On the nature of folate derivatives in neurospora crassa and the effect of sulphanilamide

Vora, M. R.; Tamhane, D. V.

doi:10.1007/bf00446671

Cited by 7 publications

(9 citation statements)

References 20 publications

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“…The differential assays indicated that these two components are conjugates of a formylated tetrahydrofolate and of 5-methyl-H4PteGlu. Folates with similar chromatographic properties have been reported previously (5,32).…”

Section: Figsupporting

confidence: 79%

“…This is characteristic of conjugates of 5-methylH4PteGlu as well as formylated folate conjugates oxidized in the pteridine ring. Donaldson and Keresztesy (5) have reported a conjugate of 5-methyl-H4PteGlu at this location in the elution sequence, and an oxidized formyl folate conjugate may have been present at this location in folate profiles of Ne~lrospora crassa Shear and Dodge (32).…”

Section: Figmentioning

confidence: 95%

“…Tentative identification of the components in each peak fraction relied mainly on the differential response obtained with the assay organisms. Chromatographic data have been reported for various folate derivatives (1,5,25,32,33) and they were also used as a guide in identification. They are, however, of limited value for this purpose because of uncertainties regarding the degree of conjugation.…”

Section: Fractionation Of Folates By Deae-cellulosementioning

confidence: 99%

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Folate derivatives in ungerminated and germinated uredospores of wheat stem rust

Jackson¹,

Samborski²,

Rohringer³

et al. 1970

Can. J. Bot.

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1970. Folate derivatives in ungerminated and germinated uredospores of wheat stem rust. Can. J. Bot. 48: 1617-1623.Extracts of ungemlinated uredospores and of uredospores germinated for 6 h and 12 h were assayed for folates with Lactobncill~ts casei, Sfrepfococcus faecalis, and Pediococc~ls cerevisiae. These organisms did not respond to the extracts without conjugase treatment, indicating that most of the folates were present in conjugated form with more than three glutamic acid moieties per molecule. During the 6-h and 12-h gemination periods the content of growth factors for L. casei declined to 70.9y0 and 46.0y0 of initial levels. In this same period, the content of growth factors for S . faecnlis declined to 54.5% and 15.2% of initial levels, indicating an increase in the proportion of methylated folates during uredospore germinat~on.This trend was confirmed in a detailed analysis of folate components after fractionation of the extracts on DEAE-cellulose columns. The folate profiles consisted of five peaks. Two peak fractions present in profiles from ungerminated spores contained mostly formylated folates and were greatly reduced or absent in profiles from germinated spores. A peak fraction composed of a 5-methyl-tetrahydrofolate conjugate was not observed in profiles of ungerminated spores but was predominant in those from spores germinated for 12 h.

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Section: Figsupporting

confidence: 79%

Section: Figmentioning

confidence: 95%

Section: Fractionation Of Folates By Deae-cellulosementioning

confidence: 99%

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Folate derivatives in ungerminated and germinated uredospores of wheat stem rust

Jackson¹,

Samborski²,

Rohringer³

et al. 1970

Can. J. Bot.

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“…First, the effects on growing cell by inhibi tors, chloramphenicol (7-9) and sulfanilamide (10)(11)(12), were examined. Chloramphenicol and sulfanilamide scarcely affected the pH values even when added in high concentration.…”

Section: Discussionmentioning

confidence: 99%

“…It was found that xanthine is the most flavinogenic purine among various purines during these experiments. It has been well known that chlorampheni col (7-9) is a specific inhibitor of the protein biosynthesis and sulfanilamide (10)(11)(12) is an inhibitor of purine de novo synthesis. In this section, effects of these inhibitors on growth and riboflavin formation were examined in growing cells as preliminary experiments in order to elucidate the physiological conditions of non-growing cells.…”

mentioning

confidence: 99%

Development of Specific Experimental Systems for Flavinogenesis Using Non-Growing Cell of Eremothecium ashbyii

Mitsuda

Nakajima

1972

J. Vitaminol

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Experimental systems using non-growing cell of Eremothecium ashbyii to elucidate the mechanism of flavinogenesis were established in this paper.1. It was found that vacuum infiltration of purines for 2, 3 and 5min and moderate shaking of non-growing cell brought about a good reproducibility of the stimulatory effects on flavinogenesis by purines and furthermore, a higher flavinogenesis.2. The addition of an inhibitor of protein synthesis, chloramphenicol, to the basal medium at the concentration of 6mM after 1day of cultivation resulted in a 45.1% inhibition of the growth measured after 2days of cultivation and a 65.1% inhibition of riboflavin production measured after 4 days of cultivation. On the other hand, the addition of an inhibitor of purine de novo synthesis, sulfanilamide at 4mM resulted in a 24.3% inhibition of the growth after 2 days and a 44.1% inhibition of riboflavin formation after 4days respectively.3. The addition of these drugs and another inhibitor of protein synthesis, cycloheximide to the non-growing cell medium brought about little effects on flavinogenesis and only cycloheximide showed a 25% inhibition of riboflavin production at 7mM.4. It was elucidated from above results that non-growing cell under such the special conditions shows little or no synthesis of protein and purine derivatives but there occurs flavinogenesis to a fair extent.5. It was found that xanthine is the most flavinogenic purine among various purines during these experiments.

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Metabolic studies with neurospora: Action of 5-bromo- and 5-nitrouracils

Vora

Tamhane

1966

Archiv. Mikrobiol.

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On the nature of folate derivatives in neurospora crassa and the effect of sulphanilamide

Cited by 7 publications

References 20 publications

Folate derivatives in ungerminated and germinated uredospores of wheat stem rust

Folate derivatives in ungerminated and germinated uredospores of wheat stem rust

Development of Specific Experimental Systems for Flavinogenesis Using Non-Growing Cell of Eremothecium ashbyii

Metabolic studies with neurospora: Action of 5-bromo- and 5-nitrouracils

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