On-the-fly investigation of XUV excited large molecular ions using a high harmonic generation light source

Abstract

We present experiments where extreme ultraviolet femtosecond light pulses are used to photoexcite large molecular ions at high internal energy. This is done by combining an electrospray ionization source and a mass spectrometer with a pulsed light source based on high harmonic generation. This allows one to study the interaction between high energy photons and mass selected ions in conditions that are accessible on large-scale facilities. We show that even without an ion trapping device, systems as large as a protein can be studied. We observe light induced dissociative ionization and proton migration in model systems such as reserpine, insulin and cytochrome c. These results offer new perspectives to perform time-resolved experiments with ultrashort pulses at the heart of the emerging field of attosecond chemistry.

Alterations in the odor profile of plants in cultivar mixtures affect aphid host-location behavior

“…Permutational multivariate analysis of variance (PERMANOVA) of the dissimilarity matrix was calculated using the adonis2 function (N permutations = 10.000). This was followed by pairwise comparisons between wheat monocultures and mixtures using the pairwise.perm.manova function of the ‘RVAideMemoire’ package ( Hervé et al., 2022 ). P-values were adjusted using the Bonferroni method.…”

Condensed Matter Systems Exposed to Radiation: Multiscale Theory, Simulations, and Experiment

“…From the experimental point of view, the interaction between attosecond pulses and complex biomolecules, such as an entire peptide or protein, is still in its infancy. 591,592 Current theoretical developments deal with the description of the light−matter interaction where the time-dependent light electric field is explicitly considered. The light first interacts with electronic degrees of freedom, and depending on its frequency outer valence, inner valence, and inner shell electrons must be included in the description.…”

“…Future Directions for the 5–10 Year Period : In order to develop attosecond and XFEL science in complex (bio)­molecules and clusters, it is crucial to connect ultrashort time scales where the interaction with light occurs and macroscopic observables where the consequence of the interaction is observed. From the experimental point of view, the interaction between attosecond pulses and complex biomolecules, such as an entire peptide or protein, is still in its infancy. , Current theoretical developments deal with the description of the light–matter interaction where the time-dependent light electric field is explicitly considered. The light first interacts with electronic degrees of freedom, and depending on its frequency outer valence, inner valence, and inner shell electrons must be included in the description.…”