On the knowledge of the geographic variation of the satyrid Oeneis jutta (Hübner, [1806]) (Lepidoptera, Satyridae) in the Northeast of European Russia

et al. 2018

In the present study, specimens of Dendrolimus superans collected from South Korea suggest the presence of D. sibiricus, instead of D. superans. Comparisons of the wing morphology, female and male genitalia, 3′‐end region of the cytochrome c oxidase I (COI) gene sequence, and internal transcribed spacer (ITS) 2 sequence of the Korean specimens with those of the D. superans specimens from Japan consistently supported the presence of D. sibiricus in South Korea. Phylogenetic analyses of the concatenated sequences of COI and ITS2 from the available sequence types of D. sibiricus and D. superans, along with South Korean specimens, were conducted using the Bayesian inference (BI) and maximum likelihood (ML) methods. These phylogenetic analyses placed the South Korean specimens with the Russian D. sibiricus as an inclusive group, excluding the Japanese D. superans, indicating the distribution of D. sibiricus in South Korea. Nevertheless, D. superans formed a distinct group only by BI analysis (Bayesian posterior probabilities = 0.89), whereas D. sibiricus, including the South Korean samples, formed a distinct group only by the ML analysis (99%), suggesting a low genetic divergence between the two species.

Section: Methodsmentioning

confidence: 99%

DNA data and morphology suggest an occurrence of Dendrolimus sibiricus Tschetverikov, 1908 (Lepidoptera: Lasiocampidae) instead of D. superans Butler, 1877, in South Korea

Jeong

et al. 2018

“…We obtained data for K. consimilis females from the "Sphingidae Taxonomic Inventory" website for comparison (http://sphingidae.myspecies.info/taxonomy/term/1393/ media). External morphology including the male and female genitalia was examined (Brown 1955;Kulakova & Tatarinov 2011;Scoble 1992). These 34 specimens were all sequenced for their DNA barcoding sequence and nuclear EF-1α (Table 1).…”

Section: Specimens and Morphologymentioning

confidence: 99%

DNA data and morphology reveal the existence of Kentrochrysalis streckeri Staudinger, 1880 (Lepidoptera: Sphingidae) instead of K. consimilis in South Korea

Choi

et al. 2016

In the present study, we determined that specimens of Kentrochrysalis consimilis collected from South Korea were K. streckeri, rather than K. consimilis, based on morphology, DNA barcodes and nuclear elongation factor 1 alpha (EF-1α) sequences. The major morphological differences between K. streckeri and K. consimilis include the shape of forewing and hind-wing pattern elements and male and female genitalia. The DNA barcode analysis of the South Korean specimens and the Russiaoriginated K. streckeri showed a maximum sequence divergence of only 0.659% (4 bp), whereas that of the South Korean specimens and Japan-originated K. consimilis showed a minimum sequence divergence of 2.965% (18 bp), indicating that the Korean specimens are, in fact, K. streckeri and not K. consimilis. Phylogenetic analyses both by Bayesian inference and maximum likelihood methods strongly clustered the South Korean specimens and Russian K. streckeri into one group, excluding K. consimilis. The EF-1α-based sequence and phylogenetic analyses of the two species also supported data from the DNA barcode, indicating the distribution of K. streckeri in South Korea, instead of K. consimilis.

“…In this study, we performed a taxonomic review of the two species using major morphological characters, such as forewing length, which is one of the diagnostic characters for Oeneis (Kulakova & Tatarinov ) and eyespot pattern, which is frequently utilized for taxonomically identifying true butterflies including Satyrinae (Brown ). Furthermore, three mitochondrial genes (COI, ND6, and ND1) and one nuclear region (internal transcribed spacer 2, ITS2) were sequenced for molecular phylogenetic and population genetic analyses.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationships between Oeneis urda and O. mongolica (Nymphalidae: Lepidoptera)

Wan

et al. 2013

The species status of Oeneis urda (Eversmann) and O. mongolica (Oberthür) has been argued based on morphological characters. Reexamination of their major morphological characters has shown a slight differentiation in the two species. Sequences of three mitochondrial genes (COI, ND6, and ND1) and one nuclear region (internal transcribed spacer 2, ITS2) from two O. urda populations (Yangyang and Mt. Hanla) and one O. mongolica population (Uljin) were performed for phylogenetic and population genetic inferences. Sharing of identical sequences in the ND6 gene and ITS2, minimal sequence divergence in the COI and ND1 genes, and phylogenetically undividable sequence types in all mitochondrial genes and ITS2 suggest genetic continuity between the two species. Nevertheless, significant FST estimates (P < 0.05) were found for the COI gene in comparisons between Yangyang (O. urda) and Uljin (O. mongolica), between Yangyang (O. urda) and Mt. Hanla (O. urda), and between Uljin (O. mongolica) and Mt. Hanla (O. urda) populations. These FST estimates, along with other gene‐based analyses collectively suggest isolation of the two species at some point in the past, but incomplete separation between the two species on the mainland (Yangyang and Uljin) and biogeographically forced isolation of the O. urda population on Mt. Hanla collectively appear to complicate species status of these two species that were once further clearly separated.