2020
DOI: 10.1093/mmy/myaa075
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On the lineage of Aspergillus fumigatus isolates in common laboratory use

Abstract: The origin of isolates routinely used by the community of Aspergillus fumigatus researchers is periodically a matter of intense discussion at our centre, as the construction of recombinant isolates have sometimes followed convoluted routes, the documentation describing their lineages is fragmented, and the nomenclature is confusing. As an aide memoir, not least for our own benefit, we submit the following account and tabulated list of strains (Table 1) in an effort to collate all of the relevant information in… Show more

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Cited by 66 publications
(81 citation statements)
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“…The A. fumigatus strain used in this study was CEA17ΔakuBKU80, which originated from the clinical isolate CBS 144–89 [ 11 , 12 ]. This strain was maintained on 2% malt-agar slants at ambient temperature.…”
Section: Methodsmentioning
confidence: 99%
“…The A. fumigatus strain used in this study was CEA17ΔakuBKU80, which originated from the clinical isolate CBS 144–89 [ 11 , 12 ]. This strain was maintained on 2% malt-agar slants at ambient temperature.…”
Section: Methodsmentioning
confidence: 99%
“…A. fumigatus strains wild-type strains Af293 and CEA10 along with MFIG001, a member of the CEA10 laboratory lineage lacking a functional ku80 gene, were used as the parental isolates for the transformations ( Bertuzzi et al, 2020 , Furukawa et al, 2020 ). Where the hygromycin resistance cassette was used as a selectable marker, it was amplified from the pAN7.1 plasmid (available from the Fungal Genetics Stock Centre) using primers detailed in the results section and Table S1.…”
Section: Methodsmentioning
confidence: 99%
“…Examination of virulence and virulence-related traits revealed the CAPA isolates often, but not always, had similar phenotypic profiles compared to reference A. fumigatus strains Af293 and CEA17 (which is a pyrG mutant derived from the reference strain A1163 (Bertuzzi et al, 2020)). For example, virulence in the Galleria moth model of fungal disease revealed strain heterogeneity among CAPA isolates, Af293, and CEA17 (p < 0.001; log-rank test; Figure 4A).…”
Section: Resultsmentioning
confidence: 99%
“…Examination of virulence and virulence-related traits revealed the CAPA isolates often, but not always, had similar phenotypic profiles compared to reference A. fumigatus strains Af293 and CEA17 (which is a pyrG mutant derived from the reference strain A1163 (Bertuzzi et al, 2020)).…”
Section: Capa Isolates Display Strain Heterogeneity In Virulence But mentioning
confidence: 99%