On the mechanism of inhibition of the sulfate transfer across the human erythrocyte membrane

Schnell, K. F.

doi:10.1016/0005-2736(72)90333-1

Cited by 78 publications

(43 citation statements)

References 8 publications

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“…Thus, as has been shown for red cell membranes (Gerlach, Deuticke & Duhm, 1964;Schnell, 1972;Passow & Wood, 1974), phlorizin might inhibit anion transport in the proximal tubule, and so secondarily reduce sodium (and water) flux apart from its action on glucose transport. Alternatively, the small amount of glucose secreted into the tubule might reduce net osmotic efflux of fluid, both directly and via any effect on sodium cotransport; or any effects of phlorizin on the availability of glucose as on intracellular metabolic substrate might interfere with fluid reabsorption.…”

Section: Effects Of Phlorizinmentioning

confidence: 73%

Effects of glucose on water and sodium reabsorption in the proximal convoluted tubule of rat kidney.

Bishop¹,

Green²,

Thomas³

1978

The Journal of Physiology

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SUMMARY1. The effects of glucose on sodium and water reabsorption by rat renal proximal tubules was investigated by in situ microperfusion of segments of proximal tubules with solutions containing glucose or no glucose, with and without phlorizin.2. Absence of glucose did not significantly alter net water flux. Sodium flux was reduced by about 10 % but this was not statistically significant.3. In the absence of glucose in the perfusion fluid net secretion of glucose occurred. 4. Phlorizin reduced either net reabsorption or net secretion of glucose; and net water flux.5. The data suggest that in later parts of the proximal convoluted tubule some sodium may be co-transported with glucose, but that this represents only a small fraction of the total sodium reabsorption.6. It is suggested that the glucose carrier is reversible and in appropriate circumstances could cause glucose secretion.7. Althoughphlorizin alters net water flux the underlying mechanisms are not clear. 8. The calculated osmolality of the reabsorbate was significantly greater than the perfusate osmolality and greater than plasma osmolality although this was not quite significant statistically.

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Section: Effects Of Phlorizinmentioning

confidence: 73%

Effects of glucose on water and sodium reabsorption in the proximal convoluted tubule of rat kidney.

Bishop¹,

Green²,

Thomas³

1978

The Journal of Physiology

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“…I) suggests that Na+-glucose cotransport operates on the luminal side of the pleural mesothelium and contributes to the solute-coupled liquid absorption. Since 10-3 M phloridzin has been found to partially inhibit anion exchange (Schnell, 1972;Chow & , 1982;Turner & George, 1988), one could argue that the decrease in Jnet found in the hydrothoraces with phloridzin might be due to inhibition of the Cl--HCO3-exchanger, rather than of Na+-glucose cotransport. This, however, is not the case because the decrease in Jnet produced by 10-4 M phloridzin (that does not inhibit the exchanger) is similar to that produced by 10-3 M phloridzin.…”

Section: Resultsmentioning

confidence: 99%

Effect on phloridzin on net rate of liquid absorption from the pleural space of rabbits

Zocchi¹,

Agostoni²,

Raffaini³

1996

Experimental Physiology

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SUMMARYPrevious indirect findings have suggested the occurrence of solute-coupled liquid absorption from the pleural space, consistent with Na+-Kt-ATPase on the interstitial side plus a Nat-Ht and Cl--HCO3-double exchange on the luminal side of the pleural mesothelium. To assess whether Nat-glucose cotransport also operates on the luminal side, the relationship between net rate of liquid absorption from the right pleural space (Vnet) and volume of liquid injected into this space (0.5, 1 or 2 ml) was determined in anaesthetized rabbits during hydrothoraces with phloridzin (10-3 M) or with phloridzin plus 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid (SITS; 1.5 x 10-4 M). The relationship obtained during hydrothoraces with phloridzin was displaced downwards by 0.09 ml h-' relative to that in control hydrothoraces (P < 0.01). The decrease in Jnet was similar in hydrothoraces of various sizes. The relationship obtained in hydrothoraces with phloridzin plus SITS was displaced downwards by 0. 16 ml h-' relative to that in control hydrothoraces (P < 0.01), i.e. the decrease in Jnet was similar to the sum (0.17 ml h-') of the decreases in Jnet produced individually by phloridzin and by SITS (0.08 ml h-'). The decrease in Jnet was similar in hydrothoraces of differing size. The above findings are consistent with the occurrence of Nat-glucose cotransport on the luminal side of the pleural mesothelium, operating simultaneously with the double exchange also under physiological conditions.

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“…Specific trapped volumes in all three preparations (see legend to Table 2 Rothstein, 1972). Persantine, one of the most effective non-competitive inhibitors (Schnell, 1972), did not inhibit the flux at a concentration of 0.1 mm.…”

Section: Structural Characterization Ofreconstituted Vesiclesmentioning

confidence: 95%

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

Wolosin¹

1980

Biochemical Journal

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A short procedure for the isolation of band-3 protein, the protein responsible for anion exchange in erythrocytes, in a reasonable degree of purity was developed. Using this protein preparation and a novel procedure for membrane-protein reconstitution, vesicles displaying the basic features of the anion-exchange system of the erythrocyte were obtained. The reconstitution procedure is based on slow direct removal of Triton X-100 from aqueous lipid/detergent solutions. According to the composition of the reconstitution medium, either small single-walled or large multi-walled vesicles are obtained. The procedure conserves protein properties well, as is revealed by the similarity of the rates of SQ42-exchange in erythrocytes and reconstituted vesicles when corrected for the relevant volumes. A number of functional features of the exchange system were studied and compared with those of the native membrane.

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On the mechanism of inhibition of the sulfate transfer across the human erythrocyte membrane

Cited by 78 publications

References 8 publications

Effects of glucose on water and sodium reabsorption in the proximal convoluted tubule of rat kidney.

Effects of glucose on water and sodium reabsorption in the proximal convoluted tubule of rat kidney.

Effect on phloridzin on net rate of liquid absorption from the pleural space of rabbits

A procedure for membrane-protein reconstitution and the functional reconstitution of the anion transport system of the human-erythrocyte membrane

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