On the functionality of a methionine sulfoxide reductase B from Trypanosoma cruzi

Arias, Diego Gustavo; Cabeza, Matías Sebastián; Echarren, María Laura; Faral-Tello, Paula; Iglesias, Alberto A.; Robello, Carlos; Guerrero, Sandra

doi:10.1016/j.freeradbiomed.2020.06.035

Cited by 7 publications

(4 citation statements)

References 79 publications

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“…Immunodetection experiments were adapted from a previously described method for T . cruzi [ 31 ]. Briefly, polyclonal antibodies against TvISGAf and TvISGAm were obtained by immunizing mice with 10 μg of purified recombinant protein at two-week intervals, as previously described [ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

Bontempi,

Arias,

Castro

et al. 2024

PLoS Negl Trop Dis

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Bovine trypanosomosis, caused by Trypanosoma vivax, currently affects cattle and has a significant economic impact in sub-Saharan Africa and South America. The development of new diagnostic antigens is essential to improve and refine existing methods. Our study evaluated the efficacy of two recombinant antigens in detecting specific antibodies in cattle. These antigens are derivatives of an invariant surface glycoprotein (ISG) from T. vivax. A fraction of a previously described antigen (TvY486_0045500), designated TvISGAf, from an African strain was evaluated, and a new ISG antigen from an American isolate, TvISGAm, was identified. The two antigens were expressed as fusion proteins in Escherichia coli: TvISGAf was fused to the MBP-His-tag, and TvISGAm was obtained as a His-tag fused protein. An ELISA evaluation was conducted using these antigens on 149 positive and 63 negative bovine samples. The diagnostic performance was enhanced by the use of a combination of both antigens (referred to as TvISG-based ELISA), achieving a sensitivity of 89.6% and specificity of 93.8%. Following the validation of the TvISG-based ELISA, the seroprevalence of T. vivax infection in 892 field samples from cattle in the central region of Argentina was determined. The mean seroprevalence of T. vivax was 53%, with variation ranging from 21% to 69% among the six departments studied. These results support the use of the TvISG ELISA as a valuable serological tool for the detection and monitoring of T. vivax infection in cattle. Furthermore, we report for the first time the seroprevalence of T. vivax in Argentina, which highlights the widespread endemic nature of the disease in the region. In order to effectively manage the increasing spread of T. vivax in the vast livestock production areas of South America, it is essential to implement consistent surveillance programs and to adopt preventive strategies.

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Section: Methodsmentioning

confidence: 99%

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

Bontempi,

Arias,

Castro

et al. 2024

PLoS Negl Trop Dis

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“…As previously described [51], rabbits were immunized with purified recombinant EhGLSP Proteins (recombinant or protein extracts) in SDS-PAGE gels and transferred onto nitrocellulose membranes using a Fast Blot, Semi-Dry Electrophoretic Transfer apparatus (Biometra GmbH). Western blotting was performed as previously described [41,52]. ECL western blotting detection reagents (Thermo Fisher Scientific) were used to visualize the bands.…”

Section: Protein Methods and Immunodetectionmentioning

confidence: 99%

On the occurrence of a glutaredoxin-like small protein in the anaerobic protozoan parasite Entamoeba histolytica

Birocco,

Gonzalez,

Guerrero

et al. 2023

Biochimica et Biophysica Acta (BBA) - General Subjects

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“…Beyond these specific regulatory switches at a global level, methionine oxidation may act as a sensor of cellular stress [1,2,[16][17][18][19][20][21][22][23] where protein incorporated MSox is accumulated, depleted, and regulated by the inducible system of Msr or related enzymes. The biological effects of Msr variation by knockout or knock-in have showed lifespan variation in flies related to antioxidant defense but results in mouse were equivocal, showing tissue specific mouse functions affected [24].…”

Section: Introductionmentioning

confidence: 99%

Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways

Lopes,

Schlatzer,

et al. 2024

Preprint

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Methionine oxidation to the sulfoxide form (MSox) is a poorly understood post-translational modification of proteins associated with nonspecific chemical oxidation from reactive oxygen species (ROS) whose chemistries are linked to various disease pathologies including neurodegeneration. Emerging evidence shows MSox site occupancy is in some cases under enzymatic regulatory control mediating cellular signaling including phosphorylation and/or calcium signaling, raising questions as to the speciation and functional nature of MSox across the proteome. The 5XFAD lineage of the C57BL/6 mouse has well-defined Alzheimer’s and aging states. Using this model, we analyzed age, sex and disease dependent MSox speciation in mouse hippocampus. In addition, we explore chemical stability and statistical variance of oxidized peptide signals to understand needed power for MSox based proteome studies. Our results identify mitochondrial and glycolytic pathway targets with increases in MSox with age as well as neuroinflammatory targets accumulating MSox with AD in proteome studies of mouse hippocampus. Further, the paper establishes a foundation for reproducible and rigorous experimental MSox-omics appropriate for biological discovery novel target identification, and biomarker analysis in ROS and other oxidation linked diseases.

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On the functionality of a methionine sulfoxide reductase B from Trypanosoma cruzi

Cited by 7 publications

References 79 publications

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

Improved serodiagnosis of Trypanosoma vivax infections in cattle reveals high infection rates in the livestock regions of Argentina

On the occurrence of a glutaredoxin-like small protein in the anaerobic protozoan parasite Entamoeba histolytica

Methionine Sulfoxide Speciation in Mouse Hippocampus Revealed by Global Proteomics Exhibits Age and Alzheimer’s Disease Dependent Changes Targeted to Mitochondrial and Glycolytic Pathways

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