On the measurement of human osteosarcoma cell elastic modulus using shear assay experiments

Cao, Yifang; Bly, Randy; Moore, W. A.; Gao, Zhan; Cuitiño, Alberto M.; Soboyejo, W. O.

doi:10.1007/s10856-006-0667-8

Cited by 10 publications

(3 citation statements)

References 16 publications

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“…The shear assay experiment was used in this study to exert mechanical forces in the form of fluid pressure to biological cells (MCF-10A and MDA-MB-231), and hence the mechanical response of these cells to an applied force was subsequently observed. 40 This, however, translates to the further determination of the cell viscoelastic properties of the non-tumorigenic and tumorigenic breast cells using other mechanical techniques such as strain mapping and the use of viscoelastic models. An average of 20 single cells per cell line was sampled for this study.…”

Section: Shear Assay Experimentsmentioning

confidence: 99%

Investigation of creep properties and the cytoskeletal structures of non‐tumorigenic breast cells and triple‐negative breast cancer cells

Onwudiwe

Obayemi

et al. 2021

J Biomedical Materials Res

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This article presents the correlation of creep and viscoelastic properties to the cytoskeletal structure of both tumorigenic and non‐tumorigenic cells. Unique shear assay and strain mapping techniques were used to study the creep and viscoelastic properties of single non‐tumorigenic and tumorigenic cells. At least 20 individual cells, three locations per cell, were studied. From the results, lower densities in the volume of actin, and keratin 18 structures were observed with the progression of cancer and were correlated to the increased creep rates and reduced mechanical properties (Young's moduli and viscosities) of tumorigenic (MDA‐MB‐231) cells. The study reveals significant differences between the creep and viscoelastic properties of non‐tumorigenic breast cells versus tumorigenic cells. The variations in the creep strain rates are shown to be well characterized by lognormal distributions, while the statistical variations in the viscoelastic properties are well‐described by normal distributions. The implications of the results are discussed for the study of discrete cell behaviors, strain and viscoelastic responses of the cell, and the role of cell cytoskeleton in the onset and progression of cancers.

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Section: Shear Assay Experimentsmentioning

confidence: 99%

Investigation of creep properties and the cytoskeletal structures of non‐tumorigenic breast cells and triple‐negative breast cancer cells

Onwudiwe

Obayemi

et al. 2021

J Biomedical Materials Res

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“…Linear elastic [Charras and Horton 2002] § [Cao et al 2007;Ferko et al 2007 Nonlinear elastic [Dao et al 2003;Mills et al 2004] [Suresh et al 2005] Modified Maxwell [Mills et al 2004] viscoelastic Table 1. Computational models for whole cell deformation in common experimental techniques in cell mechanics.…”

Section: Shear Flowmentioning

confidence: 99%

Continuum-based computational models for cell and nuclear mechanics

Vaziri

Gopinath

Deshpande

2007

JOMMS

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Deciphering the relationship between cellular processes and the structure of living cells is a key step toward understanding and predicting cell functions with direct implications for understanding human health and disease. The active nature of these cellular processes, which span several decades of spatial and temporal scales, pose significant challenges to unraveling this complex structure-function paradigm.Complementing novel experimental techniques with robust computational approaches capable of modeling mechanical response at varying scales provides new avenues to resolving this paradigm. We provide an overview of continuum-based computational approaches used in studying and interpreting responses of individual cells and nuclei, we outline techniques used for measuring the mechanical characteristics of living cells, and we discuss some of the key insights provided by these approaches.

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“…The use of this in biological instances is often limited due to the need for a high contrast pattern, however analysis has been achieved and used in large scale cell migration studies [10]. Images of cell deformation at higher temporal resolution and magnifications with sufficient contrast for DIC are far more challenging to achieve [11,12].…”

Section: Introductionmentioning

confidence: 99%

The image-based ultrasonic cell shaking test

Ballard,

Marek,

Pierron

2023

PLoS ONE

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Mechanical signals play a vital role in cell biology and is a vast area of research. Thus, there is motivation to understand cell deformation and mechanobiological responses. However, the ability to controllably deform cells in the ultrasonic regime and test their response is a noted challenge throughout the literature. Quantifying and eliciting an appropriate stimulus has proven to be difficult, resulting in methods that are either too aggressive or oversimplified. Furthermore, the ability to gain a real-time insight into cell deformation and link this with the biological response is yet to be achieved. One application of this understanding is in ultrasonic surgical cutting, which is a promising alternative to traditional methods, but with little understanding of its effect on cells. Here we present the image based ultrasonic cell shaking test, a novel method that enables controllable loading of cells and quantification of their response to ultrasonic vibrations. Practically, this involves seeding cells on a substrate that resonates at ultrasonic frequencies and transfers the deformation to the cells. This is then incorporated into microscopic imaging techniques to obtain high-speed images of ultrasonic cell deformation that can be analysed using digital image correlation techniques. Cells can then be extracted after excitation to undergo analysis to understand the biological response to the deformation. This method could aid in understanding the effects of ultrasonic stimulation on cells and how activated mechanobiological pathways result in physical and biochemical responses.

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On the measurement of human osteosarcoma cell elastic modulus using shear assay experiments

Cited by 10 publications

References 16 publications

Investigation of creep properties and the cytoskeletal structures of non‐tumorigenic breast cells and triple‐negative breast cancer cells

Investigation of creep properties and the cytoskeletal structures of non‐tumorigenic breast cells and triple‐negative breast cancer cells

Continuum-based computational models for cell and nuclear mechanics

The image-based ultrasonic cell shaking test

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