On the Effects of UV‐C and pH on the Mechanical Behavior, Molecular Conformation and Cell Viability of Collagen‐Based Scaffold for Vascular Tissue Engineering

Achilli, Matteo; Lagueux, Jean; Mantovani, Diego

doi:10.1002/mabi.200900248

Cited by 44 publications

(42 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mechanical behavior was evaluated by considering the compressive modulus (CM), defined as the linear regression between 15 and 30% of strain, and the compressive strain energy (CSE), defined as the integral of the curve between 0 and 40% of strain. The gel prepared at pH 7, at an ionic strength corresponding to c4 and set at 37 °C was used as a control because, except for the concentration of collagen, it presented the same experimental conditions as the one previously studied in our laboratory [25].…”

Section: Methodsmentioning

confidence: 99%

“…This approach implies three experimental conditions: preparation of the gel solution with culture medium, gelation in the incubator and neutral pH [12,25]. These conditions fix the ionic strength, the pH and the temperature at which fibrillogenesis occurs and affect the size of the fibrils, the interactions between neighboring fibrils and, consequently, the type of microstructure and the mechanical properties of the scaffold.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

2010

Self Cite

View full text Add to dashboard Cite

Collagen gels have been widely studied for applications in tissue engineering because of their biological implications. Considering their use as scaffolds for vascular tissue engineering, the main limitation has always been related to their low mechanical properties. During the process of in vitro self-assembly, which leads to collagen gelation, the size of the fibrils, their chemical interactions, as well as the resulting microstructure are regulated by three main experimental conditions: pH, ionic strength and temperature. In this work, these three parameters were modulated in order to increase the mechanical properties of collagen gels. The effects on the gelation process were assessed by turbidimetric and scanning electron microscopy analyses. Turbidity measurements showed that gelation was affected by all three factors and scanning electron images confirmed that major changes occurred at the microstructural level. Mechanical tests showed that the compressive and tensile moduli increased by four-and three-fold, respectively, compared to the control. Finally, viability tests confirmed that these gels are suitable as scaffolds for cellular adhesion and proliferation.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, many skin changes like easy bruising which were believed to be a result of aging, are in fact related to prolonged UV exposure (Sionkowska, ). In addition, the UV‐collagen interaction affects the cell response, since it has been reported that cells that were cultured on UV–irradiated collagen‐based materials are influenced by UV irradiation parameters (Rajan et al, ; Achilli et al, ). Hence, due to the chronic exposure of human skin to sun light the clarification of the UV‐collagen interaction mechanisms is crucial.…”

Section: Introductionmentioning

confidence: 99%

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

et al. 2014

View full text Add to dashboard Cite

The objective of this paper was to investigate the influence of UV irradiation on collagen D-band periodicity by using the AFM imaging and nanoindentation methods. It is well known than UV irradiation is one of the main factors inducing destabilization of collagen molecules. Due to the human's skin chronic exposure to sun light, the research concerning the influence of UV radiation on collagen is of great interest. The impact of UV irradiation on collagen can be studied in nanoscale using Atomic Force Microscopy (AFM). AFM is a powerful tool as far as surface characterization is concerned, due to its ability to relate high resolution imaging with mechanical properties. Hence, high resolution images of individual collagen fibrils and load-displacement curves on the overlapping and gap regions, under various time intervals of UV exposure, were obtained. The results demonstrated that the UV rays affect the height level differences between the overlapping and gap regions. Under various time intervals of UV exposure, the height difference between overlaps and gaps reduced from ~3.7 nm to ~0.8 nm and the fibril diameters showed an average of 8-10% reduction. In addition, the irradiation influenced the mechanical properties of collagen fibrils. The Young's modulus values were reduced per 66% (overlaps) and 61% (gaps) compared to their initial values. The observed alterations on the structural and the mechanical properties of collagen fibrils are probably a consequence of the polypeptide chain scission due to the impact of the UV irradiation.

show abstract

“…As observed in Figure a1, FTIR analysis of collagen clearly exhibited the expected spectrum. Indeed, amide bands type I and II from collagen were at 1,656 and 1,554 cm −1 , respectively (Achilli, Lagueux, & Mantovani, ; Sionkowska et al, ). Based on the GNF structure, the characteristic bands from fluorocarbon chain were also detected (Figure a1), with a main band from CF 2 symmetric stretching at 1,201 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

A new composite hydrogel combining the biological properties of collagen with the mechanical properties of a supramolecular scaffold for bone tissue engineering

Maisani

Ziane

Ehret

et al. 2017

J Tissue Eng Regen Med

Self Cite

View full text Add to dashboard Cite

Tissue engineering is a promising alternative to autografts, allografts, or biomaterials to address the treatment of severe and large bone lesions. Classically, tissue engineering products associate a scaffold and cells and are implanted or injected into the lesion. These cells must be embedded in an appropriate biocompatible scaffold, which offers a favourable environment for their survival and differentiation. Here, we designed a composite hydrogel composed of collagen I, an extracellular matrix protein widely used in several therapeutic applications, which we associated with a physical hydrogel generated from a synthetic small amphiphilic molecule. This composite showed improved mechanical and biological characteristics as compared with gels obtained from each separate compound. Incorporation of the physical hydrogel prevented shrinkage of collagen and cell diffusion out of the gel and yielded a gel with a higher elastic modulus than those of gels obtained with each component alone. The composite hydrogel allowed cell adhesion and proliferation in vitro and long-term cell survival in vivo. Moreover, it promoted the differentiation of human adipose-derived stem cells in the absence of any osteogenic factors. In vivo, cells embedded in the composite gel and injected subcutaneously in immunodeficient mice produced lamellar osteoid tissue and differentiated into osteoblasts. This study points this new composite hydrogel as a promising scaffold for bone tissue engineering applications.

show abstract

On the Effects of UV‐C and pH on the Mechanical Behavior, Molecular Conformation and Cell Viability of Collagen‐Based Scaffold for Vascular Tissue Engineering

Cited by 44 publications

References 57 publications

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

Tailoring Mechanical Properties of Collagen-Based Scaffolds for Vascular Tissue Engineering: The Effects of pH, Temperature and Ionic Strength on Gelation

The effects of UV irradiation on collagen D-band revealed by atomic force microscopy

A new composite hydrogel combining the biological properties of collagen with the mechanical properties of a supramolecular scaffold for bone tissue engineering

Contact Info

Product

Resources

About