On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters

Pekmezci, Gökmen Zafer; Yardımcı, Banu

doi:10.1007/s00436-019-06278-x

Cited by 13 publications

(8 citation statements)

References 52 publications

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“…The present species formed a well-supported clade with the sister group C. gibsoni Mattiucci, Paoletti, Solorzano and Nascetti, 2010 and C. overstreeti Mattiucci, Paoletti, Solorzano and Nascetti, 2010, but it is genetically and morphologically distinct. The sequences of C. gibsoni (EU852340) and C. overstreeti (EU852348) were obtained from adult worms recovered from Pelecanus crispus (L.) in Greece and C. overstreeti (MG495095) was obtained from larvae infecting Mugil cephalus in Turkey (Mattiucci et al , 2010; Pekmezci & Yardimci, 2019). The adults of C. gibsoni and C. overstreeti are larger than C. jorgei n. sp.…”

Section: Discussionmentioning

confidence: 99%

A new species ofContracaecum(Nematoda: Anisakidae) found parasitizingNannopterum brasilianus(Suliformes: Phalacrocoracidae) andHoplias argentinensis(Characiformes: Erythrinidae) in South America: morphological and molecular characterization of larval and adult stages

et al. 2020

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Nematode species of the genus Contracaecum Railliet & Henry, 1912 have been reported around the world in many species of fish-eating birds and seals. Here, Contracaecum jorgei n. sp. is morphologically described using light and scanning electron microscopy for adults and fourth-stage larvae (L4) found in the bird Nannopterum brasilianus and third-stage larvae (L3) found in the freshwater fish Hoplias argentinensis, both from the province of Córdoba, Argentina. Additionally, sequences of cytochrome c oxidase subunit II were obtained from these specimens and molecular phylogenetic analysis was used to determine its relationships within the genus. The present species is distinguished from other species by the number and disposition of cephalic papillae; shape and size of the interlabia; length of the spicules; and number and arrangement of papillae in the posterior end of the male. Furthermore, in the molecular analyses, sequences obtained from adult L4 and L3 specimens of C. jorgei n. sp. were similar and grouped, forming an independent lineage, thus confirming it as a distinct species. Thus, morphological characteristics associated with molecular data support the proposal of a new species.

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Section: Discussionmentioning

confidence: 99%

A new species ofContracaecum(Nematoda: Anisakidae) found parasitizingNannopterum brasilianus(Suliformes: Phalacrocoracidae) andHoplias argentinensis(Characiformes: Erythrinidae) in South America: morphological and molecular characterization of larval and adult stages

et al. 2020

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“…2018 ; Malviya et al ., 2018 ). There are a few publications on the specific identification of the larval stages of Contracaecum in fishes from the world ( Szostakowska & Fagerholm, 2007 ; Shamsi & Aghazadeh-Meshgi, 2011 ; Shamsi et al ., 2017 ; Molnár et al ., 2019 ; Pekmezci & Yardimci, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Ultramorphology and molecular studies of Contracaecum larvae (Nematoda: Anisakidae) collected in five Cyprinid fish species from Sulaimani Province, Kurdistan Region-Iraq

Abdullah

Hussein

2021

Helminthologia

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SummaryA total of 1134 freshwater fishes belonging to Cyprinidae (Acanthobrama marmid (n=20), Alburnus caeruleus (n=7), Alburnus mossulensis (n=62), Arabibarbus grypus (n=123), Barbus lacerta (n=7), Capoeta trutta (n=222), C. umbla (n=161), Carasobarbus kosswigi (n=5), C. luteus (n=89), Carassius auratus (n=54), Chondrostoma regium (n=52), Cyprinion kais (n=10) and C. macrostomum (n=322)) were collected in different water bodies in Sulaimani Province, Kurdistan Region-Iraq for the presence of larval nematode of the genus Conteacaecum. This investigation revealed that 17 fishes belonged to five species (A. marmid, A. grypus, C. trutta, C. luteus and C. regium) were infected with Contracaecum larvae with prevalence of 35 %, 0.81 %, 0.90 %, 4.49 % and 5.76 %, respectively. The third- larval stage was morphologically studied by optical microscopy, and the ultrastructure was investigated using scanning electron microscopy (SEM). In addition, molecular analysis was carried out by amplifying, sequencing and comparing different gene loci, including internal transcribed spacers (ITS-1 and ITS-2) and cytochrome oxidase c subunit-II (COX-2), of the different isolated Contracaecum larvae. These sequences were also compared with closely related nematode sequences from the GenBank. Fifteen sequences were obtained for this study from the collected Contracaecum larvae. ITS-1, ITS-2 and COX-2 were amplified by polymerase chain reaction (PCR) and sequenced. The sequences of ITS-1, ITS-2 and COX-2 revealed that the collected Contracaecum larval specimens from all infected fish species represented one species (Contracaecum rudolphii B) based on the identity percentage in the GenBank database. The genetic characterisation of the parasite in the present study is available in the GenBank database, and the obtained ITS-1, ITS-2 and COX-2 sequences were deposited in GenBank. The present study provides information on the accurate identification and molecular analysis of Contracaecum larvae in the infected fish species in Sulaimani Province, Kurdistan Region-Iraq.

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“…Current investigation provides the first molecular data of the rrnS gene of Anisakis pegreffii from the Turkish marine waters. To date, among ascaridoid nematodes, Hysterothylacium aduncum, H. fabri, A. typica, and Contracaecum overstreeti species from Turkish marine waters have been molecularly characterized based on mitochondrial rrnS gene (Pekmezci, 2019;Pekmezci & Yardimci, 2019;Pekmezci & Onuk, 2020;Simsek et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Both mitochondrial cox2 and nuclear ITS data of Anisakis species from marine fish were previously reported from Turkish marine waters (Pekmezci et al, 2014). Moreover, genetic analyses of rrnS loci of Hysterothylacium aduncum, H. fabri, Contracaecum overstreeti, and A. typica among ascaridoids nematodes of marine fish were molecularly made from the coast of Turkey (Pekmezci, 2019;Pekmezci & Yardimci, 2019;Pekmezci & Onuk, 2020;Simsek et al, 2021). There is no study about the genetic characterization of the mitochondrial rrnS gene of A. pegreffii from the Turkish marine waters.…”

Section: Introductionmentioning

confidence: 96%

Genetic Analysis of the Mitochondrial rrnS Gene of Zoonotic Anisakis pegreffii (Nematoda: Anisakidae) Isolated from Micromesistius Poutassou (R.) in the Aegean Sea

Pekmezci

2021

Journal of Anatolian Environmental and Animal Sciences

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Ascaridoid nematodes were found in viscera of the blue whiting Micromesistius poutassou (Risso, 1826) from the Aegean Sea in the parasitological examination. Collected nematodes were morphologically identified as the third (L3) stage of Anisakis type I larvae and then subject to Restriction Fragment Length Polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region to identify the species. Randomly selected larvae were molecularly confirmed as Anisakis pegreffii by RFLP analysis. Subsequently, L3 of A. pegreffii were amplified and bi-directionally sequenced at the small subunit ribosomal RNA (rrnS) gene. The rrnS sequence of A. pegreffii (MT484284) had 100% identity with the rrnS gene of A. pegreffii (JX500050, LC222461, MF140359, MT312511, MT312512) which were found in fish and cetaceans hosts, Scomber japonicus, Conger myriaster, Neophocaena asiaeorientalis, and Stenella coeruleoalba, from the Mediterranean Sea, Japanese, Chinese, and Korean waters, respectively. This study provided the genetic analysis of zoonotic A. pegreffii from the Turkish marine waters based on the rrnS gene for the first time. This sequence (MT484284) can be used as the novel rrnS sequence of A. pegreffii in the genetic analysis for ascaridoid nematodes in the Mediterranean Sea.

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On the occurrence and molecular identification of Contracaecum larvae (Nematoda: Anisakidae) in Mugil cephalus from Turkish waters

Cited by 13 publications

References 52 publications

A new species ofContracaecum(Nematoda: Anisakidae) found parasitizingNannopterum brasilianus(Suliformes: Phalacrocoracidae) andHoplias argentinensis(Characiformes: Erythrinidae) in South America: morphological and molecular characterization of larval and adult stages

A new species ofContracaecum(Nematoda: Anisakidae) found parasitizingNannopterum brasilianus(Suliformes: Phalacrocoracidae) andHoplias argentinensis(Characiformes: Erythrinidae) in South America: morphological and molecular characterization of larval and adult stages

Ultramorphology and molecular studies of Contracaecum larvae (Nematoda: Anisakidae) collected in five Cyprinid fish species from Sulaimani Province, Kurdistan Region-Iraq

Genetic Analysis of the Mitochondrial rrnS Gene of Zoonotic Anisakis pegreffii (Nematoda: Anisakidae) Isolated from Micromesistius Poutassou (R.) in the Aegean Sea

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