“…It is not established whether TBP association at TATA-less promoters drives similar degree of distortion (257). Mutational analyses have shown that single nucleotide alterations within the canonical TATA sequence can severely decrease the TBP-DNA binding affinity, suggesting that degenerate TATA-like sequences and TATA-less promoters may exhibit lower TBP affinity and, perhaps, lower degree of TBP intercalation as well (257,258). And while TAFs and TFIIA may compensate for this by stabilizing TBP onto the DNA, the possibility of differential DNA distortion based on the underlying TBP-bound sequence may be reflective of the dynamics of TBP turnover in post-initiation stage.…”