On the Development of Reagent-free Conductive Nanocomposite Systems for the Modification of Printed Electrodes When Producing Glucose Biosensors

Arlyapov, V. A.; Kuznetsova, L. S.; Kharkova, Anna S.; Provotorova, Daria V.; Nenarochkina, E. D.; Kamanina, Olga A.; Мачулин, А. В.; Понаморева, О. Н.; Алферов, В. А.; Решетилов, А. Н.

doi:10.1134/s2635167622010025

Cited by 3 publications

(1 citation statement)

References 35 publications

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“…Composites with carbon nanostructures are usually used to create electroactive polymers [ 110 , 111 , 112 ], which, in turn, could be used to create electrochemical sensors, such as glucose [ 113 , 114 , 115 , 116 ], as well as whole-cell sensors [ 117 , 118 , 119 ], which make it possible to evaluate such important integral parameters as biochemical oxygen demand based on individual strains [ 120 , 121 , 122 ] or activated sludge [ 123 , 124 ] or assessment of toxic effects [ 125 ]. Such systems can be used, for example, to monitor the fermentation process [ 126 ] or toxicant removement [ 127 ].…”

Section: Tuning the Properties Of Composites Through The Introduction...mentioning

confidence: 99%

On the Use of Polymer-Based Composites for the Creation of Optical Sensors: A Review

2022

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Polymers are widely used in many areas, but often their individual properties are not sufficient for use in certain applications. One of the solutions is the creation of polymer-based composites and nanocomposites. In such materials, in order to improve their properties, nanoscale particles (at least in one dimension) are dispersed in the polymer matrix. These properties include increased mechanical strength and durability, the ability to create a developed inner surface, adjustable thermal and electrical conductivity, and many others. The materials created can have a wide range of applications, such as biomimetic materials and technologies, smart materials, renewable energy sources, packaging, etc. This article reviews the usage of composites as a matrix for the optical sensors and biosensors. It highlights several methods that have been used to enhance performance and properties by optimizing the filler. It shows the main methods of combining indicator dyes with the material of the sensor matrix. Furthermore, the role of co-fillers or a hybrid filler in a polymer composite system is discussed, revealing the great potential and prospect of such matrixes in the field of fine properties tuning for advanced applications.

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Section: Tuning the Properties Of Composites Through The Introduction...mentioning

confidence: 99%

On the Use of Polymer-Based Composites for the Creation of Optical Sensors: A Review

2022

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Development of Nanocomposite Materials Based on Conductive Polymers for Using in Glucose Biosensor

et al. 2022

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Electropolymerized neutral red, thionine, and aniline were used as part of hybrid nanocomposite conductive polymers, to create an amperometric reagent-less biosensor for glucose determination. The structure of the obtained polymers was studied using infrared (IR) spectroscopy and scanning electron microscopy. Electrochemical characteristics were studied by cyclic voltammetry and impedance spectroscopy. It was shown that, from the point of view of both the rate of electron transfer to the electrode, and the rate of interaction with the active center of glucose oxidase (GOx), the most promising is a new nanocomposite based on poly(neutral red) (pNR) and thermally expanded graphite (TEG). The sensor based on the created nanocomposite material is characterized by a sensitivity of 1000 ± 200 nA × dm3/mmol; the lower limit of the determined glucose concentrations is 0.006 mmol/L. The glucose biosensor based on this nanocomposite was characterized by a high correlation (R2 = 0.9828) with the results of determining the glucose content in human blood using the standard method. Statistical analysis did not reveal any deviations of the results obtained using this biosensor and the reference method. Therefore, the developed biosensor can be used as an alternative to the standard analysis method and as a prototype for creating sensitive and accurate glucometers, as well as biosensors to assess other metabolites.

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Bioanalytical System for Determining the Phenol Index Based on Pseudomonas putida BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer “Bovine Serum Albumin–Ferrocene–Carbon Nanotubes”

et al. 2022

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The possibility of using the microorganisms Pseudomonas sp. 7p-81, Pseudomonas putida BS394(pBS216), Rhodococcus erythropolis s67, Rhodococcus pyridinivorans 5Ap, Rhodococcus erythropolis X5, Rhodococcus pyridinivorans F5 and Pseudomonas veronii DSM 11331T as the basis of a biosensor for the phenol index to assess water environments was studied. The adaptation of microorganisms to phenol during growth was carried out to increase the selectivity of the analytical system. The most promising microorganisms for biosensor formation were the bacteria P. putida BS394(pBS216). Cells were immobilized in redox-active polymers based on bovine serum albumin modified by ferrocenecarboxaldehyde and based on a composite with a carbon nanotube to increase sensitivity. The rate constants of the interaction of the redox-active polymer and the composite based on it with the biomaterial were 193.8 and 502.8 dm3/(g·s) respectively. For the biosensor created using hydrogel bovine serum albumin-ferrocene-carbon nanotubes, the lower limit of the determined phenol concentrations was 1 × 10−3 mg/dm3, the sensitivity coefficient was (5.8 ± 0.2)∙10−3 μA·dm3/mg, Michaelis constant KM = 230 mg/dm3, the maximum rate of the enzymatic reaction Rmax = 217 µA and the long-term stability of the bioanalyzer was 11 days. As a result of approbation, it was found that the urban water phenol content differed insignificantly, measured by creating a biosensor and using the standard photometric method.

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On the Development of Reagent-free Conductive Nanocomposite Systems for the Modification of Printed Electrodes When Producing Glucose Biosensors

Cited by 3 publications

References 35 publications

On the Use of Polymer-Based Composites for the Creation of Optical Sensors: A Review

On the Use of Polymer-Based Composites for the Creation of Optical Sensors: A Review

Development of Nanocomposite Materials Based on Conductive Polymers for Using in Glucose Biosensor

Bioanalytical System for Determining the Phenol Index Based on Pseudomonas putida BS394(pBS216) Bacteria Immobilized in a Redox-Active Biocompatible Composite Polymer “Bovine Serum Albumin–Ferrocene–Carbon Nanotubes”

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