On the allergenicity of Hev b 1 among health care workers and patients with spina bifida allergic to natural rubber latex

Chen, Zhiping; Cremer, Reinhold; Posch, Anton; Raulf‐Heimsoth, Monika; Rihs, Hans‐Peter; Baur, Xaver

doi:10.1016/s0091-6749(97)70174-x

Cited by 88 publications

(68 citation statements)

References 21 publications

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“…On the other hand, 11 subjects with latex allergy had negative test results in immunoblotting; this could be due to the lack of relevant allergen in the antigen preparations used in our study, the lack of sensitivity of the immunoblot method or, finally, technical reasons could be an additional negative factor indicating the limitations of this assay. Recently some authors [15]have demonstrated by ELISA experiments that hevein, a domain in prohevein, is a major allergen in healthcare workers with latex allergy, and the 14-kD protein is the same for children with spina bifida [16]. …”

Section: Discussionmentioning

confidence: 99%

Contact Urticaria from Latex in Healthcare Workers

Valsecchi¹,

Leghissa

Cortinovis

et al. 2000

Dermatology

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Background: Latex allergy is an important medical problem for an increasing number of patients. It has been documented as causing immediate hypersensitivity reactions ranging from mild urticaria to life-threatening anaphylaxis after cutaneous, mucosal or visceral exposure. Recent studies in northern Europe and the USA suggest that between 2.8 and 16.9% of healthcare workers are affected by latex hypersensitivity type I reactions. Objectives: To test the prevalence of contact urticaria from latex gloves in a group of healthcare workers, to examine the factors associated with latex allergy and to evaluate some diagnostic methods used in latex allergy. Methods: A total of 929 employees of the surgical units who used latex gloves on a regular basis, at least once a day, were invited to participate in this study including administration of a questionnaire, a prick test with a commercial extract of latex, a prick test with latex glove eluate, a use test, RAST and an immunoblotting system; moreover, a prick test with a group of common inhalant allergens and a prick-by-prick test with fresh fruit (banana, kiwi, avocado, chestnut) were employed. Results: Of the 929 staff sent questionnaires, 313 (33.5%) replied; of those who responded, 118 gave a history of hand problems such as itch, erythema, wheals when wearing gloves, dryness and irritation most marked on the backs of the hands. Among these 118 workers, 16 refused skin testing and examination of blood, so 102 subjects were studied for latex allergy; 21/118 (17.8%) healthcare workers were found to be latex allergic. Eighty-one staff members gave a history of hand problems worsened by wearing gloves but were not latex allergic on testing. Those healthcare workers who completed the questionnaire and answered negatively (195/313) were not tested for latex allergy. Prick tests with the commercial solution were positive in 11 of the 21 subjects studied; prick tests with the eluate of glove, RAST and the use test were positive in all workers; 10 of the 21 sera showed positive immunoblot results. Atopy and a preexisting irritant contact eczema of the hands were present in a high percentage of the workers. Conclusion: In this study of healthcare personnel, we found that allergic contact urticaria from latex was present in 21 workers of the 313 (6.7%) who responded to the questionnaire and of the 102 (20.5%) who were tested for latex allergy. Atopy and irritant contact eczema of the hands were frequent in these subjects. Skin prick testing with latex glove eluate and the use test seem to be more sensitive than in vitro testing, particularly immunoblotting, and are biologically more relevant; skin testing with glove eluate must be preferred to testing with a commercial extract.

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Section: Discussionmentioning

confidence: 99%

Contact Urticaria from Latex in Healthcare Workers

Valsecchi¹,

Leghissa

Cortinovis

et al. 2000

Dermatology

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“…The WHO_International Union of Immunological Societies Allergen Nomenclature Committee (www.allergens.org) listed 14 NRL Hev b allergens (Hev b 1-14) characterized at the molecular level. It has included Hev b 1, rubber elongation factor (Chen et al, 1997); Hev b 2, b-1,3-glucanase (Yagami et al, 2002); Hev b 3, small rubber particle protein -a 24-kDa protein (Wagner et al, 1999); Hev b 4, a component of the microhelix protein complexlecithinase homologue; Hev b 6, prohevein/hevein precursor; Hev b 7; patatin-like protein; and Hev b 13, esterase (Beirnstein et al, 2003); Hev b 5, acidic protein -proline-rich protein (Slater et al, 1996); Hev b 8, profiling (Nieto et al, 2002); Hev b 9, enolase (Wagner et al, 2000); Hev b 10, manganese superoxide dismutase (Rihs et al, 2001); Hev b 12, non-specific lipid transfer protein 1 (Beezhold et al, 2003); and Hev b 11, class I chitinase, and Hev b 14, hevamine, that no allergenicity has been described yet. The epitopes identified as IgEbinding areas have been defined (Pedraza-Escalona et al, 2009;Rougé et al, 2010).…”

Section: Latex Allergensmentioning

confidence: 99%

“…Natural rubber latex (NRL) allergenic proteins are listed in Table 1. It has been suggested that Hev b 1 and Hev b 3 are major allergens in children with spina bifida and urological congenital anomalies (Baur et al, 1995;Yeang et al, 1996;Chen et al, 1997). Berstein et al (2003) identified Hev b 2, 5, 6.01 and Hev b 13 as the major in vivo allergens among HCWs with allergy to NRL, confirmed by percutaneous sensitivity to nonammoniated latex (NAL).…”

Section: Latex Allergensmentioning

confidence: 99%

“…Allergen name WM Allerginicity reference Rubber elongation factor Hev b 1 14 mainly associated with spina bifida patients Chen et al, 1997 b-1,3-glucanase Hev b 2 34 linked more to adult latex-allergy patients Yagami et al, 2002 Small rubber particle protein Hev b 3 24 mainly associated with spina bifida patients Wagner et al, 1999 Lecithinase …”

Section: Biochemical Namementioning

confidence: 99%

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Natural Rubber Latex Allergy

Sell¹,

Visentainer²

2012

Allergic Diseases - Highlights in the Clinic, Mechanisms and Treatment

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“…There is evidence to suggest that different groups of patients may show different patterns of seropositivity and that this may reflect different patterns/routes of exposure of latex allergens, for example, Hev b 1 is a common allergen in children with spina bifida but not for healthcare workers. 32,16 Some of the antigens in NRL are nonspecific to NRL and may be present in other plants. These are frequently enzymes that are present in the H. brasiliensis plant and that are also present in other plants such as endochitinases.…”

Section: Epidemiologymentioning

confidence: 99%

Cataract surgery for natural rubber latex allergic patients

Cheung

Gillow²

2003

Eye

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The prevalence of reactions against natural rubber latex (NRL) is thought to be increasing in both the general public and healthcare workers. These can vary from mild benign skin reactions to bronchospasm, anaphylactic shock, and death. Difficulties exist for ophthalmic departments wishing to establish protocols in providing 'latex-free environments' for patients undergoing cataract surgery. Currently no legislation exists regarding the labelling of NRL-containing products in the United Kingdom with information on a product's NRL content provided by the manufacturer on a voluntary basis only. It is hoped this review article will act as a basic guide in the management of NRL-sensitive patients undergoing cataract surgery in the United Kingdom.

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On the allergenicity of Hev b 1 among health care workers and patients with spina bifida allergic to natural rubber latex

Cited by 88 publications

References 21 publications

Contact Urticaria from Latex in Healthcare Workers

Contact Urticaria from Latex in Healthcare Workers

Natural Rubber Latex Allergy

Cataract surgery for natural rubber latex allergic patients

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