On the Mechanism of the Hydrolysis of Glycerides by Pancreatic Lipase.

Borgström, Bengt; Grahn, Margareta; Fex, H.; Högberg, Björn; Linderot, T.; Rosenberg, Th.

doi:10.3891/acta.chem.scand.07-0557

Cited by 64 publications

(10 citation statements)

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“…When a mixture of radioactive palmitic acid and triglycerides is treated by lipase, the free acid mostly exchanges with outer chains (64). Diolein arising from triolein is also the 1,a-isomer (64).…”

Section: Demonstrationmentioning

confidence: 99%

Pancreatic Lipase

Desnuelle¹

1961

Advances in Enzymology - And Related Areas of Molecular Biology

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Section: Demonstrationmentioning

confidence: 99%

Pancreatic Lipase

Desnuelle¹

1961

Advances in Enzymology - And Related Areas of Molecular Biology

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“…This observation indicated that the hydrolysis of triglycerides in the intestinal tract of the rat involved a series of step-wise reactions from triglycerides to 1,2-diglycerides to 2-monoglycerides, with fatty acids released at each step. Recently Borgstrom (12) demonstrated that these same reactions occur during the in tro hydrolysis of triglycerides by rat pancreatic lipase.…”

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confidence: 99%

Digestion of Neutral Fats by Human Subjects 1

Harris¹,

Chamberlain²,

Benedict³

1955

J. Clin. Invest.

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Several theories have been advanced to describe the digestion of fats in animals and in humans. Of these, two have been popular. The hydrolytic theory (1) suggests that triglycerides are broken down completely to fatty acids and glycerol which are then absorbed into the intestinal wall. The particulate theory (2) suggests that triglycerides are partially hydrolyzed in the intestinal lumen to monoglycerides, diglycerides, and fatty acids. The partial glycerides, fatty acids, and bile salts cause a fine emulsification of the remaining triglyceride. This emulsion, containing triglycerides, then enters the intestinal wall.Much indirect evidence exists for the formation of partial glycerides during fat digestion. Reiser, Bryson, Carr, and Kuiken (3) analyzed the lipids recovered from the thoracic ducts of rats fed tagged triglyceride and concluded that the major portion of ingested fat is hydrolyzed to monoglyceride prior to absorption. Frazer and Sammons (4) noted that lipids isolated from the lumen of the intestinal tract of rats had a high hydroxyl value and concluded that monoglyceride was present. Later Frazer (5) reported, without presenting experimental details, that he had found monoglycerides in the intestinal tracts of human subjects. The formation and presence of monoglycerides in the intestinal tract during the digestion of fat has been reported in the dog (6), rat (3, 4, 7-9), cat (10), monkey (8), and man (5, 11).Theoretically the formation of two isomeric monoglycerides is possible when a triglyceride is digested to monoglyceride. It was not until the recent work of Mattson, Benedict, Martin, and Beck (7) that 2-monoglycerides were shown to 1

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“…They have been shown to stimulate the action of pancreatic lipase (2) and have an important emulsifying action on water-insoluble dietary triglycerides that is probably related to their ability to form micelles with fatty acids and monoglycerides (3). In addition to their role in triglyceride absorption, bile salts also appear to be essential in the absorption and esterification of vitamin A (4) and cholesterol (5).…”

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confidence: 99%

Effects of Bile Salts on Glucose Metabolism by Slices of Hamster Small Intestine*

Holt¹,

Haessler²,

Isselbacher³

1963

J. Clin. Invest.

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Bile salts are known to be essential for the effective absorption of fat from the intestinal tract (1). They have been shown to stimulate the action of pancreatic lipase (2) and have an important emulsifying action on water-insoluble dietary triglycerides that is probably related to their ability to form micelles with fatty acids and monoglycerides (3). In addition to their role in triglyceride absorption, bile salts also appear to be essential in the absorption and esterification of vitamin A (4) and cholesterol (5).In previous studies from this laboratory (6), it was shown that conjugated bile salts enhanced the esterification of palmitate-C14 to triglycerides and appeared to stimulate the incorporation of glucose-C14 into glyceride-glycerol. These observations suggested that in the intestinal mucosa, bile salts influence the metabolism not only of lipid, but also of carbohydrate. The present experiments were designed to investigate further the actions of conjugated and unconjugated bile salts on glucose metabolism. These studies have been carried out with slices of hamster small intestine. In addition, because of some disagreement in the literature concerning the utilization of glycerol in the intestine (7,8), the metabolism of glucose and glycerol by hamster intestinal mucosa has been compared. EXPERIMENTAL PROCEDUREMaterials. Male golden hamsters 1 weighing 100 to 120 g were used in all experiments. Glucose-1-Cl4 2 and glucose-6-Cl42 were checked for purity on Dowex columns. Batches of glucose-C14 with more than 0.5% im-

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On the Mechanism of the Hydrolysis of Glycerides by Pancreatic Lipase.

Cited by 64 publications

References 0 publications

Pancreatic Lipase

Pancreatic Lipase

Digestion of Neutral Fats by Human Subjects 1

Effects of Bile Salts on Glucose Metabolism by Slices of Hamster Small Intestine*

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