On the existence of cardiomesenchymal stem cells

Navarro-Betancourt, José R.; Hernandez, Silvia C

doi:10.1016/j.mehy.2015.02.011

Cited by 5 publications

(2 citation statements)

References 69 publications

(73 reference statements)

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“…The dual ability of PL to both simultaneously expand and commit ADMSCs to the cardiac-like lineage could be also useful to overcome the low retention of engrafted MSCs observed within the cardiac tissue after in vivo injection [ 57 ], compensated by a higher number of injected cells. Besides, it has been extensively demonstrated that the cardiac differentiation potential of other different stem cell types (bone marrow derived c-kit + and monocytes, fibroblasts, or cardiomyocytes) is limited as they only provide either the angiogenic or the muscular component [ 58 ]. From this perspective, only cardiac resident progenitor cells could represent a better suitable option than MSCs [ 14 , 59 , 60 ]; however their scarcity and difficult retrievability in cardiac tissue limit their employment, albeit even combined cell therapy protocols have been suggested [ 61 ].…”

Section: Resultsmentioning

confidence: 99%

The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells

Siciliano

Chimenti

Bordin

et al. 2015

BioMed Research International

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Human adipose tissue-derived mesenchymal stem cells (ADMSCs) are considered eligible candidates for cardiovascular stem cell therapy applications due to their cardiac transdifferentiation potential and immunotolerance. Over the years, the in vitro culture of ADMSCs by platelet lysate (PL), a hemoderivate containing numerous growth factors and cytokines derived from platelet pools, has allowed achieving a safe and reproducible methodology to obtain high cell yield prior to clinical administration. Nevertheless, the biological properties of PL are still to be fully elucidated. In this brief report we show the potential ability of PL to induce a permissive state of cardiac-like transdifferentiation and to cause epigenetic modifications. RTPCR results indicate an upregulation of Cx43, SMA, c-kit, and Thy-1 confirmed by immunofluorescence staining, compared to standard cultures with foetal bovine serum. Moreover, PL-cultured ADMSCs exhibit a remarkable increase of both acetylated histones 3 and 4, with a patient-dependent time trend, and methylation at lysine 9 on histone 3 preceding the acetylation. Expression levels of p300 and SIRT-1, two major regulators of histone 3, are also upregulated after treatment with PL. In conclusion, PL could unravel novel biological properties beyond its routine employment in noncardiac applications, providing new insights into the plasticity of human ADMSCs.

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Section: Resultsmentioning

confidence: 99%

The Potential of GMP-Compliant Platelet Lysate to Induce a Permissive State for Cardiovascular Transdifferentiation in Human Mediastinal Adipose Tissue-Derived Mesenchymal Stem Cells

Siciliano

Chimenti

Bordin

et al. 2015

BioMed Research International

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“…They have also been reported to differentiate into other cell types, including skeletal and cardiac muscle cells [1, 2]. ADSCs are an ample source of adult stem cells that, unlike mesenchymal stem cells (MSCs) derived from bone marrow, can be procured by a simple and minimally invasive procedure [3].…”

Section: Introductionmentioning

confidence: 99%

Protective effects of alpha phenyl-tert-butyl nitrone and ascorbic acid in human adipose derived mesenchymal stem cells from differently aged donors

Johnson

Naaldijk

Hohaus

et al. 2016

Aging

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Adipose-derived mesenchymal stem cells (ADSCs) are multipotent stem cells that promote therapeutic effects and are frequently used in autologous applications. Little is known about how ADSCs respond to genotoxic stress and whether or not donor age affects DNA damage and repair. In this study, we used the comet assay to assess DNA damage and repair in human ADSCs derived from young (20-40 years), middle-aged (41-60 years), and older (61+ years) donors following treatment with H2O2 or UV light. Tail lengths in H2O2-treated ADSCs were substantially higher than the tail lengths in UV-treated ADSCs. After 30 minutes of treatment with H2O2, ADSCs preconditioned with alpha phenyl-tert-butyl nitrone (PBN) or ascorbic acid (AA) showed a significant reduction in % tail DNA. The majority of ADSCs treated with PBN or AA displayed low olive tail movements at various timepoints. In general and indicative of DNA repair, % tail length and % tail DNA peaked at 30 minutes and then decreased to near-control levels at the 2 hour and 4 hour timepoints. Differently aged ADSCs displayed comparable levels of DNA damage in the majority of these experiments, suggesting that the age of the donor does not affect the DNA damage response in cultured ADSCs.

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