On the efficacy of an Ascophyllum-based, soluble extract in association with standard plant growth regulators on the micropropagation of the agarophyte, Gracilaria blodgettii, from seaweed farms located at the northern entrance of the Panama Canal

Vega, Gloria Batista de; N, Carlos E. Ulloque; Hurtado, Anicia Q.; Cornish, Lynn; Critchley, Alan T.

doi:10.1007/s10811-020-02168-3

Cited by 7 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the use of AE in combination with PGR boosted micropropagation of the cultivated algae Kappaphycus alvarezii [ 34 , 40 , 41 , 42 , 43 ]. In a recent study Rayorath et al [ 35 ] studied the effect of two Ascophyllum nodosum extracts with and without combination with PGR on micropropagation of the tropical red alga Gracilaria blodgetti . The authors indicated that propagules of this alga treated with concentrations of AE as low as 0.1 mg L −1 could contribute to the successful production of vegetative propagules as seed stock.…”

Section: Discussionmentioning

confidence: 99%

“…It has also been reported for its use in in vitro micropropagation of algae. When combined with the plant growth regulators at lower concentrations, it boosted the micropropagation of Kappaphycus alvarezii [ 34 ] and of the agarophyte Gracilaria blodgetti [ 35 ]. However, apart from A. thaliana , which has been used as a rapid bioassay, to the best of our knowledge Ascophyllum nodosum extracts have never been tested for the micropropagation and regeneration of other higher plants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Application of Ascophyllum nodosum-Based Soluble Extract on Micropropagation and Regeneration of Nicotiana benthamiana and Prunus domestica

Faize

Burgos

et al. 2021

Plants

View full text Add to dashboard Cite

In the present study, the effect of a commercial extract of the seaweed Ascophyllum nodosum on in vitro micropropagation, shoot regeneration, and rhizoghenesis were studied in Nicotiana benthamiana and Prunus domestica. Results showed that the MS medium supplemented with various concentrations of the Ascophyllum extract (5, 10, 50, and 100 mg L−1) significantly enhanced the number of regenerated buds from N. benthamiana leaf discs to the conventional MS regenerating medium. Increases ranged from 3.5 to 6.5 times higher than the control. The effect of the Ascophyllum extract on N. benthamiana micropropagation was assessed through the measurement of some plant growth parameters. Results showed that the extract alone could not replace the micropropagation medium since shoot length, shoot diameter, root length, and leaf area were significantly reduced. However, its combination with a half-strength MS medium enhanced these parameters. Its effect was also evaluated on regeneration from plum hypocotyl slices. When added to the shoot regeneration medium without any plant growth regulators, the Ascophyllum extract alone could induce shoot regeneration. However, the percentage of bud regeneration and number of regenerated buds were lower than with the conventional shoot regeneration medium containing complete growth regulators. In contrast, the Ascophyllum extract drastically promoted rhizogenesis from plum hypocotyl slices. These results pave the way for the possible use of A. nodosum extracts in in vitro mass propagation of higher plants.

show abstract