On the Asymmetry of Rna Synthesis in Vivo

Tocchini‐Valentini, Glauco P.; Stodolsky, Marvin; Aurisicchio, A.; Sarnat, M.; Graziosi, Francesca; Weiss, Samuel B.; Geiduschek, E. Peter

doi:10.1073/pnas.50.5.935

Cited by 80 publications

(15 citation statements)

References 20 publications

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“…The earliest demonstrations of selective transcription in vivo and in vitro used DNA-RNA hybridization to demonstrate that RNA transcripts anneal preferably to one of the two DNA strands for a given genetic region (129)(130)(131)(132). The technique of DNA-RNA hybridization has continued to be a major tool for studying the origins of RNA transcripts ; a large variety of techniques based on hybridization have been devised.…”

Section: Measurement Of Selective Transcription In Vitromentioning

confidence: 99%

“…Since many phages use only one strand of the chromosome (129,131,132,136) or restrict transcription of different strands to different temporal classes of RNA (137)(138)(139), selectivity is often monitored simply by following the asymmetry transcript annealing to the separate DNA strands. Procedures have been described that allow separation of the DNA strands for a large variety of bacterial and phage species (140).…”

Section: Measurement Of Selective Transcription In Vitromentioning

confidence: 99%

See 1 more Smart Citation

The Selectivity of Transcription

Chamberlin¹

1974

Annu. Rev. Biochem.

529

213

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Section: Measurement Of Selective Transcription In Vitromentioning

confidence: 99%

Section: Measurement Of Selective Transcription In Vitromentioning

confidence: 99%

The Selectivity of Transcription

Chamberlin¹

1974

Annu. Rev. Biochem.

529

213

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“…As a control, the latter could be shown to form DNA:RNA hybrids with self-complementary RNA synthesized in vitro. The 32 P distribution among nucleotides of ␣ RNA synthesized in the infected cell also corresponded nicely with the composition of L-strand ␣ DNA (37).…”

Section: Rna Polymerasementioning

confidence: 76%

An Introduction to Transcription and Gene Regulation

Geiduschek

2010

Journal of Biological Chemistry

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“…Finally, three different groups (231)(232)(233)(234) have employed one-and two stranded DNA from bacterial viruses in direct hybridization experiments. The phages used were cfJX 174 (234), where the phage particle yields one stranded DNA and two-stranded phage DNA can be isolated from infected E. coli cells, and the B. subtilis phages IX (23 1, 232) and SP 8 (233) , where the two strands of denatured phage DNA can be separated by density gradient centrifugation.…”

Section: Rna Polymerasementioning

confidence: 99%

Metabolism of Nucleic Acids (Macromolecular DNA and RNA)

Elson¹

1965

Annu. Rev. Biochem.

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Since it is manifestly impossible to cover the entire field of nucleic acid metabolism in one article, I have restricted this review to recent work on enzymes which have to do with macromolecular nucleic acids. Sections are included on the synthetic enzymes DNA polymerase and RNA polymerase and on enzymes which alter the nucleic acids in their macromolecular state, namely, the DNA and RNA methylases. The concluding section deals with degradative enzymes, chiefly RNase, DNase, and polynucleotide phosphoryl ase, but in a much more cursory manner. The review is based on an exten sive, but certainly not complete, coverage of the recent literature. References 1 through 10 are reviews, books, special collections of articles, and critical papers which deal with various aspects of the subject matter of this review. ENZYMES WHICH SYNTHESIZE NUCLEIC ACIDSThe DNA polymerases and RNA polymerases catalyze the synthesis of polynucleotides by the complementary copying of pre-existing polynucleo tides. The precursors are the appropriate nucleoside triphosphates, and a bivalent cation is required (11-16). During the period under review, there have appeared no serious reasons to doubt that these are the principal, and quite possibly the only, enzymes which catalyze the synthesis de novo of DNA and RNA in nature. Beyond this, a great deal remains to be eluci dated, and much of the recent work has dealt with such matters as the chem ical and physical nature of the polynucleotide templates or primers and prod ucts, details of the reaction mechanisms, differences between the reactions in vivo and in vitro, the association of the enzymes with other cell components, and so forth.DNA POLYMERASEThe location of DNA polymerase in the cell.-In bacterial extracts DNA polymerase has been observed to sediment together with DNA (e.g., 17, 18) . In higher organisms, however, a number of studies had indicated that the enzyme is actually located outside the cell nucleus. In view of the unexpected nature of these findings, the matter has been pursued in several laboratories; and reports have now appeared which provide evidence that at least some of

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On the Asymmetry of Rna Synthesis in Vivo

Cited by 80 publications

References 20 publications

The Selectivity of Transcription

The Selectivity of Transcription

An Introduction to Transcription and Gene Regulation

Metabolism of Nucleic Acids (Macromolecular DNA and RNA)

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