On the Mechanism of Action of the Antibiotic
            <i>O</i>
            -Carbamyl-
            <scp>d</scp>
            -Serine in
            <i>Streptococcus faecalis</i>

Lynch, Judith L.; Neuhaus, Francis C.

doi:10.1128/jb.91.1.449-460.1966

Cited by 48 publications

(15 citation statements)

References 32 publications

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“…UDP-MurNAc:L-Ala ligase. D-Cycloserine (1 mM) was added to the assay mixture to inhibit racemization of the labeled L-alanine substrate and its subsequent dimerization to D-Ala:D-Ala (cf 24,29,38). Enzymes prepared using the 20 mM KPO, buffer, pH 7.8, gave only very low activities.…”

Section: Resultsmentioning

confidence: 99%

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Cell Wall Polymers of Bacillus sphaericus : Activities of Enzymes Involved in Peptidoglycan Precursor Synthesis During Sporulation

Linnett

Tipper

1974

J Bacteriol

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In synchronously sporulating cells of Bacillus sphaericus 9602, the specific activities of those enzymes specifically required for the synthesis of the UDP-Nacetyl-muramyl-pentapeptide precursor of vegetative cell wall peptidoglycan decay by 50% after the end of exponential cell division, probably as a consequence of dilution by newly synthesized protein. The meso-diaminopimelate ligase is the only new activity whose synthesis is required for synthesis of the nucleotide-pentapeptide precursor of spore cortex peptidoglycan. The addition of D-Ala-D-Ala to the nucleotide tripeptide is catalyzed by an enzyme present in both vegetative and sporulating cells, which apparently does not discriminate between lysineand diaminopimelate-containing acceptors. The activities of the L-Ala and D-Ala-D-Ala ligases and of the D-Ala-D-Ala synthetase increases in parallel with the appearance of the diaminopimelate ligase, indicating coordinate derepression and suggesting operon-like organization of the appropriate structural genes.

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Section: Resultsmentioning

confidence: 99%

“…The structure of the cortical peptidoglycan of B. sphaericus spores (D. J. Tipper, Bacteriol. Proc., p. 24,1969) is identical to that of Bacillus subtilis (42, 43, cf 40). All contain unique component not yet found in any cell wall peptidoglycan.…”

mentioning

confidence: 82%

Cell Wall Polymers of Bacillus sphaericus : Activities of Enzymes Involved in Peptidoglycan Precursor Synthesis During Sporulation

Linnett

Tipper

1974

J Bacteriol

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“…Solutions of D-[V4C]Cycloserine were standardized by the method of Jones (12). The concentration of O-carbamyl-D-[14C]serine was routinely determined by ion-exchange chromatography on an amino acid analyzer (19). Corp. All other materials were reagent grade.…”

Section: Mechanism Of D-cycloserine Actionmentioning

confidence: 99%

Mechanism of d -Cycloserine Action: Transport Systems for d -Alanine, d -Cycloserine, l -Alanine, and Glycine

1970

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The accumulation of D-alanine, L-alanine, glycine, and D-cycloserine in Escherichia coli was found to be mediated by at least two transport systems. The systems for D-alanine and glycine are related, and are separate from that involved in the accumulation of L-alanine. D-Cycloserine appears to be primarily transported by the D-alanine-glycine system. The accumulation of D-alanine, glycine, and D-cycloserine was characterized by two line segments in the Lineweaver-Burk analysis, whereas the accumulation of L-alanine was characterized by a single line segment. D-CyclOserine was an effective inhibitor of glycine and D-alanine accumulation, and L-cyclOserine was an effective inhibitor of L-alanine transport. The systems were further differentiated by effects of azide, enhancement under various growth conditions, and additional inhibitor studies. Since the primary access of D-cycloserine in E. coli is via the D-alanine-glycine system, glycine might be expected to be a better antagonist of D-cycloserine inhibition than L-alanine. Glycine and D-alanine at 10-M antagonized the effect of D-cycloserine in E. coli, whereas this concentration of L-alanine had no effect.

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“…The suspension was centrifuged at 100,000 x g for I hr. Samples of the supernatant fraction were assayed for alanine racemase (4) and D-alanine: D-alanine ligase (ADP) (9). has been lost.…”

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confidence: 99%

Mechanism of d -Cycloserine Action: Transport Mutants for d -Alanine, d -Cycloserine, and Glycine

1971

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The accumulation of D-alanine and the accumulation of glycine in Escherichia coli are related and appear to be separate from the transport of L-alanine. The analysis of four D-cycloserine-resistant mutants provides additional-support for this conclusion. The first-step mutant from E. coli K-12 that is resistant to D-cycloserine was characterized by the loss of the high-affinity line segment of the D-alanine-glycine transport system in the Lineweaver-Burk plot. This mutation, which is linked to the met, locus, also resulted in the loss of the ability to transport D-cycloserine. The second-step mutation that is located 0.5 min from the first-step mutation resulted in the loss of the low-affinity line segment for the D-alanine-glycine transport system. The transport of L-alanine was decreased only 20 to 30% in each of these mutants. A multistep mutant from E. coli W that is 80-fold resistant to D-cycloserine lost >90% of the transport activity for D-alanine and glycine, whereas 75% of the transport activity for L-alanine was retained. E. coli W could utilize either Dor L-alanine as a carbon source, whereas the multistep mutant could only utilize Lalanine. Thus, a functioning transport system for D-alanine and glycine is required for both D-cycloserine action and growth on D-alanine.

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On the Mechanism of Action of the Antibiotic O -Carbamyl- d -Serine in Streptococcus faecalis

Cited by 48 publications

References 32 publications

Cell Wall Polymers of Bacillus sphaericus : Activities of Enzymes Involved in Peptidoglycan Precursor Synthesis During Sporulation

Cell Wall Polymers of Bacillus sphaericus : Activities of Enzymes Involved in Peptidoglycan Precursor Synthesis During Sporulation

Mechanism of d -Cycloserine Action: Transport Systems for d -Alanine, d -Cycloserine, l -Alanine, and Glycine

Mechanism of d -Cycloserine Action: Transport Mutants for d -Alanine, d -Cycloserine, and Glycine

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