On the Determination of the Hold-Up Time in Reversed Phase Liquid Chromatography

Berendsen, Gert E.; Schoenmakers, Peter J.; Galan, L. de; Vigh, Gyula; Varga‐Puchony, Z.; Inczédy, J.

doi:10.1080/01483918008064759

Cited by 174 publications

(56 citation statements)

References 26 publications

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“…Indeed, it has been previously shown that the use of small ionic species (NaNO 3 ) as retention time of unretained compound markers lead to underestimated t M values which represent an exclusion volume instead of the effective column dead volume or retention time of unretained compound and the retention time of the inorganic salts increases with increasing sample concentration, or amount [11,12].…”

Section: Resultsmentioning

confidence: 98%

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Retention Time of Unretained Compound Calculation and Determination of Retention Indices of Some Monosubstituted Benzenes Using a Multiparametric Method in Binary, Ternary and Quaternary Solvent RP-LC Systems

et al. 2011

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In reversed phase liquid chromatography (RP-LC), the validity of a multiparametric (MP) non-linear least-squares regression iterative method has been evaluated for 14 different aqueous mobile phases modified with one, two or three organic solvents [acetonitrile (ACN), methanol (MeOH), or tetrahydrofuran (THF)] for calculating the retention time of unretained compound t M and the regression parameter (slope b), based on the use of alkan-2-ones and alkyl aryl ketones homologous series. The determination of t M and b has been studied for eight binary (ACN-H 2 O or MeOH-H 2 O), 3 ternary (ACNMeOH-H 2 O) and 3 quaternary (ACN-MeOH-THF-H 2 O) mobile phase systems on an Omnispher C 18 column. The multiparametric calculated t M and b values were compared with those obtained by Guardino's, and Grobler's methods. The MP retention indices (RI) of ten monosubstituted benzenes with different functionality (hydroxyl, carbonyl, nitro, etc.) based on the alkan-2-ones retention index standards have been determined and compared for the different mobile phase compositions studied. The influence of organic modifier type, the nature of mobile phase system and water content on the variation of retention parameter studied in this work were discussed.Keywords Reversed phase-liquid chromatography Á Binary, ternary and quaternary eluent systems Á Multiparametric least-squares adjustment Á Retention time of unretained compound and Retention indices Á Homologous series and monosubstituted benzenes

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Section: Resultsmentioning

confidence: 98%

“…Several mathematical procedures for the evaluation of retention time of unretained compound t M and the retention indices (RI) have been developed in GC [11,12] and reviewed [13]. Some of these procedures have also been applied to the determination of retention parameter in RP-LC [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Retention Time of Unretained Compound Calculation and Determination of Retention Indices of Some Monosubstituted Benzenes Using a Multiparametric Method in Binary, Ternary and Quaternary Solvent RP-LC Systems

et al. 2011

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“…As can be expected from all the sets of data from shown that the use of small ionic species as dead time markers lead to underestimated t M values which represent an exclusion volume instead of the effective column dead volume or dead time [7].…”

Section: Effect Of Mobile Phase Compositionmentioning

confidence: 92%

“…In the practice of liquid chromatography, numerous solutes (e.g., mobile phase components [7,21], isotopically labeled mobile phase components [7,8], as well as radiolabeled components of the eluent [22], ionic [6,7] and non-ionic [23,24] species) have been used as dead time markers, but the use of such compounds was termed as "clearly dangerous" by Berendsen et al [7].…”

Section: Introductionmentioning

confidence: 99%

“…This is because a reliable estimation of this quantity is essential for the correct calculation of the retention factor, k [3 -6], which serves as the fundamental parameter for the comparison of retention data and for interpretation of the physico-chemical phenomena taking place within a chromatographic column [1,7]. It warrants mention that the thermodynamic information involved in the retention factor can be correctly extracted only from precisely determined k values [1,8].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Dead Time Calculation in Reversed-Phase Liquid Chromatography Using a Multiparametric Mathematical Method

Didaoui

Touabet

Ahmed

et al. 1999

J. High Resol. Chromatogr.

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In reversed phase liquid chromatography (RP‐HPLC), the validity of a multiparametric non‐linear least‐squares regression iterative method for the determination of the column dead time tM and the regression parameter (slope b), based on the use of alkan‐2‐ones, alkyl aryl ketones, and 1‐nitroalkanes has been evaluated. The determination of tM and b has been extensively studied for two mobile phase systems acetonitrile‐water and methanol‐water on seven octadecyl‐C18 and one octyl C8 analytical columns. The calculated tM and b values were compared with those obtained by Guardino's and Grobler's methods. The influence exerted thereon by the nature of the homologous series, the mobile phase composition, and the packing materials were investigated.

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Elevated temperature liquid chromatography using reversed-phase packed capillary columns

1997

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On the Determination of the Hold-Up Time in Reversed Phase Liquid Chromatography

Cited by 174 publications

References 26 publications

Retention Time of Unretained Compound Calculation and Determination of Retention Indices of Some Monosubstituted Benzenes Using a Multiparametric Method in Binary, Ternary and Quaternary Solvent RP-LC Systems

Retention Time of Unretained Compound Calculation and Determination of Retention Indices of Some Monosubstituted Benzenes Using a Multiparametric Method in Binary, Ternary and Quaternary Solvent RP-LC Systems

Evaluation of Dead Time Calculation in Reversed-Phase Liquid Chromatography Using a Multiparametric Mathematical Method

Elevated temperature liquid chromatography using reversed-phase packed capillary columns

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