662), a 20 kDa chaperonin (gi|449521557) and a trigger factor-like protein TIG (gi|449463242), which function in chloroplast protein folding (Geisler and Bailly 2007;Sharkia et al 2003). These results suggested that high level of polyamines might assist their molecular partners and play roles in protein synthesis, processing and assembly.…”

Section: Proteins Involved In Protein Synthesis Folding Transportatmentioning

confidence: 93%

Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings

Shen

et al. 2016

Plant Cell Rep

View full text Add to dashboard Cite

Our results based on proteomics data and physiological alterations proposed the putative mechanism of exogenous Spd enhanced salinity tolerance in cucumber seedlings. Current studies showed that exogenous spermidine (Spd) could alleviate harmful effects of salinity. It is important to increase our understanding of the beneficial physiological responses of exogenous Spd treatment, and to determine the molecular responses underlying these responses. Here, we combined a physiological analysis with iTRAQ-based comparative proteomics of cucumber (Cucumis sativus L.) leaves, treated with 0.1 mM exogenous Spd, 75 mM NaCl and/or exogenous Spd. A total of 221 differentially expressed proteins were found and involved in 30 metabolic pathways, such as photosynthesis, carbohydrate metabolism, amino acid metabolism, stress response, signal transduction and antioxidant. Based on functional classification of the differentially expressed proteins and the physiological responses, we found cucumber seedlings treated with Spd under salt stress had higher photosynthesis efficiency, upregulated tetrapyrrole synthesis, stronger ROS scavenging ability and more protein biosynthesis activity than NaCl treatment, suggesting that these pathways may promote salt tolerance under high salinity. This study provided insights into how exogenous Spd protects photosynthesis and enhances salt tolerance in cucumber seedlings.

show abstract

Section: Proteins Involved In Protein Synthesis Folding Transportatmentioning

confidence: 93%

Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings

Shen

et al. 2016

Plant Cell Rep

View full text Add to dashboard Cite

show abstract

“…CPN20 is part of the family of Type I chaperonins that function in protein folding together with CPN60 proteins and CPN10 proteins (Koumoto et al, 1999;Sharkia et al, 2003;Vitlin Gruber et al, 2013). It remains to be determined if and how CPN20 has a functional relationship to ClpT1 and ClpT2.…”

Section: Interactions Between Clpt and Cpn20mentioning

confidence: 99%

Structures, Functions, and Interactions of ClpT1 and ClpT2 in the Clp Protease System of Arabidopsis Chloroplasts

et al. 2015

View full text Add to dashboard Cite

“…Chloroplasts contain two dominant protein chaperones systems, namely the CPN60/CPN21/CPN10 (66,67) and the HSP70/GrpE system (68). The Arabidopsis HSP70-1 (At4g24280.1) and HSP70-2 (At5g49910.1) are abundant proteins with a broad substrate pool, and their abundance was constant across all the HM-A fractions, consistent with their wide range of substrates.…”

Section: Comparison Of Hm and Lm Data Sets Reveals Effective Sec Fracmentioning

confidence: 99%

Megadalton Complexes in the Chloroplast Stroma of Arabidopsis thaliana Characterized by Size Exclusion Chromatography, Mass Spectrometry, and Hierarchical Clustering

Olinares

Ponnala

Wijk

2010

Molecular & Cellular Proteomics

178

183

View full text Add to dashboard Cite

To characterize MDa-sized macromolecular chloroplast stroma protein assemblies and to extend coverage of the chloroplast stroma proteome, we fractionated soluble chloroplast stroma in the non-denatured state by size exclusion chromatography with a size separation range up to ϳ5 MDa. To maximize protein complex stability and resolution of megadalton complexes, ionic strength and composition were optimized. Subsequent high accuracy tandem mass spectrometry analysis (LTQ-Orbitrap) identified 1081 proteins across the complete native mass range. Protein complexes and assembly states above 0.8 MDa were resolved using hierarchical clustering, and protein heat maps were generated from normalized protein spectral counts for each of the size exclusion chromatography fractions; this complemented previous analysis of stromal complexes up to 0.

show abstract

On the oligomeric state of chloroplast chaperonin 10 and chaperonin 20

Cited by 27 publications

References 24 publications

Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings

Comparative proteomic analysis reveals the positive effect of exogenous spermidine on photosynthesis and salinity tolerance in cucumber seedlings

Structures, Functions, and Interactions of ClpT1 and ClpT2 in the Clp Protease System of Arabidopsis Chloroplasts

Megadalton Complexes in the Chloroplast Stroma of Arabidopsis thaliana Characterized by Size Exclusion Chromatography, Mass Spectrometry, and Hierarchical Clustering

Contact Info

Product

Resources

About