On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomes

Translation of oncogenic viral RNA and eukaryotic messenger RNA in the E. coli cell‐free system

“…Purified mouse mammary tumor virus (MTV) from mammary tumors of (C3H/HeA X 020)F1 mice was a generous gift of Dr. P. Hageman. After incubation of the virus with pronase in the presence of SDS the viral RNA's were extracted with chloroform-phenol [21,22]. The 60 S component of RLV-RNA was isolated by centri-348 fugation in isokinetic glycerol gradients (15.0-30.2%, w/v).…”

Analysis of the yolk proteins in Drosophila melanogaster

“…After homogenisation at 4°C the homogenate was deproteinised using the chloroform-phenol method in [6] and then precipitated by addition of NaCl to 0.1 M plus 2.5 vol. ethanol and incubated at -20°C overnight.…”

Presence of polyadenylic acid sequences in RNA of membrane‐bound polyribosomes

“…The RNA was subsequently fractionated on sucrose gradients; this procedure allowed us to deproteinize RNA by avoiding phenol extractions, which may lead to a selective loss of mRNA or of A-rich sequences ofmRNA [12,13]. The RNA precipitated from sucrose gradients was digested with pancreatic and T1 RNAase.…”