On the merits of viable-cell immobilisation

Immobilized lactic acid bacteria for application as dairy starters and probiotic preparations

“…As noted by Dervakos and Webb (1991), many claims have been made for potential advantageous effect of immobilization on bioprocess performance, but these remain very much dependent on the choice of operating conditions and additional research is required for substantiations. Additional research should also be carried out to characterize the biological stability (stability of the populations, resistance to bacterial contamination, and biological evolution of the strains) of the mixed strain continuous entrapped cell fermentation over extended periods of operation.…”

Diffusion and Inhibition Processes in a Hollow-fiber Membrane Bioreactor for Hybridoma Culture. Development of a Mathematical Model

“…Cell confinement makes hybridomas grow, forming biofilms and conglomerates. This different environment interacts with cell development, altering morphology and membrane permeability, as well as surface tension and osmotic pressure 31,35,39 . Maximum specific rates found in this work were slightly higher than lactate and ammonium specific production rates and glucose specific consumption rate reported by Ozturk and Palsson 40 (0.13, 0.002, and 0.14 h -1 , respectively).…”

“…Another possibility is that lactate and/or ammonium inhibit antibody production. In fact, a considerable amount of literature exists on the inhibitory or toxic effects resulting from lactate and ammonia accumulation in mammalian cell cultures 31 . Specifically, an inhibitory effect on cell growth and antibody production has been reported for these metabolic by-products 11,19,29 .…”

“…Cell growth and antibody generation are conditioned by the availability of amino acids in the medium 31 . For example, the presence of glutamine is a necessary condition for MAb production.…”

Use of Immobilized Candida Cells on Xylitol Production from Sugarcane Bagasse

“…Ferm enta tions in repeated batch mode are appropriate for this bioprocess because they prom ote a good adaptation of the cells to the hydrolysate. In this sense, the immobilized cell system has been re commended not only for maximizing the fer m ented broth fraction discharged at the end of each batch but also for facilitating the reutilization of the biocatalysts, for minimizing times and sepa ration costs, and for making the cells more stable (G am arra et al, 1986;Dervakos and Webb, 1991;Lother and Oetterer, 1995;Dominguez, 1998).…”