It has been known for a long time that unblanched vegetables deteriorate in quality when held in frozen storage for extended periods of time. Rancidity of the lipid matter is one of the primary causes of off-flavor in unblanched peas which had been stored at 0°F. (-17.8"C.) (5). Large increases in acid numbers together with increases in peroxide numbers were observed in the lipids extracted from raw peas after several years of frozen storage. The main increase in acid number occurred during the first year of storage (8). Similar changes were noted in the lipids extracted from unblanched sweet corn, snap beans, and other vegetables after extended periods of storage at 0°F. (-17.8"C.) ( 4 ) .I n order to extend the knowledge of the changes which take place in such material, the present study was undertaken to determine the length of time that unblanched vegetables could be held in frozen storage before these undesirable changes could be detected, and to determine the course of the development of the acids and peroxides during the period of storage.
MATERIALS AND METHODSThomas Laxton peas, Wade snap beans, and Golden Cross sweet corn were grown on the Experiment Station farm and were harvested, processed, and stored, as previously described (4, 5 ) .Quality changes were measured both chemically and by means of a taste panel. Samples from each lot were taken for immediate taste test a t the time of harvest, and at regular intervals during storage, until obvious deterioration in quality was definitely established. A t the same time, samples were dried by 1yophiIization and were extracted for 48 hours with peroxide-free anhydrous ethyl ether to obtain the crude lipid material, as described previously ( 5 ) . This work was started before it was determined that a 24-hour extraction period was sufficient to effect the extraction of the crude lipid (4), but was continued on the 48-hour basis to keep the conditions uniform. The chemical determinations were continued a t regular but lengthening intervals throughout the storage period. A final subjective and chemical examination of the 1952 samples was conducted after 2 years of storage. Chemical methods used were described previously (4, 5).