On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer–Villiger oxidation process

Abstract

Efficient whole cell biotransformations, in particular microbial whole cell Baeyer-Villiger oxidation with molecular oxygen, demand comprehension and optimization of the process details involved. Optimal provision of oxygen and control of bioprocess parameters are pivotal for their success. The interrelation of cell density and oxygen supply in an in situ substrate feeding and product removal (SFPR) whole cell Baeyer-Villiger oxidation process was investigated in detail. Both parameters were optimized with respect to practical considerations. The outcome of this study supports a schematic process model, allows estimation of optimum process conditions and exploration of its limits.

Comparing the Stereoselective Biooxidation of Cyclobutanones by Recombinant Strains Expressing Bacterial Baeyer–Villiger Monooxygenases

“…Recent advances in the fermentation "up-scaling" of microbial Baeyer-Villiger oxidations clearly outline the applicability of this methodology to access valuable chiral building blocks on multi-gram scale. [23] Conclusions Chiral butyrolactone precursors for several natural products were obtained on a preparative scale by BVMO-mediated biooxygenation using recombinant whole cells. The available enzyme collection enabled access to enantiocomplementary products in several cases in moderate to excellent optical purities.…”

Overcoming the Gas–Liquid Mass Transfer of Oxygen by Coupling Photosynthetic Water Oxidation with Biocatalytic Oxyfunctionalization

“…Furthermore, the application of enzymes in whole cells, which is advantageous for oxygenases, suffers from a competition for O 2 between the target reaction and respiration 5. A technical solution for increasing the O 2 gas–liquid mass transfer rate under ambient conditions is the utilization of O 2 ‐enriched air 6. Yet, O 2 mass transfer is basically limiting the space–time yields of processes with high oxidation rates, especially in the production of bulk chemicals 1d, 5a, 7.…”

Biocatalytic Process Design and Reaction Engineering

“…Further optimization of reaction engineering parameters, such as the degree of conversion, selectivity, and specific productivity, require more work, but can lead to highly efficient synthetic procedures [59][60] . In addition to high space-time yield, reaction engineering and process intensification aim at complete conversion to the final product in order to avoid laborious product isolation operations [61][62][63] . Even with complete conversion, the final product also needs to be recovered and purified from the reaction system with high efficiency 64 .…”