On the effect of substrate curvature on cell mechanics

Sanz-Herrera, José A.; Moreo, P.; García-Aznar, J.M.; Doblaré, M.

doi:10.1016/j.biomaterials.2009.08.053

Cited by 81 publications

(66 citation statements)

References 58 publications

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“…Similar results may also be achieved by increasing the width of microribbon blocks from 50-100 μ m to 100-200 μ m. Macroporosity also affects the average surface curvatures for cell adhesion, which may infl uence cell fate by modulating cytoskeleton tension in 3D. [ 14,15,27 ] Increasing evidence has highlighted the importance of matrix stiffness in dictating cell fates such as stem-cell differentiation and self-renewal. [28][29][30] One advantage of our microribbon-based scaffold design is the ability to decouple microscopic stiffness that cells sense from the macroscopic stiffness of the bulk scaffold.…”

Section: Doi: 101002/adma201304805mentioning

confidence: 77%

Photo‐crosslinkable PEG‐Based Microribbons for Forming 3D Macroporous Scaffolds with Decoupled Niche Properties

2013

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PEG-based microribbons are designed and fabricated as building blocks for constructing a 3D cell niche with independently tunable biochemical, mechanical, and topographical cues. This platform supports direct cell encapsulation, allows spatial patterning of biochemical cues, and may provide a valuable tool for facilitating the analyses of how interactive niche signaling regulates cell fate in three dimensions.

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Section: Doi: 101002/adma201304805mentioning

confidence: 77%

Photo‐crosslinkable PEG‐Based Microribbons for Forming 3D Macroporous Scaffolds with Decoupled Niche Properties

2013

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“…Size of macropores, on the other hand, influences nutrient diffusion and affects the surface curvature that cells sense within the scaffolds [18,23]. Conventional hydrogel scaffolds often present intertwined matrix stiffness and macroporosity; increased scaffold macroporosity is often achieved by reducing the concentration of hydrogel precursors, which spontaneously reduces the matrix stiffness cells sense [35].…”

Section: Gelatin Microribbons Enable the Independent Tuning Of Macropmentioning

confidence: 99%

“…Size of macropores can affect cell morphology via the surface curvature that the cells sense and may affect cell proliferation via the organization of cytoskeleton filaments [18,23]. This effect has been reported to influence many cell bioactivities, including cell proliferation and differentiation [32][33][34].…”

Section: Sizes Of Macropores Influence Cell Shapesmentioning

confidence: 99%

“…Prefabricated scaffolds with cell-sized microstructures, on the other hand, are widely used to control cell behaviors via ECM-mimicking microstructures, such as macropores [15][16][17][18][19], microchannels [20,21], and microfibrous textures [22]. These microarchitectures can promote desired cell fates by controlling cell morphology, supporting tissue deposition, and facilitating nutrient diffusion [16,18,19,23]. …”

Section: Introductionmentioning

confidence: 99%

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Engineering Mechanical, Biochemical, and Topographical Niche Cues by Photocrosslinkable, Microribbon-Like Hydrogels

Han

2016

Microscale Technologies for Cell Engineering

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“…Interestingly, it was found that cells tend to align either parallel or orthogonal to the direction of highest curvature, depending on the cell type. Inspired by these observations mechanical cell models have been proposed that suggest the importance of mechanical stress in the cytoskeleton for the detection of and response to curved structures [27,28].…”

Section: Introductionmentioning

confidence: 99%

Measuring cellular traction forces on non-planar substrates

et al. 2016

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Animal cells use traction forces to sense the mechanics and geometry of their environment. Measuring these traction forces requires a workflow combining cell experiments, image processing and force reconstruction based on elasticity theory. Such procedures have already been established mainly for planar substrates, in which case one can use the Green's function formalism. Here we introduce a workflow to measure traction forces of cardiac myofibroblasts on non-planar elastic substrates. Soft elastic substrates with a wave-like topology were micromoulded from polydimethylsiloxane and fluorescent marker beads were distributed homogeneously in the substrate. Using feature vector-based tracking of these marker beads, we first constructed a hexahedral mesh for the substrate. We then solved the direct elastic boundary volume problem on this mesh using the finite-element method. Using data simulations, we show that the traction forces can be reconstructed from the substrate deformations by solving the corresponding inverse problem with an L1-norm for the residue and an L2-norm for a zeroth-order Tikhonov regularization. Applying this procedure to the experimental data, we find that cardiac myofibroblast cells tend to align both their shapes and their forces with the long axis of the deformable wavy substrate.

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On the effect of substrate curvature on cell mechanics

Cited by 81 publications

References 58 publications

Photo‐crosslinkable PEG‐Based Microribbons for Forming 3D Macroporous Scaffolds with Decoupled Niche Properties

Photo‐crosslinkable PEG‐Based Microribbons for Forming 3D Macroporous Scaffolds with Decoupled Niche Properties

Engineering Mechanical, Biochemical, and Topographical Niche Cues by Photocrosslinkable, Microribbon-Like Hydrogels

Measuring cellular traction forces on non-planar substrates

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