On the localized coupling of respiration and phosphorylation in mitochondria

Yaguzhinsky, L. S.; Yurkov, V. I.; Krasinskaya, Inna P.

doi:10.1016/j.bbabio.2006.04.001

Cited by 20 publications

(14 citation statements)

References 23 publications

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“…Likewise, specific respiratory supercomplexes were detected by similar approaches in mitochondria of various eukaryotes (15,26,27,45,47,61,62,64,71,94,97,98), including the filamentous fungus Podospora anserina (45), as well as in bacteria (79). Recently, the first single particle structures of respiratory supercomplexes were determined (24,39,66), and several other important studies by the bioenergetics/ mitochondrial community have supported the respirasome model (1,6,10,18,21,49,56,62,85,86,88,96). However, the functional significance of respiratory supercomplexes, including their enzymatic advantages, is still poorly documented.…”

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confidence: 88%

Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria

et al. 2007

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The existence of specific respiratory supercomplexes in mitochondria of most organisms has gained much momentum. However, its functional significance is still poorly understood. The availability of many deletion mutants in complex I (NADH:ubiquinone oxidoreductase) of Neurospora crassa, distinctly affected in the assembly process, offers unique opportunities to analyze the biogenesis of respiratory supercomplexes. Herein, we describe the role of complex I in assembly of respiratory complexes and supercomplexes as suggested by blue and colorless native polyacrylamide gel electrophoresis and mass spectrometry analyses of mildly solubilized mitochondria from the wild type and eight deletion mutants.As an important refinement of the fungal respirasome model, we found that the standard respiratory chain of N. crassa comprises putative complex I dimers in addition to I-III-IV and III-IV supercomplexes. Three Neurospora mutants able to assemble a complete complex I, lacking only the disrupted subunit, have respiratory supercomplexes, in particular I-III-IV supercomplexes and complex I dimers, like the wild-type strain. Furthermore, we were able to detect the I-III-IV supercomplexes in the nuo51 mutant with no overall enzymatic activity, representing the first example of inactive respirasomes. In addition, III-IV supercomplexes were also present in strains lacking an assembled complex I, namely, in four membrane arm subunit mutants as well as in the peripheral arm nuo30.4 mutant. In membrane arm mutants, high-molecular-mass species of the 30.4-kDa peripheral arm subunit comigrating with III-IV supercomplexes and/or the prohibitin complex were detected. The data presented herein suggest that the biogenesis of complex I is linked with its assembly into supercomplexes.

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confidence: 88%

Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria

et al. 2007

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“…In that work it was found that activation of Н + pumps decreases nega tive ζ potential and a catalyst (HEPES) is able to elim inate this effect. In a previous work [3] it was shown that catalyst (HEPES 20 mM) significantly increases the maximum value of the transmembrane flux of H + ions.…”

Section: Introductionmentioning

confidence: 92%

“…In our previous works [3,4], the formation of metastable fraction of hydrogen ions possessing an excess of free energy was demonstrated on the mem brane surface of mitoplasts [5] and mitochondria [6]. Membrane bound protons stay in a non equilibrium state and participate in the reaction of the ATP synthe sis [3,4].…”

Section: Introductionmentioning

confidence: 99%

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Specificity of interactions of the surface-active protonophore 2,4,6-trichloro-3-pentadecylphenol with artificial and mitochondrial membranes

Dzhumashev

Byvshev

Eremeev

et al. 2015

Biochem. Moscow Suppl. Ser. A

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Membranotropic properties of the surface active reagent 2,4,6 trichloro 3 pentadecylphenol (TCP C15) have been studied. In preliminary investigations it was found that TCP C15 acts as an uncoupler of oxidative phosphorylation, selectively interacting with a fraction of non equilibrium membrane bound protons that forms during the work of proton pumps. In this work we obtained the following results: (1) addi tional validation of the conclusions drawn from the previous experiments was acquired on modified prepara tions of liver mitochondria, in which the fraction of non equilibrium membrane bound protons did not form;(2) it was shown that binding of TCP C15 to the liposome surface increases its negative ζ potential; this proves that TCP C15 binds to the surface of membranes in the negatively charged state; (3) on the BLM model it was found that under constant electric field (90 mV) TCP C15 not only induces the transmembrane transfer of charge, but also induces a specific hydrogen ion affinity to the surface of lipid bilayer; (4) strong evidence of the ionophore activity of TCP C15 was obtained on the same model; this allowed us to get a full scheme of uncoupling mechanism of TCP C15 on mitochondria and to explain the action of a catalyst (HEPES, 20 mM), which blocks this mechanism.Keywords: mitochondria, proton pump, non equillibrium membrane bound protons, interphase binding of proton

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“…It was shown that the solvation potential energy could be used for synthesis of ATP and that the catalyst of [12,16]. This result suggested that the fraction of membrane bound protons was involved in the synthe sis of ATP.…”

Section: Fraction Of "Energized" Protons On the Surface Of Membranes mentioning

confidence: 97%

On local coupling of electron transport and ATP-synthesis system in mitochondria. Theory and experiment

Eremeev

Yaguzhinsky

2015

Biochemistry Moscow

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A brief description of the principal directions for searching and investigating the model of local coupling between respiration and phosphorylation proposed by R. Williams is given in this paper. We found conditions where it was possible to reveal typical functional special features of the mitochondrial phosphorylating system. According to the theory, such special features should be observed experimentally if the mitochondrial phosphorylating system operated in the state of a supercomplex. It was proved that the phosphorylating system is able to operate in two states: P. Mitchell state and R. Williams state. It was demonstrated that in the ATP synthesis reaction, ATP-synthase (F1F0) was able to use thermodynamic potential of Bronsted acids as a source of energy. It was shown using a double-inhibitor titration technique that when the phosphorylating system operated in the supercomplex state, the electron transfer system and ATP-synthesis system were docked rigidly. A model system of chemical synthesis of membrane-bound proton fraction (Bronsted acids), carrying a free energy excess, was developed on the model of bilayer lipid membrane. Catalysts selectively accelerating proton detachment of this fraction were also found. The formation of a Bronsted acids fraction carrying free energy excess was recorded during the operation of proton pumps on mitochondrial and mitoplastic membranes. In the experimental part of the work, a brief description is given of studies on new uncouplers that transfer the phosphorylation system from the local coupling state to the state of transmembrane proton transfer. Thus, they accelerated the respiration of mitochondria and decreased the ADP/O parameter.

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On the localized coupling of respiration and phosphorylation in mitochondria

Cited by 20 publications

References 23 publications

Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria

Supramolecular Organization of the Respiratory Chain in Neurospora crassa Mitochondria

Specificity of interactions of the surface-active protonophore 2,4,6-trichloro-3-pentadecylphenol with artificial and mitochondrial membranes

On local coupling of electron transport and ATP-synthesis system in mitochondria. Theory and experiment

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