On the mechanism of Mallory's phosphotungstic acid‐haematoxylin stain

Puchtler, Holde; Waldrop, FayeSweat; Meloan, SusanN.

doi:10.1111/j.1365-2818.1980.tb04109.x

Cited by 21 publications

(5 citation statements)

References 18 publications

(11 reference statements)

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“…This conclusion was drawn from the differences resulting from the used detection methods. The antibodies bind to actin regardless its quarterly structure [59], which on the other hand is crucial for the phosphotungstic acid hematoxylin staining [60]. Curcumin has been shown to suppress the vascular smooth muscle cells migration and proliferation [61] and to differentially interfere with proteins translation [62], which may correlate with correction of the fibrilar proteins content in the aortic media.…”

Section: Discussionmentioning

confidence: 99%

Spice up the hypertension diet - curcumin and piperine prevent remodeling of aorta in experimental L-NAME induced hypertension

et al. 2011

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BackgroundIncrease of blood pressure is accompanied by functional and morphological changes in the vascular wall. The presented study explored the effects of curcuma and black pepper compounds on increased blood pressure and remodeling of aorta in the rat model of experimental NO-deficient hypertension.MethodsWistar rats were administered for 6 weeks clear water or L-NAME (40 mg/kg/day) dissolved in water, piperine (20 mg/kg/day), curcumin (100 mg/kg/day) or their combination in corn oil by oral gavage. The systolic blood pressure was measured weekly. Histological slices of thoracic aorta were stained with hematoxylin and eosin, Mallory's phosphotungstic acid hematoxylin (PTAH), orcein, picrosirius red and van Gieson staining and with antibodies against smooth muscle cells actin. Microscopic pictures were digitally processed and morphometrically evaluated.ResultsThe increase of blood pressure caused by L-NAME was partially prevented by piperine and curcumin, but the effect of their combination was less significant. Animals with hypertension had increased wall thickness and cross-sectional area of the aorta, accompanied by relative increase of PTAH positive myofibrils and decrease of elastin, collagen and actin content. Piperine was able to decrease the content of myofibrils and slightly increase actin, while curcumin also prevented elastin decrease. The combination of spices had similar effects on aortic morphology as curcumin itself.ConclusionsAdministration of piperine or curcumin, less their combination, is able to partially prevent the increase of blood pressure caused by chronic L-NAME administration. The spices modify the remodeling of the wall of the aorta induced by hypertension. Our results show that independent administration of curcumin is more effective in preventing negative changes in blood vessel morphology accompanying hypertensive disease.

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Section: Discussionmentioning

confidence: 99%

Spice up the hypertension diet - curcumin and piperine prevent remodeling of aorta in experimental L-NAME induced hypertension

et al. 2011

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“…In this study, Phosphomolybdic Acid (PMA), which targets collagen residues 23 , 24 , was utilized in combination with 1- and 6- micron spatial resolution microCT to assess the postnatal development of mouse lumbar intervertebral discs up to a year of age. Moreover, we specifically report quantitative three-dimensional metrics: the notochord surface area to volume ratio (S.a/V), which defines the structure of the notochord, and the notochord volume.…”

Section: Introductionmentioning

confidence: 99%

Postnatal Development of the Murine Notochord Remnants Quantified by High-resolution Contrast-enhanced MicroCT

Bhalla

Lin

Tang

2017

Sci Rep

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The notochord gives rise to spinal segments during development, and it becomes embedded within the nucleus pulposus of the intervertebral disc (IVD) during maturation. The disruption of the notochord band has been observed with IVD degeneration. Since the mechanical competence of the IVD relies on its structural constituents, defining the structure of the notochord during aging is critical for investigations relating to IVD function and homeostasis. The assessment and imaging of the notochord has classically relied on histological techniques, which introduces sectioning artifacts during preparation and spatial biases. Magnetic resonance imaging (MRI) does not offer sufficient resolution to discriminate the notochord from the surrounding the nucleus pulposus, especially in murine models. Current X-ray based computed tomography systems provide imaging resolutions down to the single- and sub- micron scales, and when coupled with contrast-enhancing agents, enable the high-resolution three-dimensional imaging of relatively small features. Utilizing phosphomolybdic acid to preferentially bind to collagen cationic domains, we describe the structure of the notochord remnants with aging in the lumbar IVDs of BALB/c mice. These results provide a highly quantitative and sensitive approach to monitoring the IVD during postnatal development.

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“…In contrast, apart from the aforementioned microorganisms within intestinal lumina, both dipterans showed magenta, coarse to finely granular cytoplasm in ovaries, mosquitoes in hindgut epithelium, and fruit flies in cortical ganglion cells (Figs. [45][46][47]. Similar staining patterns were displayed in salivary gland, cardia/proventriculus, and midgut epithelium of mosquitoes and fruit flies.…”

Section: Stain For Microorganismsmentioning

confidence: 84%

“…However, PTAH was best at highlighting musculature, as previously reported, 6 given that striated, smooth, and heart musculature were ascertainable. As this dye also binds to Z-bands, 47 cross-striation of muscle fibers was most obvious with PTAH in equine and dipterans, which renders it particularly interesting for the evaluation of myopathies in all species investigated.…”

Section: Discussionmentioning

confidence: 99%

Histochemical staining techniques in Culex pipiens and Drosophila melanogaster (Diptera) with a comparison to mammals

Gregor¹,

Becker²,

Hellhammer³

et al. 2022

Vet Pathol

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Insects play an important role in ecosystems. Changes in their abundance and biodiversity are of paramount interest, as there has not only been an alarming decline of insects important for ecosystem health throughout the past decades, but also an increase in insects detrimental for biomes. Furthermore, insects pose a threat to modern society as arbovirus-transmitting vectors. Therefore, detailed knowledge of insect staining characteristics could be beneficial as a basis for further studies, whether in the context of species conservation or control of insect pests. Thus, this study compared 14 histochemical stains for their usefulness in insects regarding nervous tissue, connective tissue components, mucins and polysaccharides, mineralization, and microorganisms. The study used formalin-fixed paraffin-embedded tissue sections of mammals ( Equus caballus) and 2 dipterans ( Culex pipiens biotype molestus, Drosophila melanogaster). Several histochemical stains were suitable for tissue assessment in insects and mammals, in particular for nervous tissue (Bielschowsky silver stain, luxol fast blue–cresyl violet) and polysaccharides (alcian blue, periodic acid–Schiff with and without diastase treatment, toluidine blue). Other stains proved useful for visualization of insect-specific organ characteristics such as Gomori’s reticulin stain for tracheoles in both dipteran species, Heidenhain’s azan for midgut-associated connective tissue, and von Kossa for mineral deposition in Malpighian tubules of C. pipiens biotype molestus. In summary, this study provides comparable insights into histochemical procedures in mammals and insects and their usefulness for histological assessment of C. pipiens biotype molestus and D. melanogaster.

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On the mechanism of Mallory's phosphotungstic acid‐haematoxylin stain

Cited by 21 publications

References 18 publications

Spice up the hypertension diet - curcumin and piperine prevent remodeling of aorta in experimental L-NAME induced hypertension

Spice up the hypertension diet - curcumin and piperine prevent remodeling of aorta in experimental L-NAME induced hypertension

Postnatal Development of the Murine Notochord Remnants Quantified by High-resolution Contrast-enhanced MicroCT

Histochemical staining techniques in Culex pipiens and Drosophila melanogaster (Diptera) with a comparison to mammals

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