On the bright side of a forest pest-the metabolic potential of bark beetles' bacterial associates

Fabryová, Anna; Kostovčík, Martin; Díez-Méndez, Alexandra; Jiménez‐Gómez, Alejandro; Celador-Lera, Lorena; Saati‐Santamaría, Zaki; Sechovcová, Hana; Menéndez, Esther; Kolařík, Miroslav; García‐Fraile, Paula

doi:10.1016/j.scitotenv.2017.11.074

Cited by 28 publications

(44 citation statements)

References 33 publications

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“…Bacteria were implicated in the modification of mating systems, degradation of plant compounds, synthesis of vitamins and amino acids in different insect species (Sudakaran et al, 2017, Werren et al, 2008, Nikoh et al, 2014. On the contrary, a limited number of studies investigated the role of bacterial microorganisms in promoting ecological specialization and refining metabolic capacity in Scolytinae and Platypodinae (Fabryova et al, 2017, Hernandez-Garcia et al, 2017, Popa et al, 2012.…”

Section: Next Frontiers In Bark and Ambrosia Beetles Phylogenetics Anmentioning

confidence: 99%

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Pistone

Gohli

Jordal

2017

Systematic Entomology

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Section: Next Frontiers In Bark and Ambrosia Beetles Phylogenetics Anmentioning

confidence: 99%

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Pistone

Gohli

Jordal

2017

Systematic Entomology

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“…The list of bacterial isolates analyzed in this work, as well as their identification based on their partial (≈1,400 bp) 16S rRNA sequence, is presented in Table 1 . The list includes the isolates from I. acuminatus and P. bidentatus , obtained in this study, and the ones from the bark beetles C. piceae and P. pityographus obtained in a study that tested their capacity to degrade plant cells (Fabryová et al, 2018 ).…”

Section: Resultsmentioning

confidence: 99%

“… Ref, Isolated previously in Fabryová et al ( 2018 ); N, Novel isolates; PP, Pityophthorus pityographus; CP, Cryphalus piceae; IA, Ips acuminatus; PB, Pityogenes bidentatus; Ps, Pinus sylvestris; Aal, Abies alba; +, It does exist inhibition; −, It does not exist inhibition; w, weakly inhibition. Bold values are for those strains selected for genome sequencing .…”

Section: Resultsmentioning

confidence: 99%

“…Bacterial strains used in this study were isolated from adult bark beetles of several species - Ips acuminatus, Cryphalus piceae, Pityophthorus pityographus , and Pityogenes bidentatus (all Coleoptera, Scolytinae )-. The isolation of the bacterial associates of C. piceae and P. pityographus has been previously described in Fabryová et al ( 2018 ). The newly obtained bacteria isolated in this study were obtained from I. acuminatus and P. bidentatus beetles, extracted from branches from Pinus sylvestris exhibiting the typical boring holes of bark beetles which were collected in May 2016 in Stará Boleslav, Czech Republic (coordinates: 50°12′59.5″N, 14°41′58.4″E).…”

Section: Methodsmentioning

confidence: 99%

“…Almost complete (~1,400 bp) 16S rRNA sequences were compared with those available in GenBank using the BLASTn program (Altschul et al, 1990 ) and EzTaxon tool (Kim et al, 2012 ). The remaining 40 strains used in the antimicrobial screening were identified as detailed by Fabryová et al ( 2018 ).…”

Section: Methodsmentioning

confidence: 99%

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Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

et al. 2018

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Antimicrobial resistance is a worldwide problem that threatens the effectiveness of treatments for microbial infection. Consequently, it is essential to study unexplored niches that can serve for the isolation of new microbial strains able to produce antimicrobial compounds to develop new drugs. Bark beetles live in phloem of host trees and establish symbioses with microorganisms that provide them with nutrients. In addition, some of their associated bacteria play a role in the beetle protection by producing substances that inhibit antagonists. In this study the capacity of several bacterial strains, isolated from the bark beetles Ips acuminatus, Pityophthorus pityographus Cryphalus piceae, and Pityogenes bidentatus, to produce antimicrobial compounds was analyzed. Several isolates exhibited the capacity to inhibit Gram-positive and Gram-negative bacteria, as well as fungi. The genome sequence analysis of three Pseudomonas isolates predicted the presence of several gene clusters implicated in the production of already described antimicrobials and moreover, the low similarity of some of these clusters with those previously described, suggests that they encode new undescribed substances, which may be useful for developing new antimicrobial agents. Moreover, these bacteria appear to have genetic machinery for producing antitumoral and antiviral substances. Finally, the strain IA19T showed to represent a new species of the genus Pseudomonas. The 16S rRNA gene sequence analysis showed that its most closely related species include Pseudomonas lutea, Pseudomonas graminis, Pseudomonas abietaniphila and Pseudomonas alkylphenolica, with 98.6, 98.5 98.4, and 98.4% identity, respectively. MLSA of the housekeeping genes gyrB, rpoB, and rpoD confirmed that strain IA19T clearly separates from its closest related species. Average nucleotide identity between strains IA19T and P. abietaniphila ATCC 700689T, P. graminis DSM 11363T, P. alkylphenolica KL28T and P. lutea DSM 17257T were 85.3, 80.2, 79.0, and 72.1%, respectively. Growth occurs at 4-37°C and pH 6.5-8. Optimal growth occurs at 28°C, pH 7–8 and up to 2.5% NaCl. Respiratory ubiquinones are Q9 (97%) and Q8 (3%). C16:0 and in summed feature 3 are the main fatty acids. Based on genotypic, phenotypic and chemotaxonomic characteristics, the description of Pseudomonas bohemica sp. nov. has been proposed. The type strain is IA19T (=CECT 9403T = LMG 30182T).

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Associations Between Bark Beetles and Pseudomonas

Saati‐Santamaría

Rivas

Kolařík

et al. 2021

Developmental Biology in Prokaryotes and Lower Eukaryotes

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On the bright side of a forest pest-the metabolic potential of bark beetles' bacterial associates

Cited by 28 publications

References 33 publications

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Molecular phylogeny of bark and ambrosia beetles (Curculionidae: Scolytinae) based on 18 molecular markers

Discovery of Phloeophagus Beetles as a Source of Pseudomonas Strains That Produce Potentially New Bioactive Substances and Description of Pseudomonas bohemica sp. nov.

Associations Between Bark Beetles and Pseudomonas

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