On the droplet velocity and electrode lifetime of digital microfluidics: voltage actuation techniques and comparison

Dong, Cheng; Chen, Tianlan; Gao, Jie; Jia, Yanwei; Mak, Pui-In; Vai, Mang-I; Martins, Rui P.

doi:10.1007/s10404-014-1467-y

Cited by 36 publications

(14 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, if the flow rate decreases, droplets reside for a longer period within the main channel, which can be disadvantageous for fast sorting procedures (Figure S6, Supporting Information). It is possible to increase the applied potential (>126 V RMS ) to the electrodes (to increase the electrostatic force and work under higher flow rates), but this may induce dielectric breakdown, [ 77–79 ] followed by electrolysis or Joule heating which can ultimately lead to cell stress and to changes in genomic regulation in cells. [ 80 ] Hence, for gene‐editing experiments discussed below, we used flow rates below 45 nL s −1 to keep the droplets inside the trap while maintaining applied potentials below 126 V RMS .…”

Section: Resultsmentioning

confidence: 99%

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation

Samlali

Ahmadi

Quach

et al. 2020

Small

View full text Add to dashboard Cite

Generating a stable knockout cell line is a complex process that can take several months to complete. In this work, a microfluidic method that is capable of isolating single cells in droplets, selecting successful edited clones, and expansion of these isoclones is introduced. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co‐planar electrode system. In the hybrid microfluidics device, it is shown that single cells can be trapped and subsequently encapsulate them on demand into pL‐sized droplets. Furthermore, droplets containing single cells are either released, kept in the traps, or merged with other droplets by the application of an electric potential to the electrodes that is actuated through an in‐house user interface. This high precision control is used to successfully sort and recover single isoclones to establish monoclonal cell lines, which is demonstrated with a heterozygous NCI‐H1299 lung squamous cell population resulting from loss‐of‐function eGFP and RAF1 gene knockout transfections.

show abstract

Section: Resultsmentioning

confidence: 99%

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation

Samlali

Ahmadi

Quach

et al. 2020

Small

View full text Add to dashboard Cite

show abstract

“…The setup of the Digital Microuidic system was the same as previously described. [21][22][23] Briey, as shown in Fig. 2, there were four parts in the system: a DMF chip, an electronic control panel, a eld-programmable gate array (FPGA) board and a soware control program.…”

Section: Methodsmentioning

confidence: 99%

Adhesion promoter for a multi-dielectric-layer on a digital microfluidic chip

et al. 2015

Self Cite

View full text Add to dashboard Cite

A silane-based adhesion promoter suitable for a multi-dielectric-layer coating on a digital microfluidic chip is reported. It measures >100Â improvement in chip lifetime via transforming the bonding of the dielectric layers (Ta 2 O 5 and Parylene C) from nonspecific to chemical.The refined chip-fabrication protocol also allows low EWOD actuation voltages down to 5 V. † Electronic supplementary information (ESI) available: (1) a video demothe electrode lifetime testing. See

show abstract

“…† control, followed by multi-layer dielectric materials to enhance the EWOD force, thus reducing the threshold driving voltage. 34 This driving signal on the NMR sensing site is switched off during the NMR experiment to prevent interference on the sensitive NMR receiver. The specified sheet resistance of the ITO is 100 Ω sq −1 .…”

Section: Dmf Device and Electronicmentioning

confidence: 99%

“…S9 in the ESI †). 34 This driving signal on the NMR sensing site is switched off during the NMR experiment to prevent interference on the sensitive NMR receiver. To further enhance the portability of the system, an electronic boost converter is used to generate the square wave so as to circumvent the use of an extra high voltage supply.…”

Section: Dmf Device and Electronicmentioning

confidence: 99%

A palm-size μNMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis

Lei

Mak

Law

et al. 2015

Analyst

Self Cite

View full text Add to dashboard Cite

Herein, we describe a micro-nuclear magnetic resonance (μNMR) relaxometer miniaturized to palm-size and electronically automated for multi-step and multi-sample chemical/biological diagnosis. The co-integration of microfluidic and microelectronic technologies enables an association between the droplet managements and μNMR assays inside a portable sub-Tesla magnet (1.2 kg, 0.46 Tesla). Targets in unprocessed biological samples, captured by specific probe-decorated magnetic nanoparticles (NPs), can be sequentially quantified by their spin-spin relaxation time (T2) via multiplexed μNMR screening. Distinct droplet samples are operated by a digital microfluidic device that electronically manages the electrowetting-on-dielectric effects over an electrode array. Each electrode (3.5 × 3.5 mm(2)) is scanned with capacitive sensing to locate the distinct droplet samples in real time. A cross-domain-optimized butterfly-coil-input semiconductor transceiver transduces between magnetic and electrical signals to/from a sub-10 μL droplet sample for high-sensitivity μNMR screening. A temperature logger senses the ambient temperature (0 to 40 °C) and a backend processor calibrates the working frequency for the transmitter to precisely excite the protons. In our experiments, the μNMR relaxometer quantifies avidin using biotinylated Iron NPs (Φ: 30 nm, [Fe]: 0.5 mM) with a sensitivity of 0.2 μM. Auto-handling and identification of two targets (avidin and water) are demonstrated and completed within 2.2 min. This μNMR relaxometer holds promise for combinatorial chemical/biological diagnostic protocols using closed-loop electronic automation.

show abstract

On the droplet velocity and electrode lifetime of digital microfluidics: voltage actuation techniques and comparison

Cited by 36 publications

References 51 publications

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation

Adhesion promoter for a multi-dielectric-layer on a digital microfluidic chip

A palm-size μNMR relaxometer using a digital microfluidic device and a semiconductor transceiver for chemical/biological diagnosis

Contact Info

Product

Resources

About