“…Purified mouse mammary tumor virus (MTV) from mammary tumors of (C3H/HeA X 020)F1 mice was a generous gift of Dr. P. Hageman. After incubation of the virus with pronase in the presence of SDS the viral RNA's were extracted with chloroform-phenol [21,22]. The 60 S component of RLV-RNA was isolated by centri-348 fugation in isokinetic glycerol gradients (15.0-30.2%, w/v).…”
“…Purified mouse mammary tumor virus (MTV) from mammary tumors of (C3H/HeA X 020)F1 mice was a generous gift of Dr. P. Hageman. After incubation of the virus with pronase in the presence of SDS the viral RNA's were extracted with chloroform-phenol [21,22]. The 60 S component of RLV-RNA was isolated by centri-348 fugation in isokinetic glycerol gradients (15.0-30.2%, w/v).…”
“…After homogenisation at 4°C the homogenate was deproteinised using the chloroform-phenol method in [6] and then precipitated by addition of NaCl to 0.1 M plus 2.5 vol. ethanol and incubated at -20°C overnight.…”
Section: Isolation and Translation Of Drosophila Rnamentioning
“…The RNA was subsequently fractionated on sucrose gradients; this procedure allowed us to deproteinize RNA by avoiding phenol extractions, which may lead to a selective loss of mRNA or of A-rich sequences ofmRNA [12,13]. The RNA precipitated from sucrose gradients was digested with pancreatic and T1 RNAase.…”
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