On the morphometric identity of populations of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 (Tylenchida: Hoplolaimidae)

Fortuner, Renaud; Louis, Pierre‐Yves; Geniet, Dominique

doi:10.1163/15685411-00003148

Cited by 13 publications

(19 citation statements)

References 16 publications

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“…Some culture methods for plant-parasitic nematodes have been successfully employed, but there have been great differences in culture techniques. For example, Aphelenchoides besseyi, Ditylenchus destructor and Bursaphelenchus xylophilus can be cultured on certain fungi [31][32][33]. A. ritzemabosi and D. dipsaci can reproduce rapidly on alfalfa tissue [19].…”

Section: Discussionmentioning

confidence: 99%

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

Xia

et al. 2022

Preprint

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Background: Spiral nematodes of the genus Helicotylenchus are globally distributed, and contain various species complexes that normally exhibit similar diagnostic characteristics. Some studies on the parasitism of tomato by spiral nematodes include only census data, or morphological data and lack detailed molecular data in China. At present, carrot disks have been successfully applied in the culture of plant-parasitic nematodes, but carrot disks are not suitable for rearing all migratory plant-parasitic nematodes, and only a small number of plant-parasitic nematodes can be cultured. However, the establishment of a culture method for Helicotylenchus has not been reported.Results: Spiral nematodes were recovered from tomato roots and soil samples, and the purified spiral nematode LQ-1 population was identified based on morphological and molecular biological technology. Both the morphological characteristics and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA sequences revealed that the species of the spiral nematode LQ-1 population was Helicotylenchus microlobus. Phylogenetic analysis with the rRNA sequences of the ITS and 28S D2-D3 regions was consistent with molecular identification, suggesting that the spiral nematode LQ-1 population formed a highly supported clade with other H. microlobus populations. Additionally, a method for culture of H. microlobus on carrot disks was established, and the effect of temperature on the reproduction rate (Rr) of this nematode was investigated. The optimum temperature for H. microlobus culture on carrot disks was 27.5 °C, and the Rr reached 406 after 90 days of inoculation with 30 females.Conclusions: To our knowledge, this is the first systematic description of H. microlobus from tomato in China. This study also demonstrated for the first time that the carrot disk method is suitable for the culture of H. microlobus. This study lays a foundation for other related research on H. microlobus, and has significance for the study of spiral nematodes.

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Section: Discussionmentioning

confidence: 99%

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

Xia

et al. 2022

Preprint

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“…The sequences obtained for H. californicus grouped with an isolate of Helicotylenchus from Calathea not identified to the species level and labeled as CD761 in Subbotin et al (2015) ( Table 7 ). Recently, Fortuner et al (2018) identified isolate CD761 as H. pseudorobustus using morphological and morphometric analysis. However, our populations are similar to preliminary reports of H. californicus ( Van Den Berg and Heyns, 1975 ; Krall, 1990 ).…”

Section: Discussionmentioning

confidence: 99%

An integrative approach to the study of Helicotylenchus (Nematoda: Hoplolaimidae) Colombian and Brazilian populations associated with Musa crops

Riascos-Ortiz

Mosquera-Espinosa

Agudelo

et al. 2020

Journal of Nematology

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In total, 10 populations of Helicotylenchus associated with Musa spp., six from Colombia, and four from Brazil were identified to the species level. Morphological and morphometric data were recorded for each population, performed a principal components analysis (PCA), a conglomerate, along with consensus sequences of D2 to D3 expansion segment of the large subunit of ribosomal DNA (28S) for different populations. Identify of species were performed using the basic local alignment search tool (BLAST), and the evolutionary relationships of species were explored using a phylogeny study. Four species of Helicotylenchus were identified based on morphological and morphometric data: H. multicinctus, H. dihystera, H. erythrinae, and H. californicus. PCA and conglomerate analysis clearly separated these species. BLAST and phylogenetic analysis confirmed the presence of these species associated with Musa spp. crops. This is the first report of these species in Colombia through integrative taxonomy.

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“…The regulated status of Ditylenchus spurred widespread research, which led to the discovery of numerous new species without giving adequate consideration to the limits of species variability [27]. Several taxonomists have reviewed the genus characteristics in attempts to limit the number of valid species by revisions and synonymization [3,27,28]. More recently, Hashemi and Karegar [16] updated the entire genus Ditylenchus and documented 60 nominal species; presently, the genus contains 63 species.…”

Section: Introductionmentioning

confidence: 99%

“…Further morphological comparisons revealed their identity as D. anchilisposomus (Tarjan) Fortuner [28,40], D. clarus Thorne and Malek [31], D. tenuidens Gritsenko [41], and D. valveus. All of these species are new records in Canada except for the D. valveus.…”

Section: Introductionmentioning

confidence: 99%

New Records of Ditylenchus Species from Southern Alberta, Canada

Maria

Rahman

Castillo

et al. 2023

Plants

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The presence of plant-parasitic nematodes (PPNs) in cultivated areas is a limiting factor in achieving marketable crop yield. To control and alleviate the effects of these nematodes and determine appropriate management strategies, species-level identification is crucial. Therefore, we conducted a nematode diversity survey, which resulted in the detection of four Ditylenchus species in cultivated areas of southern Alberta, Canada. The recovered species had six lines in the lateral field, delicate stylets (>10 µm long), distinct postvulval uterine sacs, and pointed to rounded tail tips. The morphological and molecular characterization of these nematodes revealed their identity as D. anchilisposomus, D. clarus, D. tenuidens and D. valveus, all of which are members of the D. triformis group. All of the identified species were found to be new records in Canada except for D. valveus. Accurate Ditylenchus species identification is crucial because false-positive identification can result in the implementation of quarantine measures over the detected area. Our current study not only documented the presence of Ditylenchus species from southern Alberta, but also described their morpho-molecular characteristics and subsequent phylogenetic relationships with related species. The results of our study will aid in the decision on whether these species should become a part of nematode management programs since nontarget species can become pests due to changes in cropping patterns or climate.

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On the morphometric identity of populations of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 (Tylenchida: Hoplolaimidae)

Cited by 13 publications

References 16 publications

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

An integrative approach to the study of Helicotylenchus (Nematoda: Hoplolaimidae) Colombian and Brazilian populations associated with Musa crops

New Records of Ditylenchus Species from Southern Alberta, Canada

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