On the application of supported bilayers as receptive layers for biosensors with electrical detection

Stelzle, Martin; Weissmueller, G.; Sackmann, E.

doi:10.1021/j100114a025

Cited by 275 publications

(218 citation statements)

References 5 publications

(11 reference statements)

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“…Here we focus on detection of DNA and proteins. Among impedance sensor applications not discussed here are small molecule sensors (e.g., [21][22][23][24]), cell-based biosensors (e.g., [25][26][27][28]), and lipid bilayer sensors (e.g., [29,30]). …”

Section: Introductionmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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Impedance biosensors are a class of electrical biosensors that show promise for point-of-care and other applications due to low cost, ease of miniaturization, and label-free operation. Unlabeled DNA and protein targets can be detected by monitoring changes in surface impedance when a target molecule binds to an immobilized probe. The affinity capture step leads to challenges shared by all label-free affinity biosensors; these challenges are discussed along with others unique to impedance readout. Various possible mechanisms for impedance change upon target binding are discussed. We critically summarize accomplishments of past label-free impedance biosensors and identify areas for future research.

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Section: Introductionmentioning

confidence: 99%

Label‐Free Impedance Biosensors: Opportunities and Challenges

2007

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“…Thus, despite the number of publications in recent years on SLB formation and the in-depth knowledge generated, there are no similar investigations on how SLBs based on, e.g., bacterial lipids, can be self-assembled from liposomes' unfunctionalized biosensor substrates. Some previous attempts have been made toward creating SLB from liposomes including some relevant lipids for bacteria [29][30][31][32][33][34][35][36][37][38] or even close to complete bacterial lipid mixtures. 27,28,30,31,39,40 However, these studies have mostly used hydrophobic or polycationic polymers, which strongly promote liposome rupture in order to improve SLB yield, which resulted in incomplete and probably strongly pinned SLB, or else are low coverage preparations of SLB and vesicles on mica and glass substrates There is also lacking an investigation of how these membranes are formed, and thus little knowledge for improving coverage and reproducibility of the SLB.…”

Section: Introductionmentioning

confidence: 99%

Formation of supported bacterial lipid membrane mimics

Merz¹,

Knoll²,

Textor³

et al. 2008

Biointerphases

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In recent years, a large effort has been spent on advancing the understanding of how surface-supported membranes are formed through vesicle fusion. The aim is to find simple model systems for investigating biophysical and biochemical interactions between constituents of cell membranes and, for example, drugs and toxins altering membrane function. Designing and controlling the self-assembly of model membranes onto sensor substrates thus constitutes an important field of research, enabling applications in, e.g., drug screening, dynamic biointerfaces, artificial noses, and research on membrane-active antibiotics. The authors have developed and investigated the formation of strongly negatively charged supported lipid membranes which systematically mimic bacterial membrane composition on three important biosensor materials: SiO2, TiO2, and indium tin oxide. By tuning the electrostatic interaction through balancing the lipid vesicle charge with the ionic strength of Ca2+ as a fusion promoter, the authors have optimized the self-assembly and obtained new insights into the details of lipid vesicle-surface interaction. The results will be useful for future development and application of specialized lipid membrane surface coatings prepared from complex lipid compositions. The adsorption processes were characterized by a quartz crystal microbalance with dissipation monitoring, optical waveguide lightmode spectroscopy, and fluorescence recovery after photobleaching, which allowed the determination of formation also of nonplanar supported lipid membranes.

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“…A number of surface-sensitive techniques have been used to study the interaction of vesicle suspensions with solid surfaces, including electrochemical (10) and optical (5,(11)(12)(13) methods. Recently, acoustic wave devices have also been applied to these studies and have been shown to be able to monitor the mass and viscoelastic changes occurring during vesicle fusion on the device surface (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%