On the influence of deacetylation process on the physicochemical characteristics of chitosan from squid chitin

Tolaimate, A.; Desbrières, Jacques; Rhazi, Mohammed; Alagui, Abdelhakim; Vincendon, M.; Vottero, P.

doi:10.1016/s0032-3861(99)00400-0

Cited by 301 publications

(206 citation statements)

References 27 publications

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“…250 mg portion of chitosan were dissolved in 10 ml of 0.30 M HCl and after being diluted to 50 ml with ultrapure water, it was titrated with 0.10 M NaOH. The consumed volume of NaOH solution, which corresponds to the amount of amine groups in chitosan, is obtained from the difference between two inflection points of acid-base titration (Tolaimate et al (2000)). …”

Section: Extraction Of Chitosanmentioning

confidence: 99%

Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells

et al. 2011

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a b s t r a c tIn this study, chitosan was extracted from Metapenaeus stebbingi shells. In order to determine physicochemical characteristics of the extracted chitosan, the yield, moisture and ash contents, degree of deacetylation, molecular weight, water and fat binding capacities, apparent viscosity and colour properties were measured using a variety of techniques including Fourier transform infrared spectroscopy, scanning electron microscopy and X-ray diffraction. In addition, the physicochemical characteristics of the chitosan extracted from M. stebbingi shells were compared to commercial chitosan. The degree of deacetylation was calculated by the titration method and elemental analysis. The molecular weight was determined by viscosimetric methods. The results of the study indicate that shrimp shells are a rich source of chitosan as 17.48% of the shell's dry weight is consisted of this material. Extracted chitosan exhibited a lower molecular weight, higher degree of deacetylation, higher viscosity and higher water and fat binding capacities compared to the commercial chitosan.

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Section: Extraction Of Chitosanmentioning

confidence: 99%

Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells

et al. 2011

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“…Functional groups of chitosan were characterized using FT-IR (Perkin-Elmer Spectrum 100). The elemental composition and degree of deacetylation of chitosan were determined by a LECO-CHNS-932 elemental analyzer (Mönchengladbach, Germany) and degree of deacetylation was calculated (20). Molecular weight of chitosan was determined using a viscosimetric method at the room temperature (19).…”

Section: Synthesis and Characterization Of Chitosanmentioning

confidence: 99%

Preparation, Characterization and Optimization of Chitosan Nanoparticles as Carrier for Immobilization of Thermophilic Recombinant Esterase

İlgü

Turan

Şanlı‐Mohamed

2011

Journal of Macromolecular Science, Part A

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Immobilization of biologically important molecules on myriad nano-sized materials has attracted great attention. Through this study, thermophilic esterase enzyme was obtained using recombinant DNA technology and purified applying one-step His-Select HF nickel affinity gel. The synthesis of chitosan was achieved from chitin by deacetylation process and degree of deacetylation was calculated as 89% by elemental analysis. Chitosan nanoparticles were prepared based on the ionic gelation of chitosan with tripolyphosphate anions. The physicochemical properties of the chitosan and chitosan nanoparticles were determined by several methods including SEM (Scanning Electron Microscopy), FT-IR (Fourier Transform Infrared Spectroscopy) and DLS (Dynamic Light Scattering). The morphology of chitosan nanoparticles was spherical and the nanospheres' average diameter was 75.3 nm. The purified recombinant esterase was immobilized efficiently by physical adsorption onto chitosan nanoparticles and effects of various immobilization conditions were investigated in details to develope highly cost-effective esterase as a biocatalyst to be utilized in biotechnological purposes. The optimal conditions of immobilization were determined as follows; 1.0 mg/mL of recombinant esterase was immobilized on 1.5 mg chitosan nanoparticles for 30 min at 60• C, pH 7.0 under 100 rpm stirring speed. Under optimized conditions, immobilized recombinant esterase activity yield was 88.5%. The physicochemical characterization of enzyme immobilized chitosan nanoparticles was analyzed by SEM, FT-IR and AFM (Atomic Force Microscopy).

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“…Elemental analysis and potentiometric titrimetry were used for the determination of the degree of deacetylation as reported in the literature [20,21]. The molecular weights of chitin and chitosan were determined by viscosimetric method which relates intrinsic viscosity of polymer to its molecular weight according to Mark-Houwink-Sakurada relation as described in the literature [21][22][23][24][25][26].…”

Section: Characterization Of Chitosanmentioning

confidence: 99%

Sorption of As(V) from waters using chitosan and chitosan-immobilized sodium silicate prior to atomic spectrometric determination

Boyacı

Eroğlu

Shahwan

2010

Talanta

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Keywords:As(V) As(III) Sorption Chitosan Chitosan-immobilized sodium silicate a b s t r a c t A natural biosorbent containing amine functional groups, chitosan, and a novel sorbent, chitosanimmobilized sodium silicate, were prepared and utilized for the selective sorption of As(V) from waters prior to its determination by atomic spectrometric techniques, namely, hydride generation atomic absorption spectrometry (HGAAS) and inductively coupled plasma mass spectrometry (ICP-MS). Chitosan was synthesized from chitin and sodium silicate was used as the immobilization matrix due to its straightforward synthesis. Through sequential sorption studies, it was shown that chitosan-immobilized sodium silicate has exhibited a better chemical stability than the chitosan itself which demonstrates the advantage of immobilization method. Both chitosan and chitosan-immobilized sodium silicate were shown to selectively adsorb As(V), arsenate, from waters at pH 3.0 at which neither chitin nor sodium silicate displayed any sorption towards As(V). The sorption of arsenate by chitosan is supposed to have electrostatic nature since pH of 3.0 is both the point at which the amino groups in chitosan are protonated and also the predominant form of As(V) is H 2 AsO 4 − . A pre-oxidation step is required if both As(III) and As(V) are to be determined. Desorption from the sorbents was realized with 1.0% (w/v) l-cysteine prepared in a pH 3.0 solution adjusted with HCl. Among the possible interfering species tested, only Te(IV) and Sb(III) were shown to cause a decrease in the sorption capacity especially at high interferant concentrations. High concentrations of Sb(III) also resulted in gas phase interference during hydride generation.The validity of the method was checked both via spike recovery experiments and also through the analysis of a standard reference material. Spike recovery tests were carried out with four different types of water; namely, ultra-pure, bottled drinking, tap, and sea water; and percent recovery values were found to be 114 (±4), 112 (±2), 43 (±4), and 0 (±1), respectively. It was concluded that the proposed methodology can be applied efficiently to low-to-medium ionic strength solutions, such as most drinking waters. The accuracy of the method was additionally investigated through the analysis of a standard reference material and a good correlation was found between the determined (26.6 ± 2.4 g L −1 ) and the certified (26.67 g L −1 ) value.

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On the influence of deacetylation process on the physicochemical characteristics of chitosan from squid chitin

Cited by 301 publications

References 27 publications

Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells

Physicochemical characterization of chitosan extracted from Metapenaeus stebbingi shells

Preparation, Characterization and Optimization of Chitosan Nanoparticles as Carrier for Immobilization of Thermophilic Recombinant Esterase

Sorption of As(V) from waters using chitosan and chitosan-immobilized sodium silicate prior to atomic spectrometric determination

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