On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Memmolo, Pasquale; Iannone, Maria; Ventre, Maurizio; Netti, Paolo A.; Fińizio, Andrea; Paturzo, Melania; Ferraro, Pietro

doi:10.1364/oe.20.028485

Cited by 65 publications

(44 citation statements)

References 20 publications

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“…In particular, we use the Angular Spectrum technique to calculate ) ; , ( d Q η ξ , while to obtain in-focus QPM we use a refocusing criterion based on the approximation of the Tamura coefficient (TC) [12], [13], where the minimum value in the region of interest of the QPM image corresponds to the reconstructed distance. From the sequence of in-focus QPM images, the diatom is first tracked, so as the QPMs are aligned in the same position, then the diatom is also oriented, so as the QPMs rotates in the same direction.…”

Section: Proposed Methodsmentioning

confidence: 99%

Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

et al. 2015

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Section: Proposed Methodsmentioning

confidence: 99%

Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

et al. 2015

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“…A holographic refocusing criterion, based on the Tamura coefficient method is used. 11,14 Such individual cell analysis is based on amplitude reconstructions of recorded holograms, in which cells appear in-focus when the Tamura coefficient is minimized.…”

Section: Digital Holographic Microscopy Trackingmentioning

confidence: 99%

Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

et al. 2015

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The Light Scattering Profile (LSP) of an individual cell provides a fast and accurate characterization of its morphological properties. By combining a camera-based small angle light scattering apparatus with a microfluidic-induced particle migration technique, it is possible to characterize cells in microfluidic flows. The scattering profile of an individual cell can be fully characterized by our optimized optical light collection system. Viscoelastic-induced particle migration by polyethylene oxide implemented in a low-cost microfluidic device composed of an alignment section and a measuring section opens the possibility of precise, label-free, individual cell analysis. We have studied living cells in microfluidic flows by our light scattering apparatus and by a Digital Holographic Microscope (DHM) system. Our DHM measurements provided an accurate 3D position tracking even in multiple cell conditions.

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“…This scanning approach, however, demands a relatively bulky and expensive imaging set-up. In contrast, recent advances in digital components and computational techniques have enabled powerful imaging methods, [1][2][3][4][5][6][7][8][9][10] and when these are combined with state-of-the-art image sensor technology, it has made lenses unnecessary in certain microscopic imaging tasks. [11][12][13][14][15][16][17] For example, by taking advantage of image sensor chips with large mega-pixels, small pixel pitch and low cost, lensfree holographic on-chip microscopy provides unique opportunities for achieving ultra-large SBP within a cost-effective and compact imaging design.…”

Section: Introductionmentioning

confidence: 99%

Synthetic aperture-based on-chip microscopy

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Wide field-of-view (FOV) and high-resolution imaging requires microscopy modalities to have large space-bandwidth products. Lensfree on-chip microscopy decouples resolution from FOV and can achieve a space-bandwidth product greater than one billion under unit magnification using state-of-the-art opto-electronic sensor chips and pixel super-resolution techniques. However, using vertical illumination, the effective numerical aperture (NA) that can be achieved with an on-chip microscope is limited by a poor signal-to-noise ratio (SNR) at high spatial frequencies and imaging artifacts that arise as a result of the relatively narrow acceptance angles of the sensor's pixels. Here, we report, for the first time, a synthetic aperture-based on-chip microscope in which the illumination angle is scanned across the surface of a dome to increase the effective NA of the reconstructed lensfree image to 1.4, achieving e.g., ,250-nm resolution at 700-nm wavelength under unit magnification. This synthetic aperture approach not only represents the largest NA achieved to date using an on-chip microscope but also enables color imaging of connected tissue samples, such as pathology slides, by achieving robust phase recovery without the need for multi-height scanning or any prior information about the sample. To validate the effectiveness of this synthetic aperture-based, partially coherent, holographic on-chip microscope, we have successfully imaged color-stained cancer tissue slides as well as unstained Papanicolaou smears across a very large FOV of 20.5 mm 2 . This compact on-chip microscope based on a synthetic aperture approach could be useful for various applications in medicine, physical sciences and engineering that demand high-resolution wide-field imaging. Keywords: computational imaging; lensfree microscopy; on-chip microscopy; synthetic aperture INTRODUCTIONWide field-of-view (FOV) and high-resolution imaging is crucial for various applications in biomedical and physical sciences. Such tasks demand microscopes to have large space-bandwidth products (SBPs) with minimal spatial aberrations that distort the utilization of the SBP of the imaging system. Conventional lens-based digital microscopes can achieve high-resolution imaging over a large FOV using mechanical scanning stages to capture multiple images from different parts of the specimen that are digitally stitched together. This scanning approach, however, demands a relatively bulky and expensive imaging set-up. In contrast, recent advances in digital components and computational techniques have enabled powerful imaging methods,

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On the holographic 3D tracking of in vitro cells characterized by a highly-morphological change

Cited by 65 publications

References 20 publications

Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

Full 3D morphology of diatoms flowing in a microfluidic channel by digital holographic microscopy

Cells characterization in microfluidic flows by small angle light scattering and 3D holographic technique

Synthetic aperture-based on-chip microscopy

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