On the measurement of consistent long-term retention factor values in micellar liquid chromatography

The capability of biopartitioning micellar chromatography (BMC), using pure Brij35 solution and mixed micellar system of Brij35-SDS (85:15) as mobile phase, to describe and estimate bioactivities of angiotensin converting enzyme inhibitors at different pH has been studied. Quantitative retention-activity relationships (QRAR) in BMC were investigated for these compounds. The obtained BMC(Brij35-SDS)-QRAR models were compared with the traditional BMC(Brij35)-QRAR, and better statistically models were obtained using Brij35-SDS retention data. The superiority of BMC(Brij35-SDS)-QRAR is due to the fact that the mixed micellar mobile phase can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged.

Section: Methodsmentioning

confidence: 99%

Mixed micellar liquid chromatography methods: modelling quantitative retention–activity relationships of angiotensin converting enzyme inhibitors

Cui

Xiong

et al. 2008

“…The retention data (k) in BMC were calculated according to the IUPAC approach (Bermúdez-Saldaña et al, 2007), based on the extra-column time correction, which for any chemical can be estimated as: Reagents and standards. Norfloxacin was kindly donated by the Institute of Antibiotics (Sichuan, China).…”

Section: Methodsmentioning

confidence: 99%

Quantitative retention–activity relationship models for quinolones using biopartitioning micellar chromatography

Chen

Wang

et al. 2007

A simple and reproducible quantitative retention-activity relationship (QRAR) model utilizing biopartitioning micellar chromatography was developed for the biological parameter estimation of drugs. The correlation between retention factors of quinolones obtained in physiological conditions (pH, ionic strength) and biological activities was investigated using different second-order polynomial models. The predictive and interpretative ability of the chromatographic models was evaluated in terms of cross-validated data (RMSEC, RMSECV and RMSECVi). The aim was to obtain adequate QRAR models of half-life, clearance, volume of distribution, plasma protein combination rate, area under concentration-time curve and toxicity (LD50) of quinolones, and to elucidate the advantages and limitations of using a single parameter as independent variable for describing and estimating the activities.

“…The retention data in BMC were calculated according to the IUPAC approach (Bermúdez-Saldaña et al, 2007), based on the extra-column time correction, for any chemical can be estimated as:…”

Section: Experimental Instrumental and Measurementmentioning

confidence: 99%

Development of predictive quantitative retention–activity relationship models of alkaloids by mixed micellar liquid chromatography

Chen

et al. 2009

The mixed micellar liquid chromatography is a mode that uses mixed micellar system of Brij35/SDS (85 : 15) as a mobile phase under adequate experimental conditions, can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged. In this article, the applications of biopartitioning micellar chromatography, using mixed micellar system to describe and estimate bioactivities of alkaloids, has been focused. The BMC(Brij35/SDS)-QRAR models of half-life time, volume of distribution, plasma clearance and area under concentration-time curve were obtained using Brij35-SDS retention data. The aim is to take a look at the capability of the mixed micellar liquid chromatography model to describe and/or estimate the bioactivity of alkaloids.